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- PDB-4oi6: Crystal structure analysis of nickel-bound form SCO4226 from Stre... -
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Open data
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Basic information
Entry | Database: PDB / ID: 4oi6 | ||||||
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Title | Crystal structure analysis of nickel-bound form SCO4226 from Streptomyces coelicolor A3(2) | ||||||
![]() | Nickel responsive protein | ||||||
![]() | METAL BINDING PROTEIN / nickel responsive protein / Structural Genomics / ferredoxin-like fold / a nickel responsive protein / nickel binding | ||||||
Function / homology | ![]() ORF SCO4226, nickel-binding ferredoxin-like monomer / SCO4226-like / SCO4226, nickel-binding ferredoxin-like monomer / Nickel responsive protein SCO4226-like / Alpha-Beta Plaits / 2-Layer Sandwich / Alpha Beta Similarity search - Domain/homology | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Lu, M. / Jiang, Y.L. / Wang, S. / Cheng, W. / Zhang, R.G. / Virolle, M.J. / Chen, Y. / Zhou, C.Z. | ||||||
![]() | ![]() Title: Streptomyces coelicolor SCO4226 Is a Nickel Binding Protein. Authors: Lu, M. / Jiang, Y.L. / Wang, S. / Jin, H. / Zhang, R.G. / Virolle, M.J. / Chen, Y. / Zhou, C.Z. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 43.7 KB | Display | ![]() |
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PDB format | ![]() | 33.7 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 10211.600 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Details: pET22b / Source: (gene. exp.) ![]() ![]() ![]() #2: Chemical | ChemComp-NI / #3: Chemical | ChemComp-CIT / | #4: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 1.63 Å3/Da / Density % sol: 24.44 % |
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Crystal grow | Temperature: 289 K / Method: vapor diffusion, hanging drop / pH: 6.5 Details: 0.8 M sodium citrate dihydrate, pH 6.5 and 0.8 M NiSO4, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() |
Detector | Type: MAR scanner 345 mm plate / Detector: IMAGE PLATE / Date: Nov 13, 2008 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 2.04→17.536 Å / Num. all: 8509 / Num. obs: 8364 / % possible obs: 98.3 % / Observed criterion σ(F): 1.38 / Observed criterion σ(I): -3 |
Reflection shell | Resolution: 2.04→2.14 Å / % possible all: 88.6 |
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Processing
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Refinement | Method to determine structure: ![]()
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.04→17.536 Å
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Refine LS restraints |
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LS refinement shell |
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