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- PDB-4ohk: Human GKRP bound to AMG-2526 and S6P -

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Basic information

Entry
Database: PDB / ID: 4ohk
TitleHuman GKRP bound to AMG-2526 and S6P
ComponentsGlucokinase regulatory protein
KeywordsCARBOHYDRATE BINDING PROTEIN/INHIBITOR / Regulatory protein / Binds and inhibits GK (glucokinase) / glucokinase / nucleus / CARBOHYDRATE BINDING PROTEIN-INHIBITOR complex
Function / homology
Function and homology information


glucose sensor activity / fructose-6-phosphate binding / carbohydrate derivative metabolic process / negative regulation of glucokinase activity / kinase inhibitor activity / urate metabolic process / Regulation of Glucokinase by Glucokinase Regulatory Protein / Defective TPR may confer susceptibility towards thyroid papillary carcinoma (TPC) / response to fructose / regulation of glycolytic process ...glucose sensor activity / fructose-6-phosphate binding / carbohydrate derivative metabolic process / negative regulation of glucokinase activity / kinase inhibitor activity / urate metabolic process / Regulation of Glucokinase by Glucokinase Regulatory Protein / Defective TPR may confer susceptibility towards thyroid papillary carcinoma (TPC) / response to fructose / regulation of glycolytic process / triglyceride homeostasis / response to glucose / protein localization to nucleus / intracellular glucose homeostasis / protein import into nucleus / mitochondrion / nucleoplasm / cytosol / cytoplasm
Similarity search - Function
: / Glucokinase regulatory protein, second SIS domain / Glucokinase regulatory protein, conserved site / N-acetylmuramic acid 6-phosphate etherase/glucokinase regulatory protein / C-terminal lid domain of glucokinase regulatory protein / Glucokinase regulatory protein N-terminal SIS domain / Glucokinase regulatory protein family signature. / SIS domain / SIS domain profile. / SIS domain superfamily ...: / Glucokinase regulatory protein, second SIS domain / Glucokinase regulatory protein, conserved site / N-acetylmuramic acid 6-phosphate etherase/glucokinase regulatory protein / C-terminal lid domain of glucokinase regulatory protein / Glucokinase regulatory protein N-terminal SIS domain / Glucokinase regulatory protein family signature. / SIS domain / SIS domain profile. / SIS domain superfamily / Glucose-6-phosphate isomerase like protein; domain 1 / Rossmann fold / 3-Layer(aba) Sandwich / Alpha Beta
Similarity search - Domain/homology
Chem-2TE / IODIDE ION / D-SORBITOL-6-PHOSPHATE / Glucokinase regulatory protein
Similarity search - Component
Biological speciesHomo sapiens (human)
MethodX-RAY DIFFRACTION / MOLECULAR REPLACEMENT / Resolution: 2.8 Å
AuthorsJordan, S.R. / Chmait, S.
CitationJournal: J.Med.Chem. / Year: 2014
Title: Small molecule disruptors of the glucokinase-glucokinase regulatory protein interaction: 3. Structure-activity relationships within the aryl carbinol region of the N-arylsulfonamido-N'-arylpiperazine series.
Authors: Nishimura, N. / Norman, M.H. / Liu, L. / Yang, K.C. / Ashton, K.S. / Bartberger, M.D. / Chmait, S. / Chen, J. / Cupples, R. / Fotsch, C. / Helmering, J. / Jordan, S.R. / Kunz, R.K. / ...Authors: Nishimura, N. / Norman, M.H. / Liu, L. / Yang, K.C. / Ashton, K.S. / Bartberger, M.D. / Chmait, S. / Chen, J. / Cupples, R. / Fotsch, C. / Helmering, J. / Jordan, S.R. / Kunz, R.K. / Pennington, L.D. / Poon, S.F. / Siegmund, A. / Sivits, G. / Lloyd, D.J. / Hale, C. / St Jean, D.J.
History
DepositionJan 17, 2014Deposition site: RCSB / Processing site: RCSB
Revision 1.0Oct 8, 2014Provider: repository / Type: Initial release
Revision 1.1Sep 20, 2023Group: Data collection / Database references ...Data collection / Database references / Derived calculations / Refinement description / Structure summary
Category: chem_comp / chem_comp_atom ...chem_comp / chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model / struct_ref_seq_dif / struct_site
Item: _chem_comp.pdbx_synonyms / _database_2.pdbx_DOI ..._chem_comp.pdbx_synonyms / _database_2.pdbx_DOI / _database_2.pdbx_database_accession / _struct_ref_seq_dif.details / _struct_site.pdbx_auth_asym_id / _struct_site.pdbx_auth_comp_id / _struct_site.pdbx_auth_seq_id

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Glucokinase regulatory protein
B: Glucokinase regulatory protein
hetero molecules


Theoretical massNumber of molelcules
Total (without water)145,70435
Polymers140,6132
Non-polymers5,09033
Water1,78399
1
A: Glucokinase regulatory protein
hetero molecules


Theoretical massNumber of molelcules
Total (without water)72,59815
Polymers70,3071
Non-polymers2,29214
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
MethodPISA
2
B: Glucokinase regulatory protein
hetero molecules


Theoretical massNumber of molelcules
Total (without water)73,10520
Polymers70,3071
Non-polymers2,79919
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
MethodPISA
Unit cell
Length a, b, c (Å)149.590, 149.590, 132.651
Angle α, β, γ (deg.)90.00, 90.00, 120.00
Int Tables number170
Space group name H-MP65

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Components

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Protein / Sugars , 2 types, 4 molecules AB

#1: Protein Glucokinase regulatory protein / Glucokinase regulator


Mass: 70306.617 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: GCKR / Production host: Spodoptera frugiperda (fall armyworm) / References: UniProt: Q14397
#3: Sugar ChemComp-S6P / D-SORBITOL-6-PHOSPHATE / 1-O-PHOSPHONO-D-GLUCITOL / D-GLUCITOL-6-PHOSPHATE


Type: D-saccharide / Mass: 262.152 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Formula: C6H15O9P

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Non-polymers , 5 types, 130 molecules

#2: Chemical ChemComp-2TE / (2R)-2-{4-[(2S)-4-[(6-aminopyridin-3-yl)sulfonyl]-2-(prop-1-yn-1-yl)piperazin-1-yl]phenyl}-1,1,1-trifluorohex-4-yn-2-ol


Mass: 506.541 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: C24H25F3N4O3S
#4: Chemical...
ChemComp-IOD / IODIDE ION


Mass: 126.904 Da / Num. of mol.: 25 / Source method: obtained synthetically / Formula: I
#5: Chemical ChemComp-GOL / GLYCEROL / GLYCERIN / PROPANE-1,2,3-TRIOL


Mass: 92.094 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C3H8O3
#6: Chemical ChemComp-SO4 / SULFATE ION


Mass: 96.063 Da / Num. of mol.: 3 / Source method: obtained synthetically / Formula: SO4
#7: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 99 / Source method: isolated from a natural source / Formula: H2O

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 3.05 Å3/Da / Density % sol: 59.63 %
Crystal growTemperature: 298 K / Method: vapor diffusion, hanging drop / pH: 7.5
Details: .1M Bis-tris, .2M NaI, 16%PEG8000, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K

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Data collection

DiffractionMean temperature: 100 K
Diffraction sourceSource: ROTATING ANODE / Type: RIGAKU FR-E SUPERBRIGHT / Wavelength: 1.5418 Å
DetectorType: RIGAKU SATURN 92 / Detector: CCD / Date: Apr 21, 2012 / Details: Osmic mirrors
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 1.5418 Å / Relative weight: 1
ReflectionResolution: 2.8→50 Å / Num. all: 41546 / Num. obs: 38423 / % possible obs: 92 % / Observed criterion σ(F): 1

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Processing

Software
NameVersionClassification
HKL-2000data collection
AMoREphasing
REFMAC5.8.0049refinement
HKL-2000data reduction
HKL-2000data scaling
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: PDB ENTRY 4LY9

4ly9


Resolution: 2.8→29.72 Å / Cor.coef. Fo:Fc: 0.936 / Cor.coef. Fo:Fc free: 0.887 / SU B: 15.473 / SU ML: 0.299 / Cross valid method: THROUGHOUT / ESU R: 1.764 / ESU R Free: 0.4 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
RfactorNum. reflection% reflectionSelection details
Rfree0.28289 2039 5 %RANDOM
Rwork0.21423 ---
obs0.21771 38423 97.31 %-
Solvent computationIon probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK
Displacement parametersBiso mean: 59.349 Å2
Baniso -1Baniso -2Baniso -3
1-0.01 Å20 Å20 Å2
2--0.01 Å2-0 Å2
3----0.03 Å2
Refinement stepCycle: LAST / Resolution: 2.8→29.72 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms9075 0 148 99 9322
Refine LS restraints
Refine-IDTypeDev idealDev ideal targetNumber
X-RAY DIFFRACTIONr_bond_refined_d0.0120.0199361
X-RAY DIFFRACTIONr_bond_other_d0.0010.029169
X-RAY DIFFRACTIONr_angle_refined_deg1.6491.9812677
X-RAY DIFFRACTIONr_angle_other_deg0.8523.00121026
X-RAY DIFFRACTIONr_dihedral_angle_1_deg7.02451169
X-RAY DIFFRACTIONr_dihedral_angle_2_deg35.57324.415376
X-RAY DIFFRACTIONr_dihedral_angle_3_deg18.6151658
X-RAY DIFFRACTIONr_dihedral_angle_4_deg20.81550
X-RAY DIFFRACTIONr_chiral_restr0.0810.21487
X-RAY DIFFRACTIONr_gen_planes_refined0.0070.02110380
X-RAY DIFFRACTIONr_gen_planes_other0.0010.022038
X-RAY DIFFRACTIONr_nbd_refined
X-RAY DIFFRACTIONr_nbd_other
X-RAY DIFFRACTIONr_nbtor_refined
X-RAY DIFFRACTIONr_nbtor_other
X-RAY DIFFRACTIONr_xyhbond_nbd_refined
X-RAY DIFFRACTIONr_xyhbond_nbd_other
X-RAY DIFFRACTIONr_metal_ion_refined
X-RAY DIFFRACTIONr_metal_ion_other
X-RAY DIFFRACTIONr_symmetry_vdw_refined
X-RAY DIFFRACTIONr_symmetry_vdw_other
X-RAY DIFFRACTIONr_symmetry_hbond_refined
X-RAY DIFFRACTIONr_symmetry_hbond_other
X-RAY DIFFRACTIONr_symmetry_metal_ion_refined
X-RAY DIFFRACTIONr_symmetry_metal_ion_other
X-RAY DIFFRACTIONr_mcbond_it4.1265.8154694
X-RAY DIFFRACTIONr_mcbond_other4.1255.8154693
X-RAY DIFFRACTIONr_mcangle_it6.4058.7085857
X-RAY DIFFRACTIONr_mcangle_other6.4048.7095858
X-RAY DIFFRACTIONr_scbond_it3.9566.1164667
X-RAY DIFFRACTIONr_scbond_other3.9046.1114655
X-RAY DIFFRACTIONr_scangle_it
X-RAY DIFFRACTIONr_scangle_other6.1259.0456803
X-RAY DIFFRACTIONr_long_range_B_refined8.85545.91910796
X-RAY DIFFRACTIONr_long_range_B_other8.85645.92110797
X-RAY DIFFRACTIONr_rigid_bond_restr
X-RAY DIFFRACTIONr_sphericity_free
X-RAY DIFFRACTIONr_sphericity_bonded
LS refinement shellResolution: 2.8→2.871 Å / Total num. of bins used: 20
RfactorNum. reflection% reflection
Rfree0.433 159 -
Rwork0.329 2876 -
obs--99.22 %

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