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- PDB-4o65: Crystal structure of the cupredoxin domain of amoB from Nitrosoca... -
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Open data
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Basic information
Entry | Database: PDB / ID: 4o65 | ||||||
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Title | Crystal structure of the cupredoxin domain of amoB from Nitrosocaldus yellowstonii | ||||||
![]() | Putative archaeal ammonia monooxygenase subunit B | ||||||
![]() | OXIDOREDUCTASE / Cupredoxin / ammonia monooxygenase / AMO / Ammonia oxidation / Ammonia / Membrane | ||||||
Function / homology | ![]() | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Lawton, T.J. / Ham, J. / Sun, T. / Rosenzweig, A.C. | ||||||
![]() | ![]() Title: Structural conservation of the B subunit in the ammonia monooxygenase/particulate methane monooxygenase superfamily. Authors: Lawton, T.J. / Ham, J. / Sun, T. / Rosenzweig, A.C. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 76.8 KB | Display | ![]() |
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PDB format | ![]() | 61.5 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 439.1 KB | Display | ![]() |
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Full document | ![]() | 439.1 KB | Display | |
Data in XML | ![]() | 10.1 KB | Display | |
Data in CIF | ![]() | 14.1 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Components on special symmetry positions |
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Components
#1: Protein | Mass: 18156.414 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Gene: amoB / Production host: ![]() ![]() | ||
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#2: Chemical | ChemComp-CU / | ||
#3: Chemical | #4: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.04 Å3/Da / Density % sol: 59.55 % |
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Crystal grow | Temperature: 277.15 K / Method: vapor diffusion / pH: 4 Details: 1 M NH4SO4, 100 mM sodium formate pH 4.0, and 2% polyethylene glycol 8000, VAPOR DIFFUSION, temperature 277.15K |
-Data collection
Diffraction | Mean temperature: 77 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARMOSAIC 300 mm CCD / Detector: CCD / Date: Jun 13, 2010 |
Radiation | Monochromator: Si(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97856 Å / Relative weight: 1 |
Reflection | Resolution: 1.796→22.43 Å / Num. all: 20725 / Num. obs: 20852 / % possible obs: 99.3 % / Observed criterion σ(F): 2 / Observed criterion σ(I): 2 |
Reflection shell | Resolution: 1.796→7.842 Å / % possible all: 98.37 |
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Processing
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Refinement | Method to determine structure: ![]()
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 28.035 Å2
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Refinement step | Cycle: LAST / Resolution: 1.796→22.43 Å
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Refine LS restraints |
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