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Open data
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Basic information
Entry | Database: PDB / ID: 4o60 | ||||||
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Title | Structure of ankyrin repeat protein | ||||||
![]() | ANK-N5C-317 | ||||||
![]() | DE NOVO PROTEIN / ANKYRIN / DESIGNED ANKYRIN REPEATS | ||||||
Function / homology | Ankyrin repeat-containing domain / Serine Threonine Protein Phosphatase 5, Tetratricopeptide repeat / Alpha Horseshoe / Mainly Alpha![]() | ||||||
Biological species | synthetic construct (others) | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Ethayathulla, A.S. / Guan, L. | ||||||
![]() | ![]() Title: A transcription blocker isolated from a designed repeat protein combinatorial library by in vivo functional screen. Authors: Tikhonova, E.B. / Ethayathulla, A.S. / Su, Y. / Hariharan, P. / Xie, S. / Guan, L. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 93.6 KB | Display | ![]() |
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PDB format | ![]() | 74 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 419.9 KB | Display | ![]() |
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Full document | ![]() | 424 KB | Display | |
Data in XML | ![]() | 17.3 KB | Display | |
Data in CIF | ![]() | 23.7 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 4qfvC ![]() 2xeeS C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 25223.826 Da / Num. of mol.: 2 / Source method: obtained synthetically / Details: SYNTHETIC CONSTRUCT / Source: (synth.) synthetic construct (others) #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.04 Å3/Da / Density % sol: 39.64 % |
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Crystal grow | Temperature: 298 K / pH: 4.2 Details: 100 mM sodium acetate trihydrate (pH 4.5), 200 mM (NH3)2SO4, 16-18% PEG 4000, and 10% glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
-Data collection
Diffraction | Mean temperature: 300 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 315r / Detector: CCD / Date: Oct 15, 2013 / Details: MIRROR |
Radiation | Monochromator: GRAPHITE / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97 Å / Relative weight: 1 |
Reflection | Resolution: 2.5→40 Å / Num. obs: 27744 / % possible obs: 99.9 % / Observed criterion σ(I): 2 / Redundancy: 6.4 % / Rsym value: 0.09 / Net I/σ(I): 30 |
Reflection shell | Resolution: 2.5→2.59 Å / Redundancy: 7 % / Rsym value: 0.7 / % possible all: 100 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 2XEE Resolution: 2.52→38.31 Å / SU ML: 0.33 / σ(F): 1.39 / Phase error: 25.72 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.52→38.31 Å
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Refine LS restraints |
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LS refinement shell |
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