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- PDB-4mis: The structure of Brucella abortus PliC in the orthorombic crystal form -
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Open data
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Basic information
Entry | Database: PDB / ID: 4mis | ||||||
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Title | The structure of Brucella abortus PliC in the orthorombic crystal form | ||||||
![]() | Putative uncharacterized protein | ||||||
![]() | HYDROLASE INHIBITOR / ![]() | ||||||
Function / homology | C-type lysozyme inhibitor / Membrane-bound lysozyme-inhibitor of c-type lysozyme / C-type lysozyme inhibitor / C-type lysozyme inhibitor superfamily / ![]() ![]() ![]() ![]() | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Ha, N.C. / Um, S.H. / Kim, J.S. | ||||||
![]() | ![]() Title: Structural basis for the inhibition of human lysozyme by PliC from Brucella abortus Authors: Um, S.H. / Kim, J.S. / Kim, K. / Kim, N. / Cho, H.S. / Ha, N.C. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 46.9 KB | Display | ![]() |
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PDB format | ![]() | 36.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 10646.972 Da / Num. of mol.: 2 / Fragment: UNP residues 27-121 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() #2: Water | ChemComp-HOH / | ![]() |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.29 Å3/Da / Density % sol: 62.56 % |
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Crystal grow![]() | Temperature: 287.15 K / Method: vapor diffusion, hanging drop / pH: 8.5 Details: 1.9M ammonium sulfate, 0.1M Tris-HCl, 10% glycerol, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 287.15K |
-Data collection
Diffraction | Mean temperature: 100 K | |||||||||||||||
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Diffraction source | Source: ![]() ![]() | |||||||||||||||
Detector | Type: ADSC QUANTUM 315 / Detector: CCD / Date: Dec 16, 2012 | |||||||||||||||
Radiation | Monochromator: double mirrors / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | |||||||||||||||
Radiation wavelength | Wavelength![]() | |||||||||||||||
Reflection | Resolution: 2→50 Å / Num. all: 19081 / Num. obs: 18850 / % possible obs: 96.1 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 | |||||||||||||||
Reflection shell |
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Processing
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Refinement | Method to determine structure![]() ![]()
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2→19.601 Å
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Refine LS restraints |
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LS refinement shell | Refine-ID: X-RAY DIFFRACTION / Total num. of bins used: 7
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