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Open data
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Basic information
Entry | Database: PDB / ID: 4m9v | |||||||||
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Title | Zfp57 mutant (E182Q) in complex with 5-carboxylcytosine DNA | |||||||||
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![]() | TRANSCRIPTION/DNA / epigenetics / transcription factor / 5-carboxylcytosine / C2H2 Zinc finger / DNA binding / TRANSCRIPTION-DNA complex | |||||||||
Function / homology | ![]() epigenetic programing of female pronucleus / epigenetic programming of gene expression / double-stranded methylated DNA binding / genomic imprinting / autosome genomic imprinting / epigenetic programming in the zygotic pronuclei / negative regulation of gene expression via chromosomal CpG island methylation / heterochromatin / chromatin binding / negative regulation of transcription by RNA polymerase II ...epigenetic programing of female pronucleus / epigenetic programming of gene expression / double-stranded methylated DNA binding / genomic imprinting / autosome genomic imprinting / epigenetic programming in the zygotic pronuclei / negative regulation of gene expression via chromosomal CpG island methylation / heterochromatin / chromatin binding / negative regulation of transcription by RNA polymerase II / DNA binding / nucleus / metal ion binding Similarity search - Function | |||||||||
Biological species | ![]() ![]() | |||||||||
Method | ![]() ![]() ![]() | |||||||||
![]() | Liu, Y. / Olanrewaju, Y.O. / Zhang, X. / Cheng, X. | |||||||||
![]() | ![]() Title: DNA recognition of 5-carboxylcytosine by a zfp57 mutant at an atomic resolution of 0.97 angstrom. Authors: Liu, Y. / Olanrewaju, Y.O. / Zhang, X. / Cheng, X. | |||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 178.1 KB | Display | ![]() |
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PDB format | ![]() | 139.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 487.5 KB | Display | ![]() |
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Full document | ![]() | 492.9 KB | Display | |
Data in XML | ![]() | 16.8 KB | Display | |
Data in CIF | ![]() | 25 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 4gznS S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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2 | ![]()
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Unit cell |
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Components
-DNA chain , 2 types, 4 molecules ADBE
#1: DNA chain | Mass: 3363.224 Da / Num. of mol.: 2 / Source method: obtained synthetically / Details: DNA synthesis #2: DNA chain | Mass: 3402.221 Da / Num. of mol.: 2 / Source method: obtained synthetically / Details: DNA synthesis |
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-Protein , 1 types, 2 molecules CF
#3: Protein | Mass: 7376.424 Da / Num. of mol.: 2 / Mutation: E182Q Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
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-Non-polymers , 5 types, 486 molecules ![](data/chem/img/MPD.gif)
![](data/chem/img/ACT.gif)
![](data/chem/img/CA.gif)
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![](data/chem/img/HOH.gif)
![](data/chem/img/ACT.gif)
![](data/chem/img/CA.gif)
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![](data/chem/img/HOH.gif)
#4: Chemical | ChemComp-MPD / ( #5: Chemical | ChemComp-ACT / #6: Chemical | #7: Chemical | ChemComp-ZN / #8: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.07 Å3/Da / Density % sol: 40.56 % |
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Crystal grow | Temperature: 289 K / Method: vapor diffusion, sitting drop / pH: 4.2 Details: 35% 2-methyl-2,4-pentanediol, 15% polyethylene glycol 8000, 100 mM CaCl2, and 100 mM acetate, pH 4.2, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARMOSAIC 300 mm CCD / Detector: CCD / Date: Dec 31, 2012 Details: Si 111. Rosenbaum-Rock double-crystal monochromator: liquid nitrogen cooled; sagitally focusing 2nd crystal, Rosenbaum-Rock vertical focusing mirror |
Radiation | Monochromator: double crystal - liquid nitrogen cooled / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.8 Å / Relative weight: 1 |
Reflection | Resolution: 0.969→33.5 Å / Num. obs: 129957 / % possible obs: 95.7 % / Observed criterion σ(I): -3 / Redundancy: 2.6 % / Biso Wilson estimate: 8.63 Å2 / Rmerge(I) obs: 0.07 / Net I/σ(I): 11.7 |
Reflection shell | Resolution: 0.969→1 Å / Redundancy: 2.2 % / Rmerge(I) obs: 0.648 / Mean I/σ(I) obs: 1.4 / Num. unique all: 12599 / % possible all: 92.7 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 4GZN Resolution: 0.969→33.459 Å / SU ML: 0.08 / Cross valid method: THROUGHOUT / σ(F): 1.34 / Phase error: 14.11 / Stereochemistry target values: ML / Details: FLAT BULK SOLVENT MODEL
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 13.7 Å2 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 0.969→33.459 Å
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Refine LS restraints |
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LS refinement shell | Refine-ID: X-RAY DIFFRACTION
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