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- PDB-4m0v: Crystal structure of E.coli SbcD with Mn2+ -

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Basic information

Entry
Database: PDB / ID: 4m0v
TitleCrystal structure of E.coli SbcD with Mn2+
ComponentsExonuclease subunit SbcD
KeywordsHYDROLASE / meiotic recombination 11 homolog / double-strand break repair protein / nuclease / endonuclease / exonuclease
Function / homologyDouble Stranded RNA Binding Domain - #720 / Metallo-dependent phosphatases / Purple Acid Phosphatase; chain A, domain 2 / Double Stranded RNA Binding Domain / 4-Layer Sandwich / 2-Layer Sandwich / Alpha Beta / : / :
Function and homology information
Biological speciesEscherichia coli (E. coli)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.83 Å
AuthorsLiu, S. / Tian, L.F. / Yan, X.X. / Liang, D.C.
CitationJournal: Acta Crystallogr.,Sect.D / Year: 2014
Title: Structural basis for DNA recognition and nuclease processing by the Mre11 homologue SbcD in double-strand breaks repair.
Authors: Liu, S. / Tian, L.F. / Liu, Y.P. / An, X.M. / Tang, Q. / Yan, X.X. / Liang, D.C.
History
DepositionAug 2, 2013Deposition site: RCSB / Processing site: PDBJ
Revision 1.0Feb 26, 2014Provider: repository / Type: Initial release
Revision 1.1Nov 8, 2023Group: Data collection / Database references ...Data collection / Database references / Derived calculations / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model / pdbx_struct_conn_angle / struct_conn / struct_ref_seq_dif / struct_site
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession ..._database_2.pdbx_DOI / _database_2.pdbx_database_accession / _pdbx_struct_conn_angle.ptnr1_auth_asym_id / _pdbx_struct_conn_angle.ptnr1_auth_comp_id / _pdbx_struct_conn_angle.ptnr1_auth_seq_id / _pdbx_struct_conn_angle.ptnr1_label_asym_id / _pdbx_struct_conn_angle.ptnr1_label_atom_id / _pdbx_struct_conn_angle.ptnr1_label_comp_id / _pdbx_struct_conn_angle.ptnr1_label_seq_id / _pdbx_struct_conn_angle.ptnr2_auth_asym_id / _pdbx_struct_conn_angle.ptnr2_auth_seq_id / _pdbx_struct_conn_angle.ptnr2_label_asym_id / _pdbx_struct_conn_angle.ptnr3_auth_asym_id / _pdbx_struct_conn_angle.ptnr3_auth_comp_id / _pdbx_struct_conn_angle.ptnr3_auth_seq_id / _pdbx_struct_conn_angle.ptnr3_label_asym_id / _pdbx_struct_conn_angle.ptnr3_label_atom_id / _pdbx_struct_conn_angle.ptnr3_label_comp_id / _pdbx_struct_conn_angle.ptnr3_label_seq_id / _pdbx_struct_conn_angle.value / _struct_conn.pdbx_dist_value / _struct_conn.ptnr1_auth_asym_id / _struct_conn.ptnr1_auth_comp_id / _struct_conn.ptnr1_auth_seq_id / _struct_conn.ptnr1_label_asym_id / _struct_conn.ptnr1_label_atom_id / _struct_conn.ptnr1_label_comp_id / _struct_conn.ptnr1_label_seq_id / _struct_conn.ptnr2_auth_asym_id / _struct_conn.ptnr2_auth_comp_id / _struct_conn.ptnr2_auth_seq_id / _struct_conn.ptnr2_label_asym_id / _struct_conn.ptnr2_label_atom_id / _struct_conn.ptnr2_label_comp_id / _struct_ref_seq_dif.details / _struct_site.pdbx_auth_asym_id / _struct_site.pdbx_auth_comp_id / _struct_site.pdbx_auth_seq_id

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Exonuclease subunit SbcD
B: Exonuclease subunit SbcD
C: Exonuclease subunit SbcD
D: Exonuclease subunit SbcD
hetero molecules


Theoretical massNumber of molelcules
Total (without water)158,67816
Polymers157,8704
Non-polymers80812
Water15,061836
1
A: Exonuclease subunit SbcD
hetero molecules


Theoretical massNumber of molelcules
Total (without water)39,6704
Polymers39,4681
Non-polymers2023
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
2
B: Exonuclease subunit SbcD
hetero molecules


Theoretical massNumber of molelcules
Total (without water)39,6704
Polymers39,4681
Non-polymers2023
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
3
C: Exonuclease subunit SbcD
hetero molecules


Theoretical massNumber of molelcules
Total (without water)39,6704
Polymers39,4681
Non-polymers2023
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
4
D: Exonuclease subunit SbcD
hetero molecules


Theoretical massNumber of molelcules
Total (without water)39,6704
Polymers39,4681
Non-polymers2023
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Unit cell
Length a, b, c (Å)62.162, 69.229, 95.187
Angle α, β, γ (deg.)72.24, 84.00, 83.48
Int Tables number1
Space group name H-MP1

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Components

#1: Protein
Exonuclease subunit SbcD / Nuclease SbcCD / D subunit


Mass: 39467.609 Da / Num. of mol.: 4 / Fragment: UNP RESIDUES 1-340
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Strain: ATCC 55124 / KO11 / Gene: sbcD, EKO11_3451, KO11_21615 / Production host: Escherichia coli (E. coli) / References: UniProt: E8Y9D8
#2: Chemical
ChemComp-GOL / GLYCEROL / GLYCERIN / PROPANE-1,2,3-TRIOL


Mass: 92.094 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: C3H8O3
#3: Chemical
ChemComp-MN / MANGANESE (II) ION


Mass: 54.938 Da / Num. of mol.: 8 / Source method: obtained synthetically / Formula: Mn
#4: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 836 / Source method: isolated from a natural source / Formula: H2O

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.45 Å3/Da / Density % sol: 49.76 %
Crystal growTemperature: 293 K / Method: vapor diffusion, hanging drop / pH: 7.5
Details: pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K

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Data collection

DiffractionMean temperature: 100 K
Diffraction sourceSource: SYNCHROTRON / Site: Photon Factory / Beamline: BL-17A / Wavelength: 0.9788 Å
DetectorDate: Jun 20, 2013
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.9788 Å / Relative weight: 1
ReflectionResolution: 1.83→50 Å / Num. obs: 128912
Reflection shellResolution: 1.83→1.86 Å

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Processing

Software
NameVersionClassification
MAR345dtbdata collection
PHASESphasing
PHENIX(phenix.refine: 1.7.3_928)refinement
HKL-2000data reduction
HKL-2000data scaling
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 4LTY

4lty


Resolution: 1.83→19.967 Å / SU ML: 0.23 / σ(F): 1.96 / Phase error: 20.21 / Stereochemistry target values: ML
RfactorNum. reflection% reflection
Rfree0.1986 6327 4.91 %
Rwork0.1618 --
obs0.1636 128912 97.7 %
Solvent computationShrinkage radii: 0.86 Å / VDW probe radii: 1.1 Å / Solvent model: FLAT BULK SOLVENT MODEL / Bsol: 48.245 Å2 / ksol: 0.348 e/Å3
Displacement parameters
Baniso -1Baniso -2Baniso -3
1-4.9813 Å2-0.5368 Å24.5863 Å2
2---4.4572 Å20.8469 Å2
3----0.6242 Å2
Refinement stepCycle: LAST / Resolution: 1.83→19.967 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms10234 0 32 836 11102
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.00710737
X-RAY DIFFRACTIONf_angle_d1.13214727
X-RAY DIFFRACTIONf_dihedral_angle_d15.0083770
X-RAY DIFFRACTIONf_chiral_restr0.0791713
X-RAY DIFFRACTIONf_plane_restr0.0051936
LS refinement shell

Refine-ID: X-RAY DIFFRACTION / Total num. of bins used: 30

Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection Rwork% reflection obs (%)
1.83-1.85080.26982290.2371392496
1.8508-1.87260.2572100.2177407896
1.8726-1.89540.26852240.2127399796
1.8954-1.91930.2541830.1938408197
1.9193-1.94460.22682320.1869405197
1.9446-1.97120.23612140.1762400297
1.9712-1.99930.22671940.1691408997
1.9993-2.02910.22992180.1683403697
2.0291-2.06080.21362100.1618405597
2.0608-2.09460.21642180.16406797
2.0946-2.13060.2282220.1549406797
2.1306-2.16930.1942210.1504404397
2.1693-2.2110.19492330.1474405497
2.211-2.25610.20442110.1494409698
2.2561-2.30510.20762220.1496409898
2.3051-2.35860.22731820.1597409398
2.3586-2.41750.21792130.1644406898
2.4175-2.48280.19471910.1578413298
2.4828-2.55570.20681860.1479410898
2.5557-2.6380.19432180.15412798
2.638-2.7320.18851980.1535411498
2.732-2.84110.21272240.1651411098
2.8411-2.970.19512110.1671413999
2.97-3.12610.21451980.1646413599
3.1261-3.32110.20231930.1636413599
3.3211-3.57620.20492340.1591411799
3.5762-3.93360.16322230.1412416399
3.9336-4.49710.15982280.1353412999
4.4971-5.64460.14881970.135417799
5.6446-19.96780.21181900.2316410098
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
13.42151.1875-1.13364.9955-2.06524.93650.1591-0.33970.14580.076-0.1012-0.2433-0.50050.73410.00370.203-0.07250.02210.2427-0.08930.124716.070581.962990.0472
21.9140.1237-0.77142.921-0.54822.22290.0458-0.05370.0828-0.11710.0463-0.2057-0.1610.299-0.0760.1316-0.02490.01660.1622-0.03750.10713.908178.82183.9482
38.5263-4.95152.6482.0008-1.48952.0023-0.0934-0.1836-0.2480.1409-0.2592-0.83680.53651.23140.37630.25530.10050.0170.44150.02080.193421.610872.613784.659
43.5489-2.3388-0.24127.7576-0.35775.1578-0.02960.0104-0.1788-0.20040.0167-0.00270.35490.08470.02630.1199-0.01420.01760.153-0.00720.09319.350571.737380.6805
54.93450.53323.2951.90011.68723.1762-0.01790.4341-0.43480.11550.3061-0.15410.34010.407-0.27320.27560.02040.05250.2738-0.04250.23727.603563.008991.3405
64.3478-0.9461-0.00254.1483-0.22793.56070.08280.29230.101-0.17330.05680.3535-0.1454-0.5123-0.14140.1640.01310.03680.25760.0060.1246-5.136374.370390.1248
74.36130.359-1.83975.9192-1.7854.250.2316-0.20180.28580.40570.12520.4262-0.5628-0.2615-0.32390.19780.0290.06180.1916-0.00960.1439-1.766281.096497.498
81.78650.881-0.17828.9228-2.41041.55670.1807-0.44520.43680.73980.03680.125-0.99790.2202-0.21380.4997-0.06390.09350.3007-0.1130.213811.256286.932296.5926
96.90666.6425-3.57968.6658-5.20325.3660.3248-0.6993-0.01640.6372-0.2323-0.3589-0.7850.9147-0.03960.3742-0.12960.02020.4145-0.13220.171713.880881.4515100.9203
100.19530.6540.35823.37670.96420.6924-0.2208-0.23460.4180.30220.1889-0.2495-0.17530.1818-0.0031.3721-0.55610.1127-0.2714-0.62250.351218.0815108.651691.7949
110.3571-0.07460.72273.55591.14131.90930.2934-0.18170.16711.0238-0.080.4564-0.55-0.1167-0.1451.4228-0.1590.30.1892-0.16220.458111.4674116.4784.0941
129.00718.24964.25889.46312.96092.472-0.0148-0.02140.0647-0.05710.095-0.6831-0.34440.2723-0.13331.0487-0.32070.17860.0822-0.00240.310919.2853107.123284.907
131.6335-0.2736-0.12352.28821.04444.32520.02910.11920.0758-0.0818-0.02240.1819-0.0517-0.40060.01510.09370.00780.01540.18350.04430.125831.004671.5683119.3018
142.01119.9536-6.60149.5251-3.3639.0446-0.07670.0008-0.1820.1446-0.03150.07460.0996-0.17790.0610.1530.0380.0030.14620.00460.151132.513459.6788125.4702
159.90655.09473.01915.86211.67722.27060.0922-0.2989-0.3320.181-0.1013-0.00430.4797-0.147-00.19780.02170.04290.16970.02660.1439.755157.8931116.0922
166.5366-6.334.23647.2916-2.92593.87960.0125-0.4165-0.1250.23630.1264-0.45690.26150.3037-0.14950.21410.1271-0.04930.40750.0520.33763.633460.4026126.0238
177.93356.96332.55026.12211.91862.0094-0.09780.1419-0.8392-0.27080.0418-0.76410.81820.63070.02940.24810.08460.04280.25020.03870.28254.291859.6281115.2638
183.4651.16050.36514.68750.84593.11380.02410.0809-0.0713-0.2247-0.0446-0.22420.14030.08120.020.14580.03820.03460.18480.03160.097546.880865.2132112.265
193.9883-1.4403-1.71712.6251.24233.40280.09450.3610.1563-0.2197-0.0459-0.1912-0.10630.1357-0.04730.1479-0.00430.04470.19650.04960.137946.69771.5087108.9047
201.03241.26472.28291.59422.63495.629-0.11250.2656-0.1581-0.8371-0.0495-0.1910.17210.36390.17070.63020.04620.21760.26370.12290.576238.140888.0503111.0482
211.3649-0.07790.13095.756-0.82180.44220.15730.36910.3191-0.6182-0.03110.0495-0.6959-0.1778-0.14380.47470.0450.06840.32020.14170.206433.624783.136106.7919
220.36691.531-0.60047.2502-1.43962.2342-0.13730.71860.865-0.53340.06390.2739-0.6918-0.21060.10690.75060.1490.01830.33120.16280.491127.518397.999115.0418
230.7493-0.05260.29440.33520.05840.5967-0.1222-0.15750.40760.304-0.0857-0.0956-0.27580.13060.14191.27250.09320.3220.08940.05440.754639.8487108.6743121.4815
240.45370.67531.70677.8584-0.70327.9694-0.32-0.09220.73050.0195-0.23040.1133-0.8102-0.37010.56890.7170.15510.12470.20110.03860.547330.90399.928119.7089
256.7661-5.0786-5.5832.00226.76075.3674-0.1003-0.2108-0.8768-0.04740.19560.64720.47060.0729-0.05310.2081-0.0024-0.04640.1427-0.00350.268232.353547.59759.3471
262.1199-1.4223-0.10712.62920.16150.7007-0.0284-0.0562-0.04520.06060.03540.14990.0574-0.0254-0.00940.1073-0.01390.00270.0928-0.00380.08314.190567.63558.4552
270.90661.9103-1.21275.3125-2.46052.04720.0455-0.09060.02960.2536-0.0558-0.0299-0.02460.06610.00130.11580.0135-0.00170.1413-0.01590.118719.80667.75565.7866
289.0176-9.3576-1.51412.01160.18552.0185-0.2916-0.42270.05021.0550.1020.1478-0.20440.13940.08710.2974-0.0329-0.02140.1815-0.00160.132822.512168.76973.7396
291.2622-0.85740.34071.4892-1.88663.07930.0066-0.11740.17750.2749-0.0583-0.3732-0.16720.15330.05840.1124-0.0134-0.00520.1485-0.02660.188932.261267.688664.1613
302.25212.9604-2.51923.8923-3.31242.82130.363-0.17160.78610.5115-0.344-0.5069-0.92150.5662-0.00040.3859-0.07290.03340.3674-0.00870.678140.834190.365154.4067
314.37851.3676-0.27792.0208-6.00088.12970.25380.08560.50170.271-0.2752-0.8883-0.44270.67410.06460.2091-0.04040.0610.22-0.02530.386541.490478.289754.0407
322.1013-1.0849-0.12833.4711-0.65860.89840.06030.16990.1092-0.2743-0.0711-0.3650.00080.12290.02320.1574-0.01280.03840.1242-0.01770.12428.918167.848549.8967
332.6043-0.23591.36561.2413-1.30582.00140.28180.7143-0.4876-0.7818-0.17220.59670.0893-0.1615-0.12710.43670.0483-0.15490.359-0.10050.20394.934867.892139.7246
347.6498-3.88414.51764.1952-1.02417.9904-0.11710.2782-1.078-0.4055-0.0799-0.05020.6011-0.85250.22390.254-0.0363-0.11240.2431-0.06790.306-1.017572.26342.5023
352.29881.6176-0.70068.7163-2.30981.84450.13230.15980.426-0.6375-0.0160.9659-0.1788-0.6594-0.08980.39520.0834-0.18320.50230.0820.6408-4.971383.442536.0371
369.13770.97538.57999.0457-2.77519.59090.02460.2861-0.4227-0.1410.017-0.0111-0.1568-0.21150.01520.23760.0334-0.03150.1891-0.02810.23130.739579.37242.7988
371.70841.03760.20572.67070.26990.8569-0.02260.11780.0029-0.07830.0060.05090.0065-0.01280.00980.09620.0180.01470.09290.00220.088822.471101.218152.741
381.6515-2.8849-0.26512.00125.32583.2321-0.01510.09680.32470.01650.1833-0.7847-0.0495-0.0113-0.10930.1407-0.0181-0.01260.14110.01530.22716.2074101.436348.3141
391.77392.08790.26896.97854.09873.88550.00520.02620.4026-0.3158-0.09660.5801-0.2763-0.12720.08990.09810.0344-0.0220.14990.04390.18698.7013108.986552.997
403.5737-4.82811.17499.4884-0.4640.8127-0.2399-0.29160.40490.3328-0.11190.5427-0.2004-0.33990.32120.4776-0.00960.13590.4335-0.10670.7394.8753130.342565.3745
417.55887.05944.42588.50727.71959.1178-0.0898-0.02810.5245-0.2899-0.27750.8961-0.3983-0.49960.36770.15630.068-0.01730.21310.0240.36030.0682111.451557.0396
423.83012.902-1.06735.7370.34610.7704-0.1106-0.070.24980.18440.04510.4941-0.08930.01350.06780.11820.03630.01990.08170.01720.140812.3317108.786163.7809
437.02234.2272-0.07028.16893.38172.24690.06-0.31980.37480.1882-0.15940.6086-0.1477-0.18560.10110.19570.02210.03540.11220.01760.10059.9041109.293966.3936
442.014-1.57220.60831.68760.98982.6679-0.1667-0.296-0.13190.28-0.01740.22160.1056-0.09760.21250.23840.00440.03660.1243-0.00870.144717.485102.939869.1213
453.4640.62731.59881.86650.54042.49650.1583-0.6892-0.22970.5349-0.1642-0.25680.08820.18140.00080.3036-0.0044-0.0360.25590.04770.155627.910297.254771.3376
465.48980.63550.12922.1014-3.11134.8161-0.0413-1.08270.01560.98340.0385-0.59020.20920.7686-0.04750.36150.0399-0.12740.4347-0.0340.248343.5178105.679775.4936
470.61890.09730.02510.0603-0.1420.4793-0.1059-0.0874-0.12370.2455-0.0343-0.737-0.23170.71460.12360.5502-0.0285-0.27760.8725-0.05311.109952.11108.625976.4427
488.8895.04975.23156.4115-0.37016.2241-0.2768-0.67790.54550.5823-0.16450.2022-0.38770.56110.42690.28810.0251-0.11020.3621-0.04030.323142.8926108.600572.8916
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection details
1X-RAY DIFFRACTION1(CHAIN A AND RESID -2:36)
2X-RAY DIFFRACTION2(CHAIN A AND RESID 37:64)
3X-RAY DIFFRACTION3(CHAIN A AND RESID 65:72)
4X-RAY DIFFRACTION4(CHAIN A AND RESID 73:107)
5X-RAY DIFFRACTION5(CHAIN A AND RESID 108:120)
6X-RAY DIFFRACTION6(CHAIN A AND RESID 121:202)
7X-RAY DIFFRACTION7(CHAIN A AND RESID 203:240)
8X-RAY DIFFRACTION8(CHAIN A AND RESID 241:261)
9X-RAY DIFFRACTION9(CHAIN A AND RESID 262:279)
10X-RAY DIFFRACTION10(CHAIN A AND RESID 280:309)
11X-RAY DIFFRACTION11(CHAIN A AND RESID 310:329)
12X-RAY DIFFRACTION12(CHAIN A AND RESID 330:338)
13X-RAY DIFFRACTION13(CHAIN B AND RESID -1:92)
14X-RAY DIFFRACTION14(CHAIN B AND RESID 93:107)
15X-RAY DIFFRACTION15(CHAIN B AND RESID 108:135)
16X-RAY DIFFRACTION16(CHAIN B AND RESID 136:153)
17X-RAY DIFFRACTION17(CHAIN B AND RESID 154:164)
18X-RAY DIFFRACTION18(CHAIN B AND RESID 165:202)
19X-RAY DIFFRACTION19(CHAIN B AND RESID 203:244)
20X-RAY DIFFRACTION20(CHAIN B AND RESID 245:252)
21X-RAY DIFFRACTION21(CHAIN B AND RESID 253:296)
22X-RAY DIFFRACTION22(CHAIN B AND RESID 297:310)
23X-RAY DIFFRACTION23(CHAIN B AND RESID 311:328)
24X-RAY DIFFRACTION24(CHAIN B AND RESID 330:338)
25X-RAY DIFFRACTION25(CHAIN C AND RESID -3:2)
26X-RAY DIFFRACTION26(CHAIN C AND RESID 3:63)
27X-RAY DIFFRACTION27(CHAIN C AND RESID 64:91)
28X-RAY DIFFRACTION28(CHAIN C AND RESID 92:102)
29X-RAY DIFFRACTION29(CHAIN C AND RESID 103:136)
30X-RAY DIFFRACTION30(CHAIN C AND RESID 137:147)
31X-RAY DIFFRACTION31(CHAIN C AND RESID 148:165)
32X-RAY DIFFRACTION32(CHAIN C AND RESID 166:265)
33X-RAY DIFFRACTION33(CHAIN C AND RESID 266:297)
34X-RAY DIFFRACTION34(CHAIN C AND RESID 299:310)
35X-RAY DIFFRACTION35(CHAIN C AND RESID 311:332)
36X-RAY DIFFRACTION36(CHAIN C AND RESID 333:339)
37X-RAY DIFFRACTION37(CHAIN D AND RESID -3:109)
38X-RAY DIFFRACTION38(CHAIN D AND RESID 110:117)
39X-RAY DIFFRACTION39(CHAIN D AND RESID 118:140)
40X-RAY DIFFRACTION40(CHAIN D AND RESID 141:151)
41X-RAY DIFFRACTION41(CHAIN D AND RESID 152:173)
42X-RAY DIFFRACTION42(CHAIN D AND RESID 174:201)
43X-RAY DIFFRACTION43(CHAIN D AND RESID 202:225)
44X-RAY DIFFRACTION44(CHAIN D AND RESID 226:247)
45X-RAY DIFFRACTION45(CHAIN D AND RESID 248:298)
46X-RAY DIFFRACTION46(CHAIN D AND RESID 299:317)
47X-RAY DIFFRACTION47(CHAIN D AND RESID 318:331)
48X-RAY DIFFRACTION48(CHAIN D AND RESID 332:340)

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