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- PDB-4jkr: Crystal Structure of E. coli RNA Polymerase in complex with ppGpp -

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Basic information

Entry
Database: PDB / ID: 4jkr
TitleCrystal Structure of E. coli RNA Polymerase in complex with ppGpp
Components
  • (DNA-directed RNA polymerase subunit ...) x 4
  • RNA polymerase sigma factor RpoD
KeywordsTRANSCRIPTION / transferase / RNA polymerase / transcription regulation
Function / homology
Function and homology information


: / : / : / : / : / : / sigma factor antagonist complex / regulation of DNA-templated transcription initiation / sigma factor activity / cytosolic DNA-directed RNA polymerase complex ...: / : / : / : / : / : / sigma factor antagonist complex / regulation of DNA-templated transcription initiation / sigma factor activity / cytosolic DNA-directed RNA polymerase complex / DNA-directed RNA polymerase complex / DNA-templated transcription initiation / ribonucleoside binding / DNA-directed RNA polymerase / response to heat / negative regulation of DNA-templated transcription / DNA binding / cytosol
Similarity search - Function
RNA polymerase sigma factor 70, non-essential domain / Sigma-70, non-essential region / RNA polymerase sigma factor 70, region 1.1 / Sigma-70 factor, region 1.1 superfamily / Sigma-70 factor, region 1.1 / Sigma-70 factors family signature 1. / RNA polymerase sigma factor RpoD, C-terminal / RNA polymerase sigma factor RpoD / : / RNA polymerase sigma-70 region 1.2 ...RNA polymerase sigma factor 70, non-essential domain / Sigma-70, non-essential region / RNA polymerase sigma factor 70, region 1.1 / Sigma-70 factor, region 1.1 superfamily / Sigma-70 factor, region 1.1 / Sigma-70 factors family signature 1. / RNA polymerase sigma factor RpoD, C-terminal / RNA polymerase sigma factor RpoD / : / RNA polymerase sigma-70 region 1.2 / Sigma-70 factor, region 1.2 / RNA polymerase sigma-70 region 3 / Sigma-70 region 3 / Sigma-70 factors family signature 2. / RNA polymerase sigma-70 / RNA polymerase sigma-70 region 4 / Sigma-70, region 4 / RNA polymerase sigma-70 region 2 / RNA polymerase sigma-70 like domain / Sigma-70 region 2 / RNA polymerase sigma factor, region 2 / RNA polymerase sigma factor, region 3/4-like / DNA-directed RNA polymerase, beta subunit, external 1 domain superfamily / DNA-directed RNA polymerase, beta subunit, external 1 domain / RNA polymerase beta subunit external 1 domain / DNA-directed RNA polymerase beta subunit, bacterial-type / RNA polymerase Rpb2, domain 2 superfamily / RNA polymerase, beta subunit, protrusion / RNA polymerase beta subunit / RNA polymerase Rpb2, domain 2 / RNA polymerase Rpb2, domain 2 / RNA polymerase, beta subunit, conserved site / RNA polymerase Rpb2, domain 7 / RNA polymerase Rpb2, domain 3 / RNA polymerase Rpb2, OB-fold / RNA polymerase Rpb2, domain 7 / RNA polymerase Rpb2, domain 3 / RNA polymerases beta chain signature. / DNA-directed RNA polymerase, subunit 2, hybrid-binding domain / DNA-directed RNA polymerase, subunit 2 / DNA-directed RNA polymerase, subunit 2, hybrid-binding domain superfamily / RNA polymerase Rpb2, domain 6 / Winged helix-like DNA-binding domain superfamily
Similarity search - Domain/homology
GUANOSINE-5',3'-TETRAPHOSPHATE / STRONTIUM ION / DNA-directed RNA polymerase subunit beta / : / : / : / : / RNA polymerase sigma factor RpoD
Similarity search - Component
Biological speciesEscherichia coli (E. coli)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 4.2 Å
AuthorsZuo, Y. / Wang, Y. / Steitz, T.A.
CitationJournal: Mol.Cell / Year: 2013
Title: The mechanism of E. coli RNA polymerase regulation by ppGpp is suggested by the structure of their complex.
Authors: Zuo, Y. / Wang, Y. / Steitz, T.A.
History
DepositionMar 11, 2013Deposition site: RCSB / Processing site: RCSB
Revision 1.0May 15, 2013Provider: repository / Type: Initial release
Revision 1.1Oct 23, 2013Group: Database references
Revision 1.2Feb 28, 2024Group: Data collection / Database references ...Data collection / Database references / Derived calculations / Structure summary
Category: chem_comp / chem_comp_atom ...chem_comp / chem_comp_atom / chem_comp_bond / database_2 / pdbx_struct_conn_angle / struct_conn / struct_ref_seq_dif / struct_site
Item: _chem_comp.pdbx_synonyms / _database_2.pdbx_DOI ..._chem_comp.pdbx_synonyms / _database_2.pdbx_DOI / _database_2.pdbx_database_accession / _pdbx_struct_conn_angle.ptnr1_auth_asym_id / _pdbx_struct_conn_angle.ptnr1_auth_seq_id / _pdbx_struct_conn_angle.ptnr1_label_asym_id / _pdbx_struct_conn_angle.ptnr1_label_seq_id / _pdbx_struct_conn_angle.ptnr2_auth_asym_id / _pdbx_struct_conn_angle.ptnr2_auth_seq_id / _pdbx_struct_conn_angle.ptnr2_label_asym_id / _pdbx_struct_conn_angle.ptnr3_auth_asym_id / _pdbx_struct_conn_angle.ptnr3_auth_seq_id / _pdbx_struct_conn_angle.ptnr3_label_asym_id / _pdbx_struct_conn_angle.ptnr3_label_seq_id / _pdbx_struct_conn_angle.value / _struct_conn.pdbx_dist_value / _struct_conn.ptnr1_auth_asym_id / _struct_conn.ptnr1_auth_comp_id / _struct_conn.ptnr1_auth_seq_id / _struct_conn.ptnr1_label_asym_id / _struct_conn.ptnr1_label_atom_id / _struct_conn.ptnr1_label_comp_id / _struct_conn.ptnr1_label_seq_id / _struct_conn.ptnr2_auth_asym_id / _struct_conn.ptnr2_auth_comp_id / _struct_conn.ptnr2_auth_seq_id / _struct_conn.ptnr2_label_asym_id / _struct_conn.ptnr2_label_atom_id / _struct_conn.ptnr2_label_comp_id / _struct_ref_seq_dif.details / _struct_site.pdbx_auth_asym_id / _struct_site.pdbx_auth_comp_id / _struct_site.pdbx_auth_seq_id

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: DNA-directed RNA polymerase subunit alpha
B: DNA-directed RNA polymerase subunit alpha
C: DNA-directed RNA polymerase subunit beta
D: DNA-DIRECTED RNA POLYMERASE SUBUNIT BETA'
E: DNA-directed RNA polymerase subunit omega
F: RNA polymerase sigma factor RpoD
G: DNA-directed RNA polymerase subunit alpha
H: DNA-directed RNA polymerase subunit alpha
I: DNA-directed RNA polymerase subunit beta
J: DNA-DIRECTED RNA POLYMERASE SUBUNIT BETA'
K: DNA-directed RNA polymerase subunit omega
L: RNA polymerase sigma factor RpoD
hetero molecules


Theoretical massNumber of molelcules
Total (without water)927,76926
Polymers925,60012
Non-polymers2,16914
Water00
1
A: DNA-directed RNA polymerase subunit alpha
B: DNA-directed RNA polymerase subunit alpha
C: DNA-directed RNA polymerase subunit beta
D: DNA-DIRECTED RNA POLYMERASE SUBUNIT BETA'
E: DNA-directed RNA polymerase subunit omega
F: RNA polymerase sigma factor RpoD
hetero molecules


Theoretical massNumber of molelcules
Total (without water)464,06015
Polymers462,8006
Non-polymers1,2609
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area42630 Å2
ΔGint-319 kcal/mol
Surface area167580 Å2
MethodPISA
2
G: DNA-directed RNA polymerase subunit alpha
H: DNA-directed RNA polymerase subunit alpha
I: DNA-directed RNA polymerase subunit beta
J: DNA-DIRECTED RNA POLYMERASE SUBUNIT BETA'
K: DNA-directed RNA polymerase subunit omega
L: RNA polymerase sigma factor RpoD
hetero molecules


Theoretical massNumber of molelcules
Total (without water)463,70911
Polymers462,8006
Non-polymers9095
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area41220 Å2
ΔGint-246 kcal/mol
Surface area170520 Å2
MethodPISA
Unit cell
Length a, b, c (Å)187.575, 206.164, 311.062
Angle α, β, γ (deg.)90.00, 90.00, 90.00
Int Tables number19
Space group name H-MP212121
Noncrystallographic symmetry (NCS)NCS oper:
IDCodeMatrixVector
1given(1), (1), (1)
2given(-0.423137, -0.006192, 0.906044), (0.06276, -0.997775, 0.022491), (0.903889, 0.06638, 0.422584)-67.48786, 221.84384, 36.36831
3given(-0.443194, 0.001027, 0.896425), (0.044547, -0.998739, 0.023168), (0.895318, 0.050201, 0.442589)-69.83331, 219.64449, 37.17186
4given(0.953395, -0.294807, -0.064243), (0.299596, 0.899711, 0.317431), (-0.035781, -0.321884, 0.946103)124.17278, 5.55655, -14.73211

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Components

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DNA-directed RNA polymerase subunit ... , 4 types, 10 molecules ABGHCIDJEK

#1: Protein
DNA-directed RNA polymerase subunit alpha / RNAP subunit alpha / RNA polymerase subunit alpha / Transcriptase subunit alpha


Mass: 36558.680 Da / Num. of mol.: 4
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Strain: K12 / DH10B / Gene: ECDH10B_3470, O3O_23125, rpoA / Production host: Escherichia coli (E. coli) / References: UniProt: K0BPQ3, DNA-directed RNA polymerase
#2: Protein DNA-directed RNA polymerase subunit beta / RNAP subunit beta / RNA polymerase subunit beta / Transcriptase subunit beta


Mass: 150820.875 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Strain: 2011C-3493 / Gene: ECDH1ME8569_3846, EcDH1_4008, O3K_23925, rpoB / Production host: Escherichia coli (E. coli)
References: UniProt: C9QV90, UniProt: A0A0E0Y7A2*PLUS, DNA-directed RNA polymerase
#3: Protein DNA-DIRECTED RNA POLYMERASE SUBUNIT BETA' / RNAP SUBUNIT BETA' / RNA POLYMERASE SUBUNIT BETA' / TRANSCRIPTASE SUBUNIT BETA'


Mass: 156537.031 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Strain: 2009EL-2071 / Gene: BWG_3647, O3O_01425, rpoC / Production host: Escherichia coli (E. coli) / References: UniProt: C5A0S8, DNA-directed RNA polymerase
#4: Protein DNA-directed RNA polymerase subunit omega / RNAP omega subunit / RNA polymerase omega subunit / Transcriptase subunit omega


Mass: 10118.352 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Strain: 2009EL-2071 / Gene: ECDH1ME8569_3534, EcDH1_0056, O3O_25075, rpoZ / Production host: Escherichia coli (E. coli) / References: UniProt: C9QUL2, DNA-directed RNA polymerase

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Protein , 1 types, 2 molecules FL

#5: Protein RNA polymerase sigma factor RpoD / Sigma-70


Mass: 72206.266 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Strain: K12 / Gene: alt, b3067, JW3039, rpoD / Production host: Escherichia coli (E. coli) / References: UniProt: P00579

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Non-polymers , 3 types, 14 molecules

#6: Chemical
ChemComp-SR / STRONTIUM ION


Mass: 87.620 Da / Num. of mol.: 8 / Source method: obtained synthetically / Formula: Sr
#7: Chemical ChemComp-G4P / GUANOSINE-5',3'-TETRAPHOSPHATE / guanosine tetraphosphate;ppGpp


Type: RNA linking / Mass: 603.160 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: C10H17N5O17P4
#8: Chemical
ChemComp-ZN / ZINC ION


Mass: 65.409 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: Zn

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 3.25 Å3/Da / Density % sol: 62.14 %
Crystal growTemperature: 298 K / Method: vapor diffusion, sitting drop / pH: 7
Details: 20% PEG400, 0.18M strontium chloride, 0.1M HEPES , pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 298K

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Data collection

DiffractionMean temperature: 100 K
Diffraction sourceSource: SYNCHROTRON / Site: NSLS / Beamline: X25 / Wavelength: 1.075 Å
DetectorType: ADSC QUANTUM 315 / Detector: CCD / Date: Jul 21, 2011
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 1.075 Å / Relative weight: 1
ReflectionResolution: 4.2→39.97 Å / Num. all: 88437 / Num. obs: 88171 / % possible obs: 99.7 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 13.2 %

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Processing

Software
NameVersionClassification
HKL-2000data collection
PHASERphasing
REFMAC5.7.0029refinement
HKL-2000data reduction
HKL-2000data scaling
RefinementMethod to determine structure: MOLECULAR REPLACEMENT / Resolution: 4.2→39.97 Å / Cor.coef. Fo:Fc: 0.929 / Cor.coef. Fo:Fc free: 0.884 / Cross valid method: THROUGHOUT / ESU R Free: 1.135 / Stereochemistry target values: MAXIMUM LIKELIHOOD
RfactorNum. reflection% reflectionSelection details
Rfree0.31802 4396 5 %RANDOM
Rwork0.24663 ---
obs0.25027 83775 99.55 %-
Solvent computationIon probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK
Displacement parametersBiso mean: 280.907 Å2
Baniso -1Baniso -2Baniso -3
1--0.78 Å20 Å20 Å2
2--0.67 Å20 Å2
3---0.11 Å2
Refinement stepCycle: LAST / Resolution: 4.2→39.97 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms58242 0 84 0 58326
Refine LS restraints
Refine-IDTypeDev idealDev ideal targetNumber
X-RAY DIFFRACTIONr_bond_refined_d0.010.01959183
X-RAY DIFFRACTIONr_angle_refined_deg0.9351.9879894
X-RAY DIFFRACTIONr_dihedral_angle_1_deg2.94457392
X-RAY DIFFRACTIONr_dihedral_angle_2_deg35.23824.1262785
X-RAY DIFFRACTIONr_dihedral_angle_3_deg13.8011511015
X-RAY DIFFRACTIONr_dihedral_angle_4_deg7.24915546
X-RAY DIFFRACTIONr_chiral_restr0.0570.29089
X-RAY DIFFRACTIONr_gen_planes_refined0.0040.02144252
LS refinement shellResolution: 4.2→4.308 Å / Total num. of bins used: 20
RfactorNum. reflection% reflection
Rfree0.418 308 -
Rwork0.393 6077 -
obs--99.95 %
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
19.1083-0.6881-0.73296.95333.05034.9597-0.0771.25610.0342-1.61830.18950.1146-0.6029-0.2769-0.11240.6372-0.0405-0.18080.73110.23770.2562-2.10690.986-45.739
26.9972-1.6781.90345.52080.81784.8752-0.1073-0.00010.0149-0.51080.0880.43280.7267-0.21950.01920.28460.0698-0.09230.608-0.02550.24898.49660.651-33.784
37.7098-4.1668-1.188412.86462.813612.4113-0.1105-0.3054-0.9485-0.1392-0.5752.1373-0.2389-0.95810.68540.2306-0.015-0.13640.9755-0.05721.047-18.38192.811-31.959
45.8745-2.78320.4377.70392.56713.1920.48910.59480.525-1.99860.24270.6936-0.2985-0.14-0.73171.42580.263-0.27160.97940.67781.5946-22.501125.374-39.386
52.1583-0.33880.15181.8406-0.39141.37350.0610.06840.3508-0.2116-0.0669-0.2892-0.37330.28210.00590.2352-0.1420.01970.59010.1040.419120.93692.984-11.285
62.31510.7402-0.25013.30291.60332.65590.1091-0.0840.55070.1692-0.13750.6276-0.1736-0.31750.02850.16650.08570.06530.58390.02630.7494-16.36191.70314.673
76.39440.1982-2.22392.7121-0.07362.8924-0.51340.1825-1.46110.14150.154-0.6670.20740.06020.35950.07770.0727-0.04020.6545-0.16771.178221.01444.409-12.133
811.63753.3773-0.63269.4461.52353.984-0.32660.1367-0.9205-0.0783-0.1558-0.10530.7411-0.09470.48240.5044-0.1384-0.0081.0484-0.08921.1173-33.92143.1462.764
92.3975-1.7572.25474.4796-3.25187.56070.0802-0.1772-0.65720.9498-0.2963-0.06730.6270.92930.21620.65570.21370.10260.89950.27220.788136.66852.59916.74
102.94160.72241.29397.0802-0.22235.761-0.2572-0.09320.16631.52790.3318-0.0833-0.2482-0.1695-0.07450.48640.2081-0.17790.98290.09450.603740.69680.85938.741
119.4845-0.3023-3.17785.4398-1.91482.0126-0.2995-0.13222.53220.98130.7549-2.08310.2929-0.0371-0.45542.69320.0507-1.54380.7719-0.57972.047656.835101.53758.56
121.66574.05492.11919.59556.76882.9704-0.7243-1.08941.33290.8076-0.49050.084-0.565-0.91521.21471.7789-0.075-1.70992.916-1.0022.39188.723121.6748.423
131.40611.31810.36962.5626-1.64044.4607-0.5411-0.7087-0.1564-1.21670.277-0.43360.2003-0.61720.26411.3677-0.33930.46362.1110.25573.167323.283129.99234.74
141.5392-0.27840.68711.7688-0.06382.74090.205-0.0642-0.30590.4327-0.0841-0.17420.7266-0.1091-0.12090.6495-0.067-0.15790.58480.05620.7251-1.6650.46252.396
155.52070.1662-3.83512.05052.77417.7938-0.50470.87360.3747-1.05740.27031.95570.4508-1.21290.23440.47670.0076-0.73631.09950.11061.3686-110.272124.23721.627
162.0407-2.1225-0.23558.92430.83128.5223-0.0110.51680.6089-0.65830.08111.1184-0.26140.1327-0.07020.44710.0676-0.43930.9370.37581.5663-105.439155.29535.07
177.9837-0.69281.490812.14199.95448.75710.2011.75550.0519-1.0530.1946-0.4402-0.71640.4129-0.39561.2005-0.2324-0.65531.11230.1750.8651-90.921122.96212.411
1814.483-8.6294-2.38098.46293.28622.74430.07721.4902-2.0187-0.4926-0.4472.3182-0.3581-0.31610.36991.5979-0.2161-0.80841.1161-0.46171.7298-93.09389.9236.768
191.28380.2464-0.17432.73311.49253.25720.1528-0.0383-0.07380.13950.12060.6980.5273-0.2439-0.27350.3906-0.0627-0.18010.46660.1830.7982-88.61124.77957.063
203.8025-1.30330.3433.0816-0.7412.0533-0.03960.6511-0.344-0.83330.0179-0.42820.37940.27940.02160.68030.0416-0.12060.7639-0.11910.6988-49.648127.62234.109
211.24780.204-0.40081.96420.00952.3271-0.0215-0.31080.5972-0.3963-0.10010.8111-0.5776-0.66080.12161.08550.2619-0.32081.08160.19881.4128-92.51173.04355.67
226.6053-1.44061.42628.0963-1.94813.7953-0.16650.1790.8889-0.66970.5881-0.438-0.5519-0.2-0.42161.4702-0.11780.11220.79820.1770.5311-52.538176.21212.541
235.06132.92444.02784.38943.18964.9047-0.0103-0.26240.42180.8147-0.2945-0.0434-0.74690.1460.30481.50540.0202-0.17730.36920.11410.928-73.01166.74283.238
245.23520.9912.81774.61750.04332.3485-0.16150.6486-0.28140.19120.39-0.2095-0.13690.3899-0.22860.76710.13070.04340.7564-0.19190.3021-52.198139.57395.31
2512.29985.51040.170812.8845-4.430710.1001-0.393-0.4451-2.14760.52710.083-1.1004-0.02330.10750.30990.86630.3182-0.31660.6066-0.20691.0358-40.652118.621117.891
2611.7324-8.16824.152414.8147-6.2052.73530.8304-0.51060.7955-0.8803-0.9931-0.92510.62520.23950.16271.88760.6587-0.57762.36820.29651.1928-28.39699.8372.61
272.7395-0.8452.42090.4959-1.41144.04560.2332-1.0983-0.3135-0.49530.04560.07761.4250.0355-0.27882.30160.2592-0.03331.9772-0.00752.5829-46.36890.35780.944
282.28880.55610.60722.06730.11532.0558-0.34160.28010.36630.00070.0804-0.0066-0.56420.1020.26130.849-0.0316-0.26560.6086-0.08340.571-23.481170.12363.507
Refinement TLS group
IDRefine-IDRefine TLS-IDAuth asym-IDAuth seq-ID
1X-RAY DIFFRACTION1A6 - 51
2X-RAY DIFFRACTION1A179 - 232
3X-RAY DIFFRACTION1A401
4X-RAY DIFFRACTION2A52 - 178
5X-RAY DIFFRACTION3B6 - 51
6X-RAY DIFFRACTION3B179 - 232
7X-RAY DIFFRACTION4B52 - 178
8X-RAY DIFFRACTION5C3 - 28
9X-RAY DIFFRACTION5C146 - 152
10X-RAY DIFFRACTION5C445 - 455
11X-RAY DIFFRACTION5C517 - 831
12X-RAY DIFFRACTION5C1057 - 1241
13X-RAY DIFFRACTION5C1402
14X-RAY DIFFRACTION5D501 - 790
15X-RAY DIFFRACTION6C1242 - 1320
16X-RAY DIFFRACTION6D343 - 500
17X-RAY DIFFRACTION6D791 - 930
18X-RAY DIFFRACTION6D1136 - 1153
19X-RAY DIFFRACTION6D1213 - 1317
20X-RAY DIFFRACTION6D1345 - 1376
21X-RAY DIFFRACTION6C1401
22X-RAY DIFFRACTION6D2003 - 2004
23X-RAY DIFFRACTION6E2 - 91
24X-RAY DIFFRACTION6D2001
25X-RAY DIFFRACTION6E101
26X-RAY DIFFRACTION6F520 - 529
27X-RAY DIFFRACTION7C832 - 891
28X-RAY DIFFRACTION7C912 - 1056
29X-RAY DIFFRACTION8C892 - 911
30X-RAY DIFFRACTION8F530 - 613
31X-RAY DIFFRACTION8F701
32X-RAY DIFFRACTION9C29 - 145
33X-RAY DIFFRACTION9C456 - 516
34X-RAY DIFFRACTION10C153 - 225
35X-RAY DIFFRACTION10C338 - 444
36X-RAY DIFFRACTION11C226 - 337
37X-RAY DIFFRACTION12D1154 - 1212
38X-RAY DIFFRACTION13D947 - 1126
39X-RAY DIFFRACTION14C1321 - 1342
40X-RAY DIFFRACTION14D10 - 342
41X-RAY DIFFRACTION14D1318 - 1344
42X-RAY DIFFRACTION14D2002
43X-RAY DIFFRACTION14F95 - 519
44X-RAY DIFFRACTION15G6 - 51
45X-RAY DIFFRACTION15G179 - 232
46X-RAY DIFFRACTION16G52 - 178
47X-RAY DIFFRACTION17H6 - 51
48X-RAY DIFFRACTION17H179 - 232
49X-RAY DIFFRACTION18H52 - 178
50X-RAY DIFFRACTION19I3 - 28
51X-RAY DIFFRACTION19I146 - 152
52X-RAY DIFFRACTION19I445 - 455
53X-RAY DIFFRACTION19I517 - 831
54X-RAY DIFFRACTION19I1057 - 1241
55X-RAY DIFFRACTION19J501 - 790
56X-RAY DIFFRACTION20I1242 - 1320
57X-RAY DIFFRACTION20J343 - 500
58X-RAY DIFFRACTION20J791 - 930
59X-RAY DIFFRACTION20J1136 - 1153
60X-RAY DIFFRACTION20J1213 - 1317
61X-RAY DIFFRACTION20J1345 - 1376
62X-RAY DIFFRACTION20I1401
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