Mass: 18.015 Da / Num. of mol.: 129 / Source method: isolated from a natural source / Formula: H2O
Nonpolymer details
HEN EGG WHITE LYSOZYME WAS CRYSTALLIZED WITH AN ORGANO-METALLIC RUTHENIUM(III) COMPOUND, CALLED ...HEN EGG WHITE LYSOZYME WAS CRYSTALLIZED WITH AN ORGANO-METALLIC RUTHENIUM(III) COMPOUND, CALLED AZIRU [SODIUM TRANS-(DIMETHYLSULFOXIDE)-PYRIDINE TETRACHLORO-RUTHENATE(III)]. AZIRU LOSES ALL ITS ORIGINAL LIGANDS, RU IS COORDINATED BY ATOMS OF HIS15, ASP87 AND WATER MOLECULES.
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 1.97 Å3/Da / Density % sol: 37.69 %
Crystal grow
Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 4.5 Details: 1.0-1.4 M NaCl, 0.050 M acetate buffer.Crystals were soaked in a saturated solution of AziRu containing 2.4 M NaCl in the same buffer. After a few hours, crystals changed their colour to ...Details: 1.0-1.4 M NaCl, 0.050 M acetate buffer.Crystals were soaked in a saturated solution of AziRu containing 2.4 M NaCl in the same buffer. After a few hours, crystals changed their colour to yellow and then green. In a few days they turned to black, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
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