PDB-4il2: Crystal structure of D-mannonate dehydratase (rspA) from E. coli ...

ID or keywords:

Entry

Database: PDB / ID: 4il2

Title

Crystal structure of D-mannonate dehydratase (rspA) from E. coli CFT073 (EFI TARGET EFI-501585)

Components

Starvation sensing protein rspA

Keywords

LYASE /

TIM-barrel /

Structural Genomics

Function / homology

Function and homology information

mannonate dehydratase /

mannonate dehydratase activity /

Lyases; Carbon-oxygen lyases; Hydro-lyases / amino acid catabolic process / carbohydrate catabolic process / magnesium ion binding
Similarity search - Function

Biological species

Escherichia coli (E. coli)

Method

X-RAY DIFFRACTION /

SYNCHROTRON /

MOLECULAR REPLACEMENT / Resolution: 1.95 Å

Authors

Lukk, T. / Wichelecki, D. / Imker, H.J. / Gerlt, J.A. / Nair, S.K.

Citation

Journal: To be Published
Title: Mannonate degradation pathway in E. coli CFT073
Authors: Wichelecki, S. / Lukk, T. / Imker, H.J. / Nair, S.K. / Gerlt, J.A.

History

Deposition	Dec 28, 2012	Deposition site: RCSB / Processing site: RCSB
Revision 1.0	Feb 20, 2013	Provider: repository / Type: Initial release
Revision 1.1	Feb 28, 2024	Group: Data collection / Database references / Derived calculations Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_struct_conn_angle / struct_conn / struct_ref_seq_dif / struct_site Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession ..._database_2.pdbx_DOI / _database_2.pdbx_database_accession / _pdbx_struct_conn_angle.ptnr1_auth_asym_id / _pdbx_struct_conn_angle.ptnr1_auth_comp_id / _pdbx_struct_conn_angle.ptnr1_auth_seq_id / _pdbx_struct_conn_angle.ptnr1_label_asym_id / _pdbx_struct_conn_angle.ptnr1_label_atom_id / _pdbx_struct_conn_angle.ptnr1_label_comp_id / _pdbx_struct_conn_angle.ptnr1_label_seq_id / _pdbx_struct_conn_angle.ptnr2_auth_asym_id / _pdbx_struct_conn_angle.ptnr2_label_asym_id / _pdbx_struct_conn_angle.ptnr3_auth_asym_id / _pdbx_struct_conn_angle.ptnr3_auth_comp_id / _pdbx_struct_conn_angle.ptnr3_auth_seq_id / _pdbx_struct_conn_angle.ptnr3_label_asym_id / _pdbx_struct_conn_angle.ptnr3_label_atom_id / _pdbx_struct_conn_angle.ptnr3_label_comp_id / _pdbx_struct_conn_angle.ptnr3_label_seq_id / _pdbx_struct_conn_angle.value / _struct_conn.pdbx_dist_value / _struct_conn.ptnr1_auth_asym_id / _struct_conn.ptnr1_auth_comp_id / _struct_conn.ptnr1_auth_seq_id / _struct_conn.ptnr1_label_asym_id / _struct_conn.ptnr1_label_atom_id / _struct_conn.ptnr1_label_comp_id / _struct_conn.ptnr1_label_seq_id / _struct_conn.ptnr2_auth_asym_id / _struct_conn.ptnr2_auth_comp_id / _struct_conn.ptnr2_auth_seq_id / _struct_conn.ptnr2_label_asym_id / _struct_conn.ptnr2_label_atom_id / _struct_conn.ptnr2_label_comp_id / _struct_ref_seq_dif.details / _struct_site.pdbx_auth_asym_id / _struct_site.pdbx_auth_comp_id / _struct_site.pdbx_auth_seq_id

Structure viewer	Molecule: MolmilJmol/JSmol

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Download

PDBx/mmCIF format	4il2.cif.gz	612.2 KB	Display	PDBx/mmCIF format
PDB format	pdb4il2.ent.gz	505.3 KB	Display	PDB format
PDBx/mmJSON format	4il2.json.gz		Tree view	PDBx/mmJSON format
Others	Other downloads

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Validation report

Arichive directory	https://data.pdbj.org/pub/pdb/validation_reports/il/4il2 ftp://data.pdbj.org/pub/pdb/validation_reports/il/4il2	HTTPS FTP

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Related structure data

Similar structure data	4kt2-d 4kpl-d 3vcn-2 4ihc-d 2qjj-3 4f4r-1 3rgt-3 4gme-1 3ow1-d 3v3w-1 Search similar-shape structures by Omokage search (details) Search similar-shape structures by Omokage search (details) Search similar-shape structures by Omokage search (details) Similarity search - Function & homologyF&H Search Similarity search - FunctionF&H Search Similarity search - HomologyF&H Search
Other databases	TargetTrack: EFI-501585

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Links

PDB pages	PDBj / wwPDB / NCBI
Related items in Molecule of the Month	#50 - Feb 2004 Glycolytic Enzymes similarity (2)

Deposited unit

A: Starvation sensing protein rspA

B: Starvation sensing protein rspA

C: Starvation sensing protein rspA

D: Starvation sensing protein rspA

hetero molecules

196 kDa, 4 polymers
Search similar-shape structures of this assembly by Omokage search (details)

1

A: Starvation sensing protein rspA

B: Starvation sensing protein rspA

hetero molecules

A: Starvation sensing protein rspA

B: Starvation sensing protein rspA

hetero molecules

A: Starvation sensing protein rspA

B: Starvation sensing protein rspA

hetero molecules

A: Starvation sensing protein rspA

B: Starvation sensing protein rspA

hetero molecules

defined by author&software
392 kDa, 8 polymers
Search similar-shape structures of this assembly by Omokage search (details)

2

C: Starvation sensing protein rspA

D: Starvation sensing protein rspA

hetero molecules

C: Starvation sensing protein rspA

D: Starvation sensing protein rspA

hetero molecules

C: Starvation sensing protein rspA

D: Starvation sensing protein rspA

hetero molecules

C: Starvation sensing protein rspA

D: Starvation sensing protein rspA

hetero molecules

defined by author&software
392 kDa, 8 polymers
Search similar-shape structures of this assembly by Omokage search (details)

Unit cell

Length a, b, c (Å)	123.780, 123.780, 112.210
Angle α, β, γ (deg.)	90.00, 90.00, 90.00
Int Tables number	75
Space group name H-M	P4

Components on special symmetry positions

ID	Model	Components
1	1	B-631- HOH
2	1	B-725- HOH

#1: Protein	Starvation sensing protein rspA Mass: 48921.570 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli (E. coli) / Strain: CFT073 / Gene: c1971, rspA / Plasmid: pUC18 / Production host: Escherichia coli (E. coli) / Strain (production host): BL21(DE3) / References: UniProt: Q8FHC7
#2: Chemical	ChemComp-MG / MAGNESIUM ION Mass: 24.305 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: Mg
#3: Water	ChemComp-HOH / water / Water Mass: 18.015 Da / Num. of mol.: 472 / Source method: isolated from a natural source / Formula: H₂O

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Experiment

Experiment	Method: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

Crystal	Density Matthews: 2.2 Å³/Da / Density % sol: 44 %
Crystal grow	Temperature: 282 K / Method: vapor diffusion, sitting drop / pH: 7.5 Details: Protein solution was at 10 mg/mL containing 20 mM Tris (pH 7.5), 100 mM NaCl and 50 mM imidazole. Mother liqueur contained 25% PEG 1,500 and 20% v/v glycerol, vapor diffusion, sitting drop, temperature 282K

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Data collection

Diffraction

Mean temperature: 100 K

Diffraction source

Source:

SYNCHROTRON / Site:

APS

/ Beamline: 21-ID-F / Wavelength: 0.97872 Å

Detector

Type: RAYONIX MX-225 / Detector: CCD / Date: Mar 23, 2012

Radiation

Monochromator: C(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray

Radiation wavelength

Wavelength: 0.97872 Å / Relative weight: 1

Reflection

Number: 627522 / R_merge(I) obs: 0.116 / D res high: 1.95 Å / Num. obs: 122958 / % possible obs: 99.9

Diffraction reflection shell

Highest resolution (Å)	Lowest resolution (Å)	Num. obs	% possible obs (%)	ID	R_merge(I) obs
8.72	30	1401	97	1	0.031
6.17	8.72	2563	100	1	0.038
5.03	6.17	3298	100	1	0.045
4.36	5.03	3908	100	1	0.042
3.9	4.36	4380	100	1	0.045
3.56	3.9	4849	100	1	0.051
3.3	3.56	5277	100	1	0.061
3.08	3.3	5657	100	1	0.076
2.91	3.08	6053	100	1	0.095
2.76	2.91	6312	100	1	0.118
2.63	2.76	6690	99.9	1	0.148
2.52	2.63	6976	100	1	0.168
2.42	2.52	7247	100	1	0.199
2.33	2.42	7586	100	1	0.227
2.25	2.33	7833	99.9	1	0.254
2.18	2.25	8083	99.9	1	0.303
2.12	2.18	8323	99.9	1	0.352
2.06	2.12	8603	99.9	1	0.441
2	2.06	8850	100	1	0.537

Reflection

Resolution: 1.95→30 Å / Num. all: 123046 / Num. obs: 122978 / % possible obs: 99.9 % / Observed criterion σ(F): 0 / Observed criterion σ(I): -3 / R_sym value: 0.116 / Net I/σ(I): 12.82

Reflection shell

Resolution (Å)	R_merge(I) obs	Mean I/σ(I) obs	Diffraction-ID	% possible all
1.95-2	0.663	2.56	1	100
2-2.06	0.537	3.16	1	100
2.06-2.12	0.441	3.85	1	99.9
2.12-2.18	0.352	4.84	1	99.9
2.18-2.25	0.303	5.62	1	99.9
2.25-2.33	0.254	6.72	1	99.9
2.33-2.42	0.227	7.44	1	100
2.42-2.52	0.199	8.48	1	100
2.52-2.63	0.168	9.93	1	100
2.63-2.76	0.148	11.18	1	99.9
2.76-2.91	0.118	13.66	1	100
2.91-3.08	0.095	16.61	1	100
3.08-3.3	0.076	20.09	1	100
3.3-3.56	0.061	24.6	1	100
3.56-3.9	0.051	28.96	1	100
3.9-4.36	0.045	32.05	1	100
4.36-5.03	0.042	34.07	1	100
5.03-6.17	0.045	31.43	1	100
6.17-8.72	0.038	34.26	1	100
8.72-30	0.031	39.74	1	97

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Processing

Software

Name	Version	Classification
XSCALE		data scaling
PHENIX	1.7.3_928	refinement
PDB_EXTRACT	3.11	data extraction
HKL-3000		data collection
XDS		data reduction
PHASER		phasing

Refinement

Method to determine structure:

MOLECULAR REPLACEMENT / Resolution: 1.95→29.831 Å / Occupancy max: 1 / Occupancy min: 1 / SU ML: 0.21 / σ(F): 0 / Phase error: 19.46 / Stereochemistry target values: ML

	R_factor	Num. reflection	% reflection
R_free	0.2093	6075	4.94 %
R_work	0.1778	-	-
obs	0.1794	122978	99.99 %
all	-	122977	-

Solvent computation

Shrinkage radii: 0.98 Å / VDW probe radii: 1.2 Å / Solvent model: FLAT BULK SOLVENT MODEL / B_sol: 29.985 Å² / k_sol: 0.4 e/Å³

Displacement parameters

	B_aniso -1	B_aniso -2	B_aniso -3
1-	-0.0674 Å²	-0 Å²	0 Å²
2-	-	-0.0674 Å²	-0 Å²
3-	-	-	0.1349 Å²

Refinement step

Cycle: LAST / Resolution: 1.95→29.831 Å

	Protein	Nucleic acid	Ligand	Solvent	Total
Num. atoms	12364	0	4	472	12840

Refine LS restraints

Refine-ID	Type	Dev ideal	Number
X-RAY DIFFRACTION	f_bond_d	0.01	12720
X-RAY DIFFRACTION	f_angle_d	1.475	17266
X-RAY DIFFRACTION	f_dihedral_angle_d	16.966	4593
X-RAY DIFFRACTION	f_chiral_restr	0.093	1807
X-RAY DIFFRACTION	f_plane_restr	0.007	2237

LS refinement shell

Resolution (Å)	R_factor R_free	Num. reflection R_free	R_factor R_work	Num. reflection R_work	Refine-ID	% reflection obs (%)
1.95-1.9722	0.264	225	0.1972	3867	X-RAY DIFFRACTION	100
1.9722-1.9954	0.2276	209	0.1752	3850	X-RAY DIFFRACTION	100
1.9954-2.0197	0.2306	198	0.1689	3914	X-RAY DIFFRACTION	100
2.0197-2.0453	0.2111	209	0.1585	3870	X-RAY DIFFRACTION	100
2.0453-2.0722	0.2199	234	0.1658	3840	X-RAY DIFFRACTION	100
2.0722-2.1006	0.1853	188	0.1531	3904	X-RAY DIFFRACTION	100
2.1006-2.1306	0.1835	202	0.1543	3884	X-RAY DIFFRACTION	100
2.1306-2.1624	0.2261	196	0.165	3883	X-RAY DIFFRACTION	100
2.1624-2.1961	0.21	188	0.1586	3877	X-RAY DIFFRACTION	100
2.1961-2.2321	0.1886	198	0.1637	3913	X-RAY DIFFRACTION	100
2.2321-2.2706	0.2076	206	0.167	3874	X-RAY DIFFRACTION	100
2.2706-2.3119	0.2289	202	0.1698	3903	X-RAY DIFFRACTION	100
2.3119-2.3563	0.2137	202	0.1783	3867	X-RAY DIFFRACTION	100
2.3563-2.4044	0.2231	198	0.1777	3863	X-RAY DIFFRACTION	100
2.4044-2.4567	0.2142	200	0.176	3923	X-RAY DIFFRACTION	100
2.4567-2.5138	0.2217	194	0.1839	3888	X-RAY DIFFRACTION	100
2.5138-2.5766	0.2277	220	0.1886	3891	X-RAY DIFFRACTION	100
2.5766-2.6463	0.2217	201	0.1897	3885	X-RAY DIFFRACTION	100
2.6463-2.7241	0.2207	206	0.1919	3854	X-RAY DIFFRACTION	100
2.7241-2.8119	0.2087	208	0.1854	3932	X-RAY DIFFRACTION	100
2.8119-2.9123	0.2085	183	0.1809	3897	X-RAY DIFFRACTION	100
2.9123-3.0288	0.2185	197	0.1835	3913	X-RAY DIFFRACTION	100
3.0288-3.1666	0.2276	185	0.1897	3901	X-RAY DIFFRACTION	100
3.1666-3.3333	0.2493	205	0.2009	3910	X-RAY DIFFRACTION	100
3.3333-3.5418	0.1984	221	0.186	3900	X-RAY DIFFRACTION	100
3.5418-3.8148	0.2003	202	0.1726	3885	X-RAY DIFFRACTION	100
3.8148-4.1977	0.1894	219	0.1646	3922	X-RAY DIFFRACTION	100
4.1977-4.803	0.1712	189	0.1581	3926	X-RAY DIFFRACTION	100
4.803-6.043	0.2034	199	0.1946	3949	X-RAY DIFFRACTION	100
6.043-29.8349	0.1923	191	0.1888	4018	X-RAY DIFFRACTION	100

Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

ID	L11 (°²)	L12 (°²)	L13 (°²)	L22 (°²)	L23 (°²)	L33 (°²)	S11 (Å °)	S12 (Å °)	S13 (Å °)	S21 (Å °)	S22 (Å °)	S23 (Å °)	S31 (Å °)	S32 (Å °)	S33 (Å °)	T11 (Å²)	T12 (Å²)	T13 (Å²)	T22 (Å²)	T23 (Å²)	T33 (Å²)	Origin x (Å)	Origin y (Å)	Origin z (Å)
1	0.3383	0.1364	0.1221	0.4883	-0.1498	0.3739	0.013	-0.1276	-0.0202	0.098	-0.0098	-0.1333	-0.0162	0.067	0.0127	0.1115	0.0157	-0.0343	0.1468	0.0296	0.137	-25.9591	45.2978	-24.5235
2	0.0763	0.0765	0.1395	-0.0087	0.0269	-0.0173	-0.0001	-0.0702	-0.1314	0.304	0.0046	-0.1896	0.1863	0.1494	0.018	0.3131	-0.0052	-0.0115	0.257	0.0307	0.1678	-41.4482	41.5663	-2.9622
3	0.1411	-0.1622	-0.0227	0.0943	-0.0044	0.1321	0.0851	-0.0788	0.1614	0.1272	-0.1318	0.0537	0.0075	0.0277	-0.0219	0.2355	0.0301	0.0181	0.2677	0.034	0.1126	-49.369	47.8266	2.8804
4	0.1927	-0.0374	-0.1271	0.1661	-0.1928	0.4491	-0.0031	-0.0871	0.0023	0.1503	0.102	-0.0896	0.0221	0.1764	-0.0239	0.2276	0.0216	-0.0027	0.2397	0.0036	0.135	-48.4174	51.6439	-4.4327
5	0.4854	-0.2405	-0.3129	0.9966	-0.1166	0.3069	-0.0407	-0.1614	-0.0042	0.1155	0.0277	-0.1348	0.0095	0.0907	0.0145	0.1235	0.0007	-0.0263	0.1303	0.0176	0.0963	-36.36	52.5177	-17.099
6	0.2558	-0.2739	0.1181	0.446	0.0934	0.2585	-0.0155	-0.1134	-0.18	0.0843	-0.0246	-0.1407	0.0631	0.1174	0.0146	0.1935	0.033	-0.0453	0.1965	0.0371	0.152	-30.0653	36.1817	-15.7474
7	0.5896	0.074	0.1501	0.3338	0.0971	0.4153	-0.0186	-0.1435	-0.152	0.1502	-0.0171	-0.0161	0.106	0.0008	0.0216	0.1209	0.0207	-0.0007	0.099	0.0337	0.1145	-43.8823	23.7808	-29.3501
8	0.2909	-0.0739	-0.2192	0.0696	0.0403	0.0436	-0.0465	0.0264	-0.1514	-0.0917	0.0083	-0.0374	0.2889	-0.1901	0.0158	0.0971	-0.0125	0.0125	0.082	0.0096	0.1305	-62.8016	21.6728	-41.7497
9	0.1963	-0.1175	-0.0769	0.6995	-0.1195	0.4975	0.0399	0.1422	-0.1518	-0.0752	-0.0282	-0.1381	0.1288	0.148	0.016	0.0838	0.0128	0.0061	0.0889	0.007	0.0936	-44.9714	42.115	-59.3621
10	0.9774	-0.3124	0.076	0.8574	-0.7519	0.2816	0.0461	0.0105	-0.1065	-0.0116	-0.0279	-0.0266	0.1107	0.0302	0.0092	0.0528	-0.0041	0.007	0.0576	-0.0033	0.0515	-53.6523	35.8915	-43.7774
11	0.8312	-0.0112	-0.0279	0.2033	0.2478	0.297	-0.0423	0.0235	-0.1611	-0.0555	0.0315	-0.0307	0.0897	0.0014	0.0203	0.0668	0.0121	0.0092	0.0815	-0.0054	0.0852	-37.6253	29.0285	-45.0729
12	0.4444	0.0294	0.0242	0.4704	-0.0409	0.6985	-0.0083	0.0691	0.0873	-0.0586	0.0034	0.0441	-0.0689	-0.0193	-0.0003	0.0905	0.0154	0.0104	0.0588	0.0131	0.1145	-16.5001	34.2456	-76.0673
13	0.2647	-0.141	-0.0576	0.0065	0.062	-0.0018	0.0645	-0.3718	0.1319	0.0905	-0.0585	-0.0196	-0.0582	0.1805	0.0256	0.077	0.0028	0.0132	0.0573	0.0034	0.0804	-2.1758	18.8707	-51.3131
14	0.7906	-0.193	0.4562	1.0656	-0.2214	0.7944	-0.0186	0.0491	0.0147	0.0146	0.0132	0.0244	-0.0641	-0.0068	0.0089	0.0188	-0.0029	0.0115	0.0208	0.0096	0.0309	-7.5895	16.9835	-70.2686
15	0.4951	-0.3149	0.0529	0.2987	-0.2522	0.4139	0.0311	-0.027	0.0992	0.0956	0.008	0.0668	-0.11	-0.0276	-0.0118	0.065	0.009	0.0046	0.0749	0.0138	0.1012	-21.7017	30.5146	-67.8635
16	0.465	0.3192	-0.0183	0.4904	0.1532	0.5133	0.018	-0.0582	0.169	-0.0134	-0.0091	0.0326	-0.101	-0.0387	-0.0111	0.1133	-0.0149	0.0132	0.0246	0.0244	0.0922	-9.4668	39.7225	-70.2239
17	0.1227	0.1035	0.2676	0.4377	-0.1441	0.3903	-0.0062	0.0136	0.0028	-0.0346	0.0377	0.1639	-0.0228	-0.1349	-0.0239	0.093	0.0056	-0.0284	0.1487	0.0211	0.1316	-41.1596	8.8303	-83.9907
18	0.2197	0.0944	-0.205	-0.022	0.064	0.1112	0.0502	0.2027	-0.0228	-0.2626	-0.0925	0.0849	-0.008	-0.1292	-0.0087	0.2895	0.0371	-0.0673	0.2923	0.0251	0.1658	-30.6708	14.3819	-108.9523
19	0.3624	0.0461	-0.028	0.2415	0.2109	0.3194	-0.0996	0.1048	0.024	-0.2146	0.1866	0.1848	0.0834	-0.1059	-0.0025	0.2626	0.0281	-0.0436	0.3061	-0.0034	0.1698	-28.7839	-2.4399	-112.5524
20	0.0921	-0.0626	0.0725	-0.0189	-0.0745	0.139	0.0053	0.1482	0.0122	-0.3416	-0.0205	-0.2211	0.0781	0.1187	0.0192	0.2816	0.0142	-0.0368	0.3651	0.0132	0.1484	-18.5585	-2.7074	-118.4444
21	0.7379	-0.189	-0.0194	0.4838	0.3685	0.1785	0.0301	0.1819	-0.0133	-0.1263	-0.0075	0.0358	0.004	-0.0275	-0.0122	0.1713	0.0014	-0.0286	0.1544	0.019	0.0899	-20.5995	5.7289	-99.6581
22	0.0627	-0.0418	-0.0481	0.0926	0.0267	0.1216	-0.034	0.2636	0.1384	-0.2327	-0.0044	0.1273	-0.1551	-0.1814	0.0007	0.2366	0.0326	-0.0626	0.2725	0.0224	0.2147	-37.1434	15.5727	-104.8931
23	0.7083	0.0376	0.3004	0.6928	-0.2109	0.5385	0.0157	0.0248	-0.0079	-0.1438	-0.0267	0.2054	-0.0751	-0.1382	0.0284	0.1246	-0.0097	-0.0385	0.1872	0.0052	0.0989	-40.8417	0.8011	-99.7005

Refinement TLS group

ID	Refine-ID	Refine TLS-ID	Selection details
1	X-RAY DIFFRACTION	1	chain 'A' and (resseq 13:151)
2	X-RAY DIFFRACTION	2	chain 'A' and (resseq 152:195)
3	X-RAY DIFFRACTION	3	chain 'A' and (resseq 196:213)
4	X-RAY DIFFRACTION	4	chain 'A' and (resseq 214:243)
5	X-RAY DIFFRACTION	5	chain 'A' and (resseq 244:376)
6	X-RAY DIFFRACTION	6	chain 'A' and (resseq 377:415)
7	X-RAY DIFFRACTION	7	chain 'B' and (resseq 12:111)
8	X-RAY DIFFRACTION	8	chain 'B' and (resseq 112:130)
9	X-RAY DIFFRACTION	9	chain 'B' and (resseq 131:243)
10	X-RAY DIFFRACTION	10	chain 'B' and (resseq 244:376)
11	X-RAY DIFFRACTION	11	chain 'B' and (resseq 377:415)
12	X-RAY DIFFRACTION	12	chain 'C' and (resseq 12:195)
13	X-RAY DIFFRACTION	13	chain 'C' and (resseq 196:214)
14	X-RAY DIFFRACTION	14	chain 'C' and (resseq 215:318)
15	X-RAY DIFFRACTION	15	chain 'C' and (resseq 319:376)
16	X-RAY DIFFRACTION	16	chain 'C' and (resseq 377:415)
17	X-RAY DIFFRACTION	17	chain 'D' and (resseq 13:111)
18	X-RAY DIFFRACTION	18	chain 'D' and (resseq 112:151)
19	X-RAY DIFFRACTION	19	chain 'D' and (resseq 152:195)
20	X-RAY DIFFRACTION	20	chain 'D' and (resseq 196:213)
21	X-RAY DIFFRACTION	21	chain 'D' and (resseq 214:344)
22	X-RAY DIFFRACTION	22	chain 'D' and (resseq 345:376)
23	X-RAY DIFFRACTION	23	chain 'D' and (resseq 377:415)

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