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Open data
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Basic information
Entry | Database: PDB / ID: 4h7b | ||||||
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Title | Crystal Structure of Staphylococcal nuclease mutant I72V/V99L | ||||||
![]() | Thermonuclease | ||||||
![]() | HYDROLASE / DNA hydrolase / RNA hydrolase / endonuclease | ||||||
Function / homology | ![]() endonuclease activity, active with either ribo- or deoxyribonucleic acids and producing 3'-phosphomonoesters / micrococcal nuclease / nucleic acid binding / extracellular region / membrane / metal ion binding Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Sanders, J.M. / Janowska, K. / Sakon, J. / Stites, W.E. | ||||||
![]() | ![]() Title: Hydrophobic core mutants of Staphylococcal nuclease Authors: Sanders, J.M. / Janowska, K. / Sakon, J. / Stites, W.E. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 40.9 KB | Display | ![]() |
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PDB format | ![]() | 28.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 427.2 KB | Display | ![]() |
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Full document | ![]() | 429.5 KB | Display | |
Data in XML | ![]() | 8.2 KB | Display | |
Data in CIF | ![]() | 10.7 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 2f0dC ![]() 2f0eC ![]() 2f0fC ![]() 2f0gC ![]() 2f0hC ![]() 2f0iC ![]() 2f0jC ![]() 2f0kC ![]() 2f0lC ![]() 2f0mC ![]() 2f0nC ![]() 2f0oC ![]() 2f0pC ![]() 2f0qC ![]() 2f0sC ![]() 2f0tC ![]() 2f0uC ![]() 2f0vC ![]() 2f0wC ![]() 4i65C ![]() 4k8iC ![]() 4k8jC C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 16843.330 Da / Num. of mol.: 1 / Fragment: UNP Residues 83-231 / Mutation: I72V, V99L Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
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#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.14 Å3/Da / Density % sol: 42.39 % |
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Crystal grow | Temperature: 277 K / Method: vapor diffusion, hanging drop / pH: 7 Details: 25mM Sodium Phosphate buffer, 35-60% MPD, Vapor Diffusion, Hanging Drop, Temperature 277K, pH 7.0, VAPOR DIFFUSION, HANGING DROP |
-Data collection
Diffraction | Mean temperature: 293 K |
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Diffraction source | Source: ![]() |
Detector | Type: RIGAKU SATURN 92 / Detector: CCD |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 1.6→21.22 Å / Num. obs: 14927 |
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Processing
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Refinement | Method to determine structure: ![]()
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.4 Å / Solvent model: MASK | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 37.763 Å2
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Refinement step | Cycle: LAST / Resolution: 1.6→21.22 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.6→1.642 Å / Total num. of bins used: 20
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