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- PDB-4gyp: Crystal structure of the heterotetrameric complex of GlucD and Gl... -

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Basic information

Entry
Database: PDB / ID: 4gyp
TitleCrystal structure of the heterotetrameric complex of GlucD and GlucDRP from E. coli K-12 MG1655 (EFI TARGET EFI-506058)
Components
  • Glucarate dehydratase
  • Glucarate dehydratase-related protein
KeywordsLYASE/LYASE / TIM-BARREL / LYASE-LYASE complex / Structural genomics / Enzyme Function Initiative (EFI)
Function / homology
Function and homology information


: / glucarate dehydratase activity / D-glucarate catabolic process / glucarate dehydratase / Lyases; Carbon-oxygen lyases; Hydro-lyases / amino acid catabolic process / magnesium ion binding / metal ion binding
Similarity search - Function
Glucarate dehydratase / D-Glucarate dehydratase-like / Mandelate racemase / muconate lactonizing enzyme family signature 1. / Mandelate racemase/muconate lactonizing enzyme, conserved site / Mandelate racemase DgoD-like / Mandelate racemase/muconate lactonizing enzyme, N-terminal domain / Mandelate racemase / muconate lactonizing enzyme, N-terminal domain / Mandelate racemase/muconate lactonizing enzyme, C-terminal / Mandelate racemase / muconate lactonizing enzyme, C-terminal domain / Enolase C-terminal domain-like ...Glucarate dehydratase / D-Glucarate dehydratase-like / Mandelate racemase / muconate lactonizing enzyme family signature 1. / Mandelate racemase/muconate lactonizing enzyme, conserved site / Mandelate racemase DgoD-like / Mandelate racemase/muconate lactonizing enzyme, N-terminal domain / Mandelate racemase / muconate lactonizing enzyme, N-terminal domain / Mandelate racemase/muconate lactonizing enzyme, C-terminal / Mandelate racemase / muconate lactonizing enzyme, C-terminal domain / Enolase C-terminal domain-like / Enolase C-terminal domain-like / Enolase-like C-terminal domain / Enolase-like, N-terminal domain / Enolase-like, N-terminal / Enolase-like, C-terminal domain superfamily / Enolase-like; domain 1 / TIM Barrel / Alpha-Beta Barrel / 2-Layer Sandwich / Alpha Beta
Similarity search - Domain/homology
CITRIC ACID / DI(HYDROXYETHYL)ETHER / Glucarate dehydratase / Glucarate dehydratase-related protein
Similarity search - Component
Biological speciesEscherichia coli (E. coli)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / molecular replacement / Resolution: 2.1 Å
AuthorsLukk, T. / Ghasempur, S. / Imker, H.J. / Gerlt, J.A. / Nair, S.K. / Enzyme Function Initiative (EFI)
CitationJournal: to be published
Title: Glucarate dehydratase and its related protein from Escherichia coli form a heterotetrameric complex.
Authors: Lukk, T. / Ghasempur, S. / Imker, H.J. / Nair, S.K. / Gerlt, J.A.
History
DepositionSep 5, 2012Deposition site: RCSB / Processing site: RCSB
Revision 1.0Sep 12, 2012Provider: repository / Type: Initial release
Revision 1.1Sep 19, 2012Group: Structure summary
Revision 1.2Sep 13, 2023Group: Data collection / Database references ...Data collection / Database references / Derived calculations / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model / pdbx_struct_conn_angle / struct_conn / struct_ref_seq_dif / struct_site
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession ..._database_2.pdbx_DOI / _database_2.pdbx_database_accession / _pdbx_struct_conn_angle.ptnr1_auth_comp_id / _pdbx_struct_conn_angle.ptnr1_auth_seq_id / _pdbx_struct_conn_angle.ptnr1_label_atom_id / _pdbx_struct_conn_angle.ptnr1_label_comp_id / _pdbx_struct_conn_angle.ptnr1_label_seq_id / _pdbx_struct_conn_angle.ptnr3_auth_comp_id / _pdbx_struct_conn_angle.ptnr3_auth_seq_id / _pdbx_struct_conn_angle.ptnr3_label_atom_id / _pdbx_struct_conn_angle.ptnr3_label_comp_id / _pdbx_struct_conn_angle.ptnr3_label_seq_id / _pdbx_struct_conn_angle.value / _struct_conn.pdbx_dist_value / _struct_conn.ptnr1_auth_asym_id / _struct_conn.ptnr1_auth_comp_id / _struct_conn.ptnr1_auth_seq_id / _struct_conn.ptnr1_label_asym_id / _struct_conn.ptnr1_label_atom_id / _struct_conn.ptnr1_label_comp_id / _struct_conn.ptnr1_label_seq_id / _struct_conn.ptnr2_auth_asym_id / _struct_conn.ptnr2_label_asym_id / _struct_ref_seq_dif.details / _struct_site.pdbx_auth_asym_id / _struct_site.pdbx_auth_comp_id / _struct_site.pdbx_auth_seq_id

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Glucarate dehydratase
B: Glucarate dehydratase
C: Glucarate dehydratase-related protein
D: Glucarate dehydratase-related protein
hetero molecules


Theoretical massNumber of molelcules
Total (without water)200,78818
Polymers199,1944
Non-polymers1,59414
Water26,1221450
1


  • Idetical with deposited unit
  • defined by author
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Unit cell
Length a, b, c (Å)155.470, 113.020, 128.590
Angle α, β, γ (deg.)90.000, 105.890, 90.000
Int Tables number5
Space group name H-MC121

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Components

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Protein , 2 types, 4 molecules ABCD

#1: Protein Glucarate dehydratase / GDH / GlucD


Mass: 49196.855 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Strain: K12 / Gene: b2787, gudD, JW2758, ygcX / Plasmid: pUC18 / Production host: Escherichia coli (E. coli) / Strain (production host): BL21(DE3) / References: UniProt: P0AES2, glucarate dehydratase
#2: Protein Glucarate dehydratase-related protein / GDH-RP / GlucDRP


Mass: 50399.965 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Strain: K12 / Gene: b2788, gudX, JW2759, ygcY / Plasmid: pUC18 / Production host: Escherichia coli (E. coli) / Strain (production host): BL21(DE3)
References: UniProt: Q46915, Lyases; Carbon-oxygen lyases; Hydro-lyases

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Non-polymers , 7 types, 1464 molecules

#3: Chemical ChemComp-MG / MAGNESIUM ION


Mass: 24.305 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: Mg
#4: Chemical ChemComp-SO4 / SULFATE ION


Mass: 96.063 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: SO4
#5: Chemical
ChemComp-GOL / GLYCEROL / GLYCERIN / PROPANE-1,2,3-TRIOL


Mass: 92.094 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: C3H8O3
#6: Chemical ChemComp-PEG / DI(HYDROXYETHYL)ETHER


Mass: 106.120 Da / Num. of mol.: 3 / Source method: obtained synthetically / Formula: C4H10O3
#7: Chemical ChemComp-CIT / CITRIC ACID


Mass: 192.124 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: C6H8O7
#8: Chemical ChemComp-P6G / HEXAETHYLENE GLYCOL / POLYETHYLENE GLYCOL PEG400


Mass: 282.331 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C12H26O7 / Comment: precipitant*YM
#9: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 1450 / Source method: isolated from a natural source / Formula: H2O

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.73 Å3/Da / Density % sol: 54.9 %
Crystal growTemperature: 282 K / Method: vapor diffusion, sitting drop
Details: Protein solution was at 12.5 mg/mL containing 20 mM Tris (pH 7.5), 100 mM NaCl, 10 mM EDTA and 10 mM bME. Mother liqueur contained 0.17 M Ammonium acetate, 0.085 M Na-citrate tribasic, ...Details: Protein solution was at 12.5 mg/mL containing 20 mM Tris (pH 7.5), 100 mM NaCl, 10 mM EDTA and 10 mM bME. Mother liqueur contained 0.17 M Ammonium acetate, 0.085 M Na-citrate tribasic, dihydrate (pH 5.6), 25.5% PEG 4,000 and 15% v/v glycerol, vapor diffusion, sitting drop, temperature 282K

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Data collection

DiffractionMean temperature: 100 K
Diffraction sourceSource: SYNCHROTRON / Site: APS / Beamline: 21-ID-G / Wavelength: 0.97857 Å
DetectorType: RAYONIX MX-300 / Detector: CCD / Date: Feb 15, 2012
RadiationMonochromator: C(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.97857 Å / Relative weight: 1
ReflectionNumber: 1029210 / Rmerge(I) obs: 0.148 / D res high: 2.1 Å / Num. obs: 244679 / % possible obs: 99.5
Diffraction reflection shell
Highest resolution (Å)Lowest resolution (Å)Num. obs% possible obs (%)IDRmerge(I) obs
9.3930263895.810.037
6.649.39503499.710.045
5.426.64651399.810.058
4.75.42769199.710.052
4.24.7868099.910.052
3.834.2966199.710.057
3.553.831048399.810.069
3.323.551125999.810.088
3.133.321198199.410.109
2.973.131264299.410.143
2.832.971325199.510.173
2.712.831385898.910.207
2.62.711450199.410.244
2.512.61497299.210.274
2.422.511563599.510.321
2.352.421613399.410.36
2.282.351673199.510.405
2.212.281717399.610.471
2.152.211776899.910.525
2.12.151807599.810.609
ReflectionResolution: 2.1→30 Å / Num. obs: 118199 / % possible obs: 99.5 % / Observed criterion σ(F): 0 / Observed criterion σ(I): -3 / Biso Wilson estimate: 23.762 Å2 / Rsym value: 0.148 / Net I/σ(I): 9.24
Reflection shell
Resolution (Å)Rmerge(I) obsMean I/σ(I) obsNum. measured obsNum. unique obsDiffraction-ID% possible all
2.1-2.150.6092.447769118075199.8
2.15-2.210.5252.827628417768199.9
2.21-2.280.4713.197369817173199.6
2.28-2.350.4053.77181316731199.5
2.35-2.420.364.176948116133199.4
2.42-2.510.3214.696712415635199.5
2.51-2.60.2745.476403714972199.2
2.6-2.710.2446.176207014501199.4
2.71-2.830.2077.265892313858198.9
2.83-2.970.1738.65612713251199.5
2.97-3.130.14310.155303912642199.4
3.13-3.320.10912.734947711981199.4
3.32-3.550.08815.244626611259199.8
3.55-3.830.06918.094248010483199.8
3.83-4.20.05720.58387879661199.7
4.2-4.70.05221.87350348680199.9
4.7-5.420.05221.64308627691199.7
5.42-6.640.05820.33263556513199.8
6.64-9.390.04524.18198195034199.7
9.39-300.03727.3898432638195.8

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Phasing

PhasingMethod: molecular replacement

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Processing

Software
NameVersionClassificationNB
XSCALEdata scaling
PHASERphasing
PHENIX1.8_1066refinement
PDB_EXTRACT3.11data extraction
HKL-2000data collection
XDSdata reduction
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: PDB entry 1EC7

1ec7


Resolution: 2.1→29.965 Å / Occupancy max: 1 / Occupancy min: 0.4 / FOM work R set: 0.9051 / SU ML: 0.19 / Cross valid method: THROUGHOUT / σ(F): 1.99 / Phase error: 17.23 / Stereochemistry target values: ML
RfactorNum. reflection% reflectionSelection details
Rfree0.1863 6221 5 %random
Rwork0.1486 ---
all0.172 ---
obs0.1505 118199 99.91 %-
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso max: 75.71 Å2 / Biso mean: 16.293 Å2 / Biso min: 2.39 Å2
Refinement stepCycle: LAST / Resolution: 2.1→29.965 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms13568 0 102 1450 15120
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.00814035
X-RAY DIFFRACTIONf_angle_d1.02919050
X-RAY DIFFRACTIONf_chiral_restr0.0722065
X-RAY DIFFRACTIONf_plane_restr0.0052507
X-RAY DIFFRACTIONf_dihedral_angle_d13.0035122
LS refinement shell

Refine-ID: X-RAY DIFFRACTION / Total num. of bins used: 30 / % reflection obs: 100 %

Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkNum. reflection all
2.1-2.12390.24222070.182839394146
2.1239-2.14890.26142060.178439094115
2.1489-2.17510.22522070.173239304137
2.1751-2.20260.21582050.169739054110
2.2026-2.23150.2312080.169139504158
2.2315-2.26210.23552070.170439284135
2.2621-2.29440.25752080.173539424150
2.2944-2.32860.23852050.169938974102
2.3286-2.3650.22662060.164539194125
2.365-2.40380.2282080.158239604168
2.4038-2.44520.22592060.164239114117
2.4452-2.48970.20952060.159239064112
2.4897-2.53750.20042070.156539474154
2.5375-2.58930.20892090.152939534162
2.5893-2.64560.20912050.149639044109
2.6456-2.70710.20862050.155738954100
2.7071-2.77470.20542070.153339404147
2.7747-2.84970.18782080.149839474155
2.8497-2.93350.19082060.148739184124
2.9335-3.02810.20972080.153839414149
3.0281-3.13620.17732070.149739434150
3.1362-3.26160.19572070.142839274134
3.2616-3.40980.16352080.13639584166
3.4098-3.58930.15492080.133439554163
3.5893-3.81380.15082090.124539544163
3.8138-4.10760.15522070.120539494156
4.1076-4.51970.15122090.124539734182
4.5197-5.17080.12192100.130539714181
5.1708-6.50370.15792090.158739894198
6.5037-29.96780.16382130.157340434256
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
10.72750.41620.06790.2515-0.02330.6919-0.0752-0.0054-0.03890.0878-0.0562-0.3068-0.04010.07370.13460.03990.01280.0130.0691-0.01630.0855.285455.2871107.2595
20.48150.1731-0.27830.6579-0.29560.4262-0.0092-0.07260.07280.05550.0276-0.0006-0.05580.0179-0.00150.0736-0.0202-0.02560.1079-0.01010.065949.270551.2806113.6301
30.1970.03770.00020.7297-0.240.5344-0.03770.0070.0245-0.02030.0053-0.0249-0.03220.02480.03530.0511-0.0351-0.00750.08290.00340.081348.382353.9665100.6601
40.4950.00340.0920.4050.16920.28330.00380.18330.0985-0.10570.0359-0.2108-0.09310.0664-0.02670.1-0.0782-0.00030.18550.06360.262565.272367.554786.1751
51.3886-1.3345-0.35613.48160.61081.45760.03440.2080.1883-0.1895-0.0357-0.0201-0.0921-0.0654-0.0190.0665-0.07070.00440.14840.04440.104350.795967.788779.9947
62.5391-0.2262-0.45290.76360.16381.4960.01240.17940.2127-0.221-0.04330.01-0.15860.01720.01380.0799-0.0344-0.02590.0840.03650.091442.180367.954680.4971
70.3877-0.2507-0.03040.3664-0.11760.1473-0.0010.064-0.0167-0.01070.0130.03920.0009-0.0283-0.00850.0696-0.0228-0.00530.09510.01140.071338.600857.673591.4844
80.82140.6435-0.12731.31020.07780.7233-0.06730.0907-0.0019-0.09220.0522-0.13560.05420.08310.00480.0782-0.03190.00090.08320.0130.109858.282852.996892.5605
90.5854-0.2790.19880.6749-0.32870.4062-0.0646-0.0470.20770.123-0.0548-0.2118-0.12130.04640.10360.1256-0.0395-0.03730.12970.00650.174156.790666.3212102.3411
100.7540.04330.0750.34630.02330.23070.0653-0.0043-0.24380.01050.10030.00190.29910.0318-0.09740.1355-0.0661-0.01320.0630.03930.178329.383819.6461103.3568
112.01971.66120.35052.26810.56681.3746-0.0460.2061-0.0889-0.23960.0624-0.04480.17770.0508-0.01930.1532-0.00310.0190.0776-0.01320.095939.679123.995394.6452
120.91050.12620.33080.2670.00670.44580.0432-0.048-0.1251-0.050.04630.05920.1028-0.0821-0.06530.1014-0.0353-0.00470.07450.03180.112726.072429.5326101.9838
130.2711-0.0850.11970.36830.0790.3553-0.0144-0.3381-0.13280.0052-0.01850.36220.0188-0.4655-0.04210.1032-0.17230.04510.2060.22890.21063.156125.8794109.1806
141.1963-0.241-0.68850.8842-0.36791.64090.0032-0.04540.00670.09630.02480.2759-0.0925-0.04840.00240.0714-0.03590.00820.10390.01720.16192.879241.27898.9691
150.1929-0.13510.10160.2165-0.22010.3618-0.00760.00560.00050.0060.02940.0473-0.0038-0.0212-0.01910.0803-0.0379-0.00530.09650.01340.084419.443940.449997.2504
161.0280.484-0.13411.07780.28530.51440.009-0.1274-0.08320.10990.06290.13350.131-0.0591-0.08160.1169-0.0404-0.01080.14620.04840.098219.500327.5146112.8012
171.72080.9871.24352.9271.0442.18370.0998-0.1093-0.33860.30.0192-0.08820.39190.0162-0.12690.139-0.03740.00940.15120.0720.139928.835116.344110.2572
180.15330.0323-0.06261.0568-0.29810.6182-0.06460.117-0.1042-0.0871-0.0053-0.00680.1835-0.06710.06870.1559-0.0773-0.0350.14730.02980.21316.420920.213490.128
191.8009-1.3035-0.16881.00770.0630.11520.0630.138-0.0486-0.0495-0.08850.07630.007-0.0250.00230.0758-0.0052-0.03560.0733-0.01090.08110.203558.562553.6843
201.0456-0.2443-0.2493.58262.45251.986-0.0097-0.2168-0.2790.46090.01240.10250.3952-0.0484-0.0210.1113-0.0051-0.00920.07130.03320.1366-8.429459.689966.1568
210.591-0.4272-0.29930.5120.44290.47530.0134-0.00970.0135-0.0176-0.00830.02080.0544-0.0253-0.00820.066-0.0033-0.00570.06810.01310.05078.474665.127359.5421
220.67480.45680.41582.77390.07791.13140.01370.34110.0684-0.5268-0.0832-0.0402-0.0880.17610.04230.13840.04750.01490.1632-0.01010.049322.388753.207342.4071
230.8353-0.02290.08291.2861-0.37211.15410.08430.01660.00920.0224-0.0746-0.12740.03270.1703-0.00730.0620.01530.00740.0786-0.00110.066529.778351.241859.772
241.11690.7192-0.92391.4154-1.4342.8288-0.0093-0.0719-0.0852-0.0204-0.0159-0.13370.0890.15710.04370.07970.0067-0.00540.05290.00320.084427.405149.029172.8541
250.0685-0.05880.06330.1768-0.17180.29090.015-0.0324-0.036-0.0164-0.0324-0.03120.0366-0.02960.01650.06760.00310.00470.06640.00310.057514.75958.856271.7565
261.1172-0.5788-0.53791.1225-0.05170.40130.06690.0806-0.0167-0.133-0.06310.0372-0.0147-0.065-0.02130.0531-0.00450.00520.05570.00640.069313.464463.854256.1004
270.85170.0241-0.07611.84110.10870.5728-0.08750.21950.137-0.2165-0.02650.2573-0.1066-0.060.11440.08330.0295-0.01040.12670.01180.07344.974566.690348.2409
282.1525-0.38051.65271.3947-0.44672.9403-0.0769-0.0425-0.07670.14410.00440.12250.0915-0.160.06650.1557-0.02160.0050.0906-0.01810.174614.093738.01954.7051
290.34360.10250.07370.5732-0.40551.6047-0.0517-0.0340.03850.0079-0.03440.0267-0.1574-0.03040.07490.09520.00030.00920.048-0.01340.08743.848691.378586.427
303.9317-0.01124.3790.05850.00064.8679-0.00370.1928-0.03830.0581-0.0286-0.1769-0.04510.39080.04950.089-0.0281-0.00490.11590.00850.14614.583682.017878.9541
310.30230.0159-0.06510.2857-0.04651.0720.0197-0.02070.06920.0561-0.02630.0195-0.0373-0.02650.00860.06290.00970.00530.0474-0.00160.0660.103279.447188.0709
320.88490.08150.1450.6378-0.36630.4153-0.0352-0.19210.01860.24210.05670.1394-0.0732-0.1137-0.02720.1995-0.00260.07520.1779-0.03270.1159-8.119182.8949110.6919
331.3377-0.1698-0.05310.58010.07510.6106-0.0371-0.1854-0.11970.2410.04010.01830.1148-0.06930.00290.19710.01330.02470.15410.03790.06923.357567.8917109.0841
344.0558-0.4265-0.6930.7980.41360.84520.0233-0.3099-0.25960.1207-0.01510.0510.0514-0.07590.00790.0994-0.0063-0.00520.12720.05610.070514.454362.9954102.6477
350.3866-0.05160.04230.1742-0.03810.2196-0.0382-0.0248-0.02250.03590.012-0.01590.00940.03730.02330.07050.0042-0.00250.072-0.00410.05256.844770.807489.0722
361.2601-0.2681-0.03320.7921-0.14151.4152-0.0076-0.16460.01070.03090.05940.1447-0.0334-0.1144-0.02470.06570.00140.01410.08410.00050.0791-8.073179.149894.1722
370.387-0.01920.37610.5196-0.07890.8596-0.049-0.060.08160.11280.0074-0.0418-0.20920.05450.04810.1621-0.01140.00990.1269-0.03030.13063.951389.837999.6469
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection detailsAuth asym-IDAuth seq-ID
1X-RAY DIFFRACTION1CHAIN A AND (RESID 3:34 )A0
2X-RAY DIFFRACTION2CHAIN A AND (RESID 35:90 )A0
3X-RAY DIFFRACTION3CHAIN A AND (RESID 91:159 )A0
4X-RAY DIFFRACTION4CHAIN A AND (RESID 160:199 )A0
5X-RAY DIFFRACTION5CHAIN A AND (RESID 200:226 )A0
6X-RAY DIFFRACTION6CHAIN A AND (RESID 227:251 )A0
7X-RAY DIFFRACTION7CHAIN A AND (RESID 252:357 )A0
8X-RAY DIFFRACTION8CHAIN A AND (RESID 358:391 )A0
9X-RAY DIFFRACTION9CHAIN A AND (RESID 392:446 )A0
10X-RAY DIFFRACTION10CHAIN B AND (RESID 4:58 )B0
11X-RAY DIFFRACTION11CHAIN B AND (RESID 59:90 )B0
12X-RAY DIFFRACTION12CHAIN B AND (RESID 91:159 )B0
13X-RAY DIFFRACTION13CHAIN B AND (RESID 160:226 )B0
14X-RAY DIFFRACTION14CHAIN B AND (RESID 227:254 )B0
15X-RAY DIFFRACTION15CHAIN B AND (RESID 255:357 )B0
16X-RAY DIFFRACTION16CHAIN B AND (RESID 358:391 )B0
17X-RAY DIFFRACTION17CHAIN B AND (RESID 392:416 )B0
18X-RAY DIFFRACTION18CHAIN B AND (RESID 417:446 )B0
19X-RAY DIFFRACTION19CHAIN C AND (RESID 6:57 )C0
20X-RAY DIFFRACTION20CHAIN C AND (RESID 58:106 )C0
21X-RAY DIFFRACTION21CHAIN C AND (RESID 107:158 )C0
22X-RAY DIFFRACTION22CHAIN C AND (RESID 159:198 )C0
23X-RAY DIFFRACTION23CHAIN C AND (RESID 199:247 )C0
24X-RAY DIFFRACTION24CHAIN C AND (RESID 248:281 )C0
25X-RAY DIFFRACTION25CHAIN C AND (RESID 282:343 )C0
26X-RAY DIFFRACTION26CHAIN C AND (RESID 344:376 )C0
27X-RAY DIFFRACTION27CHAIN C AND (RESID 377:414 )C0
28X-RAY DIFFRACTION28CHAIN C AND (RESID 415:446 )C0
29X-RAY DIFFRACTION29CHAIN D AND (RESID 6:75 )D0
30X-RAY DIFFRACTION30CHAIN D AND (RESID 76:100 )D0
31X-RAY DIFFRACTION31CHAIN D AND (RESID 101:158 )D0
32X-RAY DIFFRACTION32CHAIN D AND (RESID 159:198 )D0
33X-RAY DIFFRACTION33CHAIN D AND (RESID 199:247 )D0
34X-RAY DIFFRACTION34CHAIN D AND (RESID 248:281 )D0
35X-RAY DIFFRACTION35CHAIN D AND (RESID 282:356 )D0
36X-RAY DIFFRACTION36CHAIN D AND (RESID 357:390 )D0
37X-RAY DIFFRACTION37CHAIN D AND (RESID 391:445 )D0

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