- PDB-4g8t: Crystal structure of a glucarate dehydratase related protein, fro... -
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Basic information
Entry
Database: PDB / ID: 4g8t
Title
Crystal structure of a glucarate dehydratase related protein, from actinobacillus succinogenes, target EFI-502312, with sodium and sulfate bound, ordered loop
Mass: 18.015 Da / Num. of mol.: 1973 / Source method: isolated from a natural source / Formula: H2O
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 2.34 Å3/Da / Density % sol: 47.39 %
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Temperature: 298 K / Method: sitting drop vapor diffuction / pH: 7.5 Details: Protein (10 mM Hepes pH 7.5, 150 mM NaCl, 10% glycerol, 1 mM DTT, 5 mM MgCl); Reservoir (0.2 M Lithium Sulfate, 0.1 M Sodium Acetate, 0.1 M HEPES, 25% PEG4000); Cryoprotection (Reservoir, + ...Details: Protein (10 mM Hepes pH 7.5, 150 mM NaCl, 10% glycerol, 1 mM DTT, 5 mM MgCl); Reservoir (0.2 M Lithium Sulfate, 0.1 M Sodium Acetate, 0.1 M HEPES, 25% PEG4000); Cryoprotection (Reservoir, + 20% glycerol and 50 mM MgCl), sitting drop vapor diffuction, temperature 298K