- PDB-4ggh: Crystal structure of an enolase family member from vibrio harveyi... -
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Basic information
Entry
Database: PDB / ID: 4ggh
Title
Crystal structure of an enolase family member from vibrio harveyi (efi-target 501692) with homology to mannonate dehydratase, with mg, hepes, and ethylene glycol bound (ordered loops, space group c2221)
A: Putative uncharacterized protein B: Putative uncharacterized protein C: Putative uncharacterized protein D: Putative uncharacterized protein hetero molecules
A: Putative uncharacterized protein B: Putative uncharacterized protein C: Putative uncharacterized protein D: Putative uncharacterized protein hetero molecules
A: Putative uncharacterized protein B: Putative uncharacterized protein C: Putative uncharacterized protein D: Putative uncharacterized protein hetero molecules
Mass: 18.015 Da / Num. of mol.: 1694 / Source method: isolated from a natural source / Formula: H2O
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 2.63 Å3/Da / Density % sol: 53.25 %
Crystal grow
Temperature: 298 K / Method: vapor diffusion, sitting drop / pH: 7.5 Details: Protein (10 mM Hepes, 150 mM NaCl, 10% glycerol, 1 mM DTT, 5 mM MgCl); Reservoir (200 mM NaCl, 0.1 M Hepes, 25% Peg3350); Cryoprotection (Reservoir, + 20% ethylene glycol and 50 mM MgCl), pH ...Details: Protein (10 mM Hepes, 150 mM NaCl, 10% glycerol, 1 mM DTT, 5 mM MgCl); Reservoir (200 mM NaCl, 0.1 M Hepes, 25% Peg3350); Cryoprotection (Reservoir, + 20% ethylene glycol and 50 mM MgCl), pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K