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- PDB-4ej3: Crystal structure of a CRISPR associated protein from Thermus the... -
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Open data
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Basic information
Entry | Database: PDB / ID: 4ej3 | ||||||
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Title | Crystal structure of a CRISPR associated protein from Thermus thermophilus HB8 | ||||||
![]() | CRISPR associated protein | ||||||
![]() | SIGNALING PROTEIN / CRISPR / cascade protein | ||||||
Function / homology | Topoisomerase I; Chain A, domain 4 - #100 / CRISPR-associated protein Cse1 / CRISPR-associated protein Cse1 (CRISPR_cse1) / Topoisomerase I; Chain A, domain 4 / defense response to virus / Orthogonal Bundle / Mainly Alpha / CRISPR-associated protein CasA/Cse1![]() | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Huang, Q.Q. | ||||||
![]() | ![]() Title: Crystal structure of a CRISPR associated protein from Thermus thermophilus HB8 Authors: Huang, Q.Q. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 192.5 KB | Display | ![]() |
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PDB format | ![]() | 153 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 434.2 KB | Display | ![]() |
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Full document | ![]() | 442.3 KB | Display | |
Data in XML | ![]() | 33.7 KB | Display | |
Data in CIF | ![]() | 47.6 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 57678.680 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.49 Å3/Da / Density % sol: 50.62 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 6.5 Details: 2.5M sodium formate, 0.1M Mes, 5% DMSO, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
-Data collection
Diffraction |
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Diffraction source |
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Detector |
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Radiation |
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Radiation wavelength |
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Reflection | Resolution: 2.5→50 Å / Num. all: 38600 / Num. obs: 38561 / % possible obs: 99.9 % / Observed criterion σ(F): 1 / Observed criterion σ(I): 1 | ||||||||||||||||||
Reflection shell | Resolution: 2.5→2.54 Å / Redundancy: 3.7 % / Mean I/σ(I) obs: 36.2 / Num. unique all: 1896 / Rsym value: 0.331 / % possible all: 100 |
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Processing
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Refinement | Method to determine structure: ![]()
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.4 Å / Solvent model: MASK | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 36.765 Å2
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Refinement step | Cycle: LAST / Resolution: 2.52→49.86 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.52→2.588 Å / Total num. of bins used: 20
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