PROTEIN FIBRIL / amyloid / out-of-register / fiber-forming / macrocycle
機能・相同性
機能・相同性情報
plus-end-directed organelle transport along microtubule / histone-dependent DNA binding / negative regulation of establishment of protein localization to mitochondrion / neurofibrillary tangle / microtubule lateral binding / axonal transport / main axon / phosphatidylinositol bisphosphate binding / regulation of long-term synaptic depression / tubulin complex ...plus-end-directed organelle transport along microtubule / histone-dependent DNA binding / negative regulation of establishment of protein localization to mitochondrion / neurofibrillary tangle / microtubule lateral binding / axonal transport / main axon / phosphatidylinositol bisphosphate binding / regulation of long-term synaptic depression / tubulin complex / positive regulation of protein localization to synapse / negative regulation of tubulin deacetylation / generation of neurons / regulation of chromosome organization / rRNA metabolic process / axonal transport of mitochondrion / regulation of mitochondrial fission / axon development / central nervous system neuron development / intracellular distribution of mitochondria / regulation of microtubule polymerization / microtubule polymerization / lipoprotein particle binding / minor groove of adenine-thymine-rich DNA binding / dynactin binding / negative regulation of mitochondrial membrane potential / apolipoprotein binding / axolemma / protein polymerization / glial cell projection / negative regulation of mitochondrial fission / Caspase-mediated cleavage of cytoskeletal proteins / regulation of microtubule polymerization or depolymerization / positive regulation of axon extension / neurofibrillary tangle assembly / Activation of AMPK downstream of NMDARs / regulation of cellular response to heat / synapse assembly / supramolecular fiber organization / positive regulation of protein localization / regulation of calcium-mediated signaling / somatodendritic compartment / cellular response to brain-derived neurotrophic factor stimulus / cytoplasmic microtubule organization / positive regulation of microtubule polymerization / axon cytoplasm / stress granule assembly / phosphatidylinositol binding / regulation of microtubule cytoskeleton organization / nuclear periphery / protein phosphatase 2A binding / positive regulation of superoxide anion generation / cellular response to reactive oxygen species / astrocyte activation / Hsp90 protein binding / microglial cell activation / cellular response to nerve growth factor stimulus / response to lead ion / synapse organization / PKR-mediated signaling / protein homooligomerization / regulation of synaptic plasticity / SH3 domain binding / memory / microtubule cytoskeleton organization / cytoplasmic ribonucleoprotein granule / neuron projection development / cell-cell signaling / single-stranded DNA binding / protein-folding chaperone binding / actin binding / cellular response to heat / microtubule cytoskeleton / cell body / growth cone / double-stranded DNA binding / microtubule binding / protein-macromolecule adaptor activity / dendritic spine / sequence-specific DNA binding / amyloid fibril formation / microtubule / learning or memory / neuron projection / regulation of autophagy / membrane raft / axon / negative regulation of gene expression / neuronal cell body / dendrite / DNA damage response / protein kinase binding / enzyme binding / mitochondrion / DNA binding / RNA binding / extracellular region / identical protein binding / nucleus / plasma membrane 類似検索 - 分子機能
Microtubule-associated protein Tau / Microtubule associated protein, tubulin-binding repeat / Tau and MAP protein, tubulin-binding repeat / Tau and MAP proteins tubulin-binding repeat signature. / Tau and MAP proteins tubulin-binding repeat profile. / : 類似検索 - ドメイン・相同性
PHOSPHATE ION / Microtubule-associated protein tau 類似検索 - 構成要素
プロトコル: SINGLE WAVELENGTH / 単色(M)・ラウエ(L): M / 散乱光タイプ: x-ray
放射波長
波長: 0.91609 Å / 相対比: 1
反射
解像度: 1.75→18.22 Å / % possible obs: 99 % / Observed criterion σ(I): -3 / Biso Wilson estimate: 15.82 Å2 / Rmerge(I) obs: 0.086 / Net I/σ(I): 7.83
反射 シェル
解像度 (Å)
最高解像度 (Å)
Rmerge(I) obs
Mean I/σ(I) obs
Diffraction-ID
% possible all
1.75-1.8
0.424
2.5
1
98.7
1.8-1.84
0.414
2.55
1
98.8
1.84-1.9
0.286
3.52
1
99.7
1.9-1.96
0.225
4.23
1
99.3
1.96-2.02
0.162
4.99
1
99.5
2.02-2.09
0.138
6.15
1
99.4
2.09-2.17
0.125
7.11
1
99.5
2.17-2.26
0.132
7.12
1
99.3
2.26-2.36
0.118
7.84
1
100
2.36-2.48
0.108
8.02
1
98.4
2.48-2.61
0.109
8.8
1
99.8
2.61-2.77
0.083
10.4
1
99.3
2.77-2.96
0.085
10.77
1
99.5
2.96-3.2
0.068
12.43
1
98.4
3.2-3.5
0.058
14.11
1
100
3.5-3.91
0.058
15.04
1
98.6
3.91-4.52
0.056
15.53
1
97.3
4.52-5.53
0.041
16.41
1
97.2
5.53-7.83
0.052
15.66
1
95
7.83
0.044
14.97
1
89.4
-
解析
ソフトウェア
名称
バージョン
分類
NB
XSCALE
データスケーリング
REFMAC
精密化
PDB_EXTRACT
3.1
データ抽出
BUSTER
2.10.0
精密化
精密化
構造決定の手法: 単波長異常分散 / 解像度: 1.75→18.22 Å / Cor.coef. Fo:Fc: 0.9309 / Cor.coef. Fo:Fc free: 0.9272 / Occupancy max: 1 / Occupancy min: 0.5 / SU B: 4.937 / SU ML: 0.075 / SU R Cruickshank DPI: 0.1287 / 交差検証法: THROUGHOUT / σ(F): 0 / ESU R: 0.129 / ESU R Free: 0.109 / 立体化学のターゲット値: MAXIMUM LIKELIHOOD 詳細: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS; U VALUES: RESIDUAL ONLY
Rfactor
反射数
%反射
Selection details
Rfree
0.2213
642
9.85 %
RANDOM
Rwork
0.1854
-
-
-
obs
0.1889
6519
99.86 %
-
溶媒の処理
イオンプローブ半径: 0.8 Å / 減衰半径: 0.8 Å / VDWプローブ半径: 1.4 Å / 溶媒モデル: MASK