+Open data
-Basic information
Entry | Database: PDB / ID: 4dep | ||||||
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Title | Structure of the IL-1b signaling complex | ||||||
Components |
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Keywords | IMMUNE SYSTEM / b-trefoil / immunoglobulin / extracellular | ||||||
Function / homology | Function and homology information positive regulation of interleukin-1-mediated signaling pathway / interleukin-1, type I, activating receptor activity / Interleukin-33 signaling / interleukin-33 receptor activity / positive regulation of neutrophil extravasation / Interleukin-36 pathway / interleukin-1 receptor activity / trans-synaptic signaling by trans-synaptic complex / interleukin-1 binding / Receptor-type tyrosine-protein phosphatases ...positive regulation of interleukin-1-mediated signaling pathway / interleukin-1, type I, activating receptor activity / Interleukin-33 signaling / interleukin-33 receptor activity / positive regulation of neutrophil extravasation / Interleukin-36 pathway / interleukin-1 receptor activity / trans-synaptic signaling by trans-synaptic complex / interleukin-1 binding / Receptor-type tyrosine-protein phosphatases / smooth muscle adaptation / positive regulation of T cell mediated immunity / positive regulation of myosin light chain kinase activity / negative regulation of adiponectin secretion / monocyte aggregation / negative regulation of lipid metabolic process / positive regulation of lipid catabolic process / negative regulation of glucose transmembrane transport / regulation of nitric-oxide synthase activity / regulation of postsynaptic density assembly / hyaluronan biosynthetic process / positive regulation of cell adhesion molecule production / positive regulation of T-helper 1 cell cytokine production / synaptic membrane adhesion / positive regulation of complement activation / positive regulation of calcidiol 1-monooxygenase activity / cellular response to interleukin-17 / sequestering of triglyceride / positive regulation of RNA biosynthetic process / negative regulation of gap junction assembly / interleukin-33-mediated signaling pathway / positive regulation of prostaglandin secretion / positive regulation of prostaglandin biosynthetic process / positive regulation of immature T cell proliferation in thymus / vascular endothelial growth factor production / positive regulation of interleukin-13 production / positive regulation of neuroinflammatory response / positive regulation of interleukin-5 production / ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase / regulation of defense response to virus by host / fever generation / NAD+ nucleosidase activity / positive regulation of fever generation / CLEC7A/inflammasome pathway / regulation of establishment of endothelial barrier / neutrophil activation / NAD+ nucleotidase, cyclic ADP-ribose generating / platelet-derived growth factor receptor binding / Interleukin-1 processing / response to carbohydrate / interleukin-1 receptor binding / positive regulation of monocyte chemotactic protein-1 production / positive regulation of vascular endothelial growth factor receptor signaling pathway / positive regulation of heterotypic cell-cell adhesion / negative regulation of synaptic transmission / positive regulation of synapse assembly / positive regulation of membrane protein ectodomain proteolysis / regulation of canonical NF-kappaB signal transduction / positive regulation of granulocyte macrophage colony-stimulating factor production / interleukin-1-mediated signaling pathway / positive regulation of p38MAPK cascade / response to ATP / : / positive regulation of interleukin-4 production / macrophage chemotaxis / Interleukin-10 signaling / regulation of insulin secretion / positive regulation of cell division / regulation of presynapse assembly / regulation of neurogenesis / negative regulation of extrinsic apoptotic signaling pathway in absence of ligand / positive regulation of vascular endothelial growth factor production / ectopic germ cell programmed cell death / Pyroptosis / negative regulation of lipid catabolic process / coreceptor activity / Purinergic signaling in leishmaniasis infection / positive regulation of epithelial to mesenchymal transition / positive regulation of T cell proliferation / positive regulation of glial cell proliferation / JNK cascade / extrinsic apoptotic signaling pathway in absence of ligand / positive regulation of interleukin-2 production / negative regulation of insulin receptor signaling pathway / embryo implantation / response to interleukin-1 / positive regulation of mitotic nuclear division / neutrophil chemotaxis / regulation of ERK1 and ERK2 cascade / positive regulation of protein export from nucleus / negative regulation of MAP kinase activity / secretory granule / cytokine activity / positive regulation of interleukin-8 production / astrocyte activation / positive regulation of JNK cascade / positive regulation of MAP kinase activity / cytokine-mediated signaling pathway / positive regulation of DNA-binding transcription factor activity / negative regulation of neurogenesis Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 3.1 Å | ||||||
Authors | Thomas, C. / Garcia, K.C. | ||||||
Citation | Journal: Nat.Struct.Mol.Biol. / Year: 2012 Title: Structure of the activating IL-1 receptor signaling complex. Authors: Thomas, C. / Bazan, J.F. / Garcia, K.C. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 4dep.cif.gz | 298.6 KB | Display | PDBx/mmCIF format |
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PDB format | pdb4dep.ent.gz | 242.5 KB | Display | PDB format |
PDBx/mmJSON format | 4dep.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 4dep_validation.pdf.gz | 505.2 KB | Display | wwPDB validaton report |
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Full document | 4dep_full_validation.pdf.gz | 543.8 KB | Display | |
Data in XML | 4dep_validation.xml.gz | 55.2 KB | Display | |
Data in CIF | 4dep_validation.cif.gz | 74.6 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/de/4dep ftp://data.pdbj.org/pub/pdb/validation_reports/de/4dep | HTTPS FTP |
-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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-Components
#1: Protein | Mass: 17807.289 Da / Num. of mol.: 2 / Fragment: UNP residues 117-269 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: IL-1b, IL1B, IL1F2 / Plasmid: pGEX-6P-1 / Production host: Escherichia coli (E. coli) / References: UniProt: P01584 #2: Protein | Mass: 36967.965 Da / Num. of mol.: 2 / Fragment: UNP residues 18-336 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: IL-1RI, IL1R, IL1R1, IL1RA, IL1RT1 / Production host: Homo sapiens (human) / References: UniProt: P14778 #3: Protein | Mass: 40107.699 Da / Num. of mol.: 2 / Fragment: UNP residues 21-367 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: C3orf13, IL-1RAcP, IL1R3, IL1RAP / Production host: Homo sapiens (human) / References: UniProt: Q9NPH3 #4: Sugar | ChemComp-NAG / #5: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 3.01 Å3/Da / Density % sol: 59.1 % |
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Crystal grow | Temperature: 293 K / pH: 6.5 Details: 12% PEG 5000 MME, 100 mM MES pH 6.5, 12% 1-propanol, protein at 12 mg/mL, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: ALS / Beamline: 8.2.1 / Wavelength: 1 |
Detector | Type: ADSC Q315R / Detector: CCD / Date: Sep 8, 2011 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 3.1→19.7 Å / Num. obs: 41079 / % possible obs: 96.3 % / Redundancy: 1.7 % / Rmerge(I) obs: 0.068 / Net I/σ(I): 10.5 |
Reflection shell | Resolution: 3.1→3.2 Å / Redundancy: 1.7 % / Rmerge(I) obs: 0.361 / Mean I/σ(I) obs: 2.2 / % possible all: 98.7 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT / Resolution: 3.1→19.7 Å / SU ML: 0.95 / σ(F): 1.99 / Phase error: 26.07 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL / Bsol: 21.56 Å2 / ksol: 0.3 e/Å3 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 3.1→19.7 Å
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Refine LS restraints |
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LS refinement shell |
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