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Yorodumi- PDB-4cqi: Crystal structure of recombinant tubulin-binding cofactor A (TBCA... -
+Open data
-Basic information
Entry | Database: PDB / ID: 4cqi | ||||||
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Title | Crystal structure of recombinant tubulin-binding cofactor A (TBCA) from Leishmania major | ||||||
Components | TUBULIN-BINDING COFACTOR A | ||||||
Keywords | STRUCTURAL PROTEIN / TUBULIN-BINDING COFACTOR A | ||||||
Function / homology | Function and homology information post-chaperonin tubulin folding pathway / tubulin complex assembly / beta-tubulin binding / tubulin binding / microtubule cytoskeleton / protein folding / microtubule / cytosol Similarity search - Function | ||||||
Biological species | LEISHMANIA MAJOR (eukaryote) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / OTHER / Resolution: 1.9 Å | ||||||
Authors | Barrack, K.L. / Fyfe, P.K. / Hunter, W.N. | ||||||
Citation | Journal: Acta Crystallogr.,Sect.F / Year: 2015 Title: The Structure of Tubulin-Binding Cofactor a from Leishmania Major Infers a Mode of Association During the Early Stages of Microtubule Assembly Authors: Barrack, K.L. / Fyfe, P.K. / Hunter, W.N. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 4cqi.cif.gz | 32.2 KB | Display | PDBx/mmCIF format |
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PDB format | pdb4cqi.ent.gz | 24.9 KB | Display | PDB format |
PDBx/mmJSON format | 4cqi.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/cq/4cqi ftp://data.pdbj.org/pub/pdb/validation_reports/cq/4cqi | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 14224.944 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) LEISHMANIA MAJOR (eukaryote) / Strain: FRIEDLIN / Plasmid: PET15B / Production host: ESCHERICHIA COLI (E. coli) / Strain (production host): BL21(DE3) / References: UniProt: Q9U1D9 |
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#2: Chemical | ChemComp-SO4 / |
#3: Chemical | ChemComp-GOL / |
#4: Water | ChemComp-HOH / |
Sequence details | TWO RESIDUES AT N-TERMINUS (GH) REMAIN FROM CLEAVED EXPRESSION |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.36 Å3/Da / Density % sol: 47.82 % / Description: NONE |
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Crystal grow | Details: RESERVOIR CONTAINED 0.2M (NH4)2HPO4 AND 1.6M (NH4)2SO4. |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: ESRF / Beamline: ID23-2 / Wavelength: 0.8726 |
Detector | Type: MARMOSAIC 225 mm CCD / Detector: CCD / Date: Feb 17, 2011 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.8726 Å / Relative weight: 1 |
Reflection | Resolution: 1.9→39.4 Å / Num. obs: 10821 / % possible obs: 100 % / Redundancy: 12.1 % / Rmerge(I) obs: 0.07 / Net I/σ(I): 24.9 |
Reflection shell | Resolution: 1.9→2 Å / Redundancy: 12.4 % / Rmerge(I) obs: 0.45 / Mean I/σ(I) obs: 6.8 / % possible all: 100 |
-Processing
Software |
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Refinement | Method to determine structure: OTHER Starting model: NONE Resolution: 1.9→39.43 Å / Cor.coef. Fo:Fc: 0.951 / Cor.coef. Fo:Fc free: 0.931 / SU B: 2.975 / SU ML: 0.09 / Cross valid method: THROUGHOUT / ESU R: 0.138 / ESU R Free: 0.134 / Stereochemistry target values: MAXIMUM LIKELIHOOD Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS. RESIDUES WITH INSUFFICIENT ELECTRON DENSITY WERE NOT MODELED.
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 23.981 Å2
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Refinement step | Cycle: LAST / Resolution: 1.9→39.43 Å
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Refine LS restraints |
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