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- PDB-4c76: Crystal Structure of the FMN-reductase Msue from Pseudomonas puti... -
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Open data
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Basic information
Entry | Database: PDB / ID: 4c76 | ||||||
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Title | Crystal Structure of the FMN-reductase Msue from Pseudomonas putida KT2440. | ||||||
![]() | FMN REDUCTASE (NADPH) | ||||||
![]() | OXIDOREDUCTASE / FLAVODOXIN-LIKE | ||||||
Function / homology | ![]() | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Gibson, R.P. / Isupov, M. / Littlechild, J.A. | ||||||
![]() | ![]() Title: Crystal Structure of the Fmn-Reductase Msue from Pseudomonas Putida Kt2440. Authors: Gibson, R.P. / Isupov, M. / Littlechild, J.A. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 92.1 KB | Display | ![]() |
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PDB format | ![]() | 75.5 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 453 KB | Display | ![]() |
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Full document | ![]() | 458 KB | Display | |
Data in XML | ![]() | 19.5 KB | Display | |
Data in CIF | ![]() | 27.9 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Components on special symmetry positions |
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Noncrystallographic symmetry (NCS) | NCS oper: (Code: given Matrix: (-0.9957, -0.09276, -0.002691), Vector: |
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Components
#1: Protein | Mass: 22615.367 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Description: DSMZ - GERMAN COLLECTION OF MICROORGANISMS (DSM-6125) Production host: ![]() ![]() #2: Chemical | #3: Chemical | ChemComp-PEG / #4: Chemical | #5: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.57 Å3/Da / Density % sol: 52.2 % / Description: NONE |
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Crystal grow | pH: 8.5 / Details: 0.1M TRIS-HCL PH 8.5, 25% W/V PEG 1K |
-Data collection
Diffraction | Mean temperature: 120 K | ||||||||||||
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Diffraction source | Source: ![]() ![]() ![]() | ||||||||||||
Detector | Type: MARRESEARCH / Detector: CCD | ||||||||||||
Radiation | Protocol: MAD / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | ||||||||||||
Radiation wavelength |
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Reflection | Resolution: 1.96→19.5 Å / Num. obs: 29204 / % possible obs: 99.9 % / Observed criterion σ(I): 2.5 / Redundancy: 9.4 % / Rmerge(I) obs: 0.12 / Net I/σ(I): 15.5 | ||||||||||||
Reflection shell | Resolution: 1.96→2.01 Å / Redundancy: 9.4 % / Rmerge(I) obs: 0.62 / Mean I/σ(I) obs: 3.5 / % possible all: 99.9 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: NONE Resolution: 1.96→19.5 Å / Cor.coef. Fo:Fc: 0.966 / Cor.coef. Fo:Fc free: 0.945 / SU B: 3.083 / SU ML: 0.088 / Cross valid method: THROUGHOUT / ESU R: 0.141 / ESU R Free: 0.134 / Stereochemistry target values: MAXIMUM LIKELIHOOD Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS. U VALUES REFINED INDIVIDUALLY
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 24.564 Å2
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Refinement step | Cycle: LAST / Resolution: 1.96→19.5 Å
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Refine LS restraints |
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