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- PDB-4c5m: Structure of the pyridoxal kinase from Staphylococcus aureus in c... -
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Open data
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Basic information
Entry | Database: PDB / ID: 4c5m | ||||||
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Title | Structure of the pyridoxal kinase from Staphylococcus aureus in complex with AMP-PCP | ||||||
![]() | PHOSPHOMETHYLPYRIMIDINE KINASE | ||||||
![]() | TRANSFERASE / RIBOKINASE | ||||||
Function / homology | ![]() phosphomethylpyrimidine kinase activity / hydroxymethylpyrimidine kinase activity / pyridoxal kinase / thiamine biosynthetic process / phosphorylation / nucleotide binding / cytosol Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Nodwell, M. / Alte, F. / Sieber, S.A. / Schneider, S. | ||||||
![]() | ![]() Title: A Subfamily of Bacterial Ribokinases Utilizes a Hemithioacetal for Pyridoxal Phosphate Salvage. Authors: Nodwell, M.B. / Koch, M.F. / Alte, F. / Schneider, S. / Sieber, S.A. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 480.8 KB | Display | ![]() |
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PDB format | ![]() | 397.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 3.5 MB | Display | ![]() |
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Full document | ![]() | 3.5 MB | Display | |
Data in XML | ![]() | 53.1 KB | Display | |
Data in CIF | ![]() | 76.5 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 4c5jSC ![]() 4c5kC ![]() 4c5lC ![]() 4c5nC S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS oper:
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Components
#1: Protein | Mass: 29811.793 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Production host: ![]() ![]() References: UniProt: Q99W31, UniProt: A0A0H3JTP0*PLUS, pyridoxal kinase #2: Chemical | ChemComp-ACP / #3: Chemical | ChemComp-SO4 / #4: Water | ChemComp-HOH / | Sequence details | FIRST AMINOACID IS A GLY INSTEAD OF MET DUE TO REMOVAL OF THE AFFINITY TAG | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.19 Å3/Da / Density % sol: 45 % / Description: NONE |
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Crystal grow | Details: 50MM HEPES, 2M NH4SO4 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS / Detector: PIXEL / Date: Jul 18, 2013 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 1.45→48.76 Å / Num. obs: 185724 / % possible obs: 99.5 % / Observed criterion σ(I): 1.5 / Redundancy: 8.8 % / Rmerge(I) obs: 0.06 / Net I/σ(I): 20.38 |
Reflection shell | Resolution: 1.45→1.5 Å / Redundancy: 8.5 % / Rmerge(I) obs: 1.17 / Mean I/σ(I) obs: 1.79 / % possible all: 96.3 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 4C5J Resolution: 1.45→48.76 Å / Cor.coef. Fo:Fc: 0.982 / Cor.coef. Fo:Fc free: 0.972 / SU B: 2.323 / SU ML: 0.039 / Cross valid method: THROUGHOUT / ESU R: 0.06 / ESU R Free: 0.059 / Stereochemistry target values: MAXIMUM LIKELIHOOD Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS. U VALUES REFINED INDIVIDUALLY
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 21.882 Å2
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Refinement step | Cycle: LAST / Resolution: 1.45→48.76 Å
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Refine LS restraints |
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