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Open data
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Basic information
Entry | Database: PDB / ID: 4c2k | ||||||
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Title | Crystal structure of human mitochondrial 3-ketoacyl-CoA thiolase | ||||||
![]() | 3-KETOACYL-COA THIOLASE, MITOCHONDRIAL | ||||||
![]() | TRANSFERASE / FATTY ACID METABOLISM / MITOCHONDRIAL BETA-OXIDATION / THIOLYTIC CLEAVAGE | ||||||
Function / homology | ![]() negative regulation of mitochondrial membrane permeability involved in apoptotic process / acetyl-CoA hydrolase / : / : / acetyl-CoA hydrolase activity / palmitoyl-CoA hydrolase / acetyl-CoA C-acyltransferase / acetyl-CoA C-acyltransferase activity / acetyl-CoA C-acetyltransferase / acetyl-CoA C-acetyltransferase activity ...negative regulation of mitochondrial membrane permeability involved in apoptotic process / acetyl-CoA hydrolase / : / : / acetyl-CoA hydrolase activity / palmitoyl-CoA hydrolase / acetyl-CoA C-acyltransferase / acetyl-CoA C-acyltransferase activity / acetyl-CoA C-acetyltransferase / acetyl-CoA C-acetyltransferase activity / fatty acyl-CoA hydrolase activity / Mitochondrial Fatty Acid Beta-Oxidation / Hydrolases; Acting on ester bonds; Thioester hydrolases / negative regulation of mitochondrial outer membrane permeabilization involved in apoptotic signaling pathway / fatty acid beta-oxidation / cholesterol biosynthetic process / cellular response to hypoxia / mitochondrial matrix / mitochondrion / RNA binding Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Kiema, T.-R. / Harijan, R.K. / Wierenga, R.K. | ||||||
![]() | ![]() Title: The Crystal Structure of Human Mitochondrial 3-Ketoacyl-Coa Thiolase (T1): Insight Into the Reaction Mechanism of its Thiolase and Thioesterase Activities Authors: Kiema, T.-R. / Harijan, R.K. / Strozyk, M. / Fukao, T. / Alexson, S.E.H. / Wierenga, R.K. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 308.9 KB | Display | ![]() |
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PDB format | ![]() | 249.7 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 492 KB | Display | ![]() |
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Full document | ![]() | 497.4 KB | Display | |
Data in XML | ![]() | 57.4 KB | Display | |
Data in CIF | ![]() | 81.6 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 4c2jC ![]() 1dm3S C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS oper:
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Components
#1: Protein | Mass: 44145.418 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #2: Chemical | ChemComp-EDO / #3: Chemical | ChemComp-DTT / | #4: Chemical | ChemComp-MES / #5: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.36 Å3/Da / Density % sol: 48 % / Description: NONE |
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Crystal grow | pH: 6.6 / Details: 100 MM MES PH 6.6, 15% MME-PEG5000 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC CCD / Detector: CCD / Date: Jun 17, 2011 / Details: TORROIDAL MIRROR |
Radiation | Monochromator: SI(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9393 Å / Relative weight: 1 |
Reflection | Resolution: 2→48.17 Å / Num. obs: 109515 / % possible obs: 99.6 % / Observed criterion σ(I): 3 / Redundancy: 3.7 % / Rmerge(I) obs: 0.07 / Net I/σ(I): 15.07 |
Reflection shell | Resolution: 2→2.1 Å / Redundancy: 3.7 % / Rmerge(I) obs: 0.41 / Mean I/σ(I) obs: 3.07 / % possible all: 99.8 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 1DM3 Resolution: 2→48.17 Å / Cor.coef. Fo:Fc: 0.924 / Cor.coef. Fo:Fc free: 0.902 / SU B: 3.525 / SU ML: 0.101 / Cross valid method: THROUGHOUT / ESU R: 0.189 / ESU R Free: 0.161 / Stereochemistry target values: MAXIMUM LIKELIHOOD Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS. U VALUES REFINED INDIVIDUALLY. SOME OR THE SIDE CHAIN ATOMS OF RESIDUES LYS A 38, LYS A 137, LYS A 143, GLU A 177, LYS A 209, LYS A 211, ...Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS. U VALUES REFINED INDIVIDUALLY. SOME OR THE SIDE CHAIN ATOMS OF RESIDUES LYS A 38, LYS A 137, LYS A 143, GLU A 177, LYS A 209, LYS A 211, LYS A 212, LYS A 214, GLN A 226, THR A 228, GLU A 230, LYS A 234, LYS A 241, LYS A 305, LYS A 312, MET B 1, LEU B 4, LYS B 137, LYS B 171, LYS B 173, GLU B 177, GLU B 178, LYS B 209, THR B 210, LYS B 214, GLN B 226, GLN B 233, LYS B 234, LYS B 269, LYS B 270, LYS B 305, LYS B 312, ILE B 338, LYS C 38, LEU C 60, ASN C 131, SER C 140, ASP C 141, GLU C 177, GLU C 178, LYS C 209, THR C 210, LYS C 214, GLN C 215, GLN C 226, GLU C 230, GLN C 233, LYS C 234, LYS C 241, ASP C 242, LYS C 269, ARG C 278, LYS C 305, LYS D 45, GLU D 49, LYS D 109, LYS D 137, LYS D 143, GLN D 158, LYS D 173, LYS D 181, LYS D 209, THR D 210, LYS D 211, LYS D 212, GLN D 215, ARG D 224, GLN D 226, GLU D 230, LYS D 234, LYS D 241, THR D 274, LYS D 305, LYS D 312 HAVE POORLY DEFINED ELECTRON DENSITY.
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Solvent computation | Solvent model: BABINET MODEL | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 24.104 Å2
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Refinement step | Cycle: LAST / Resolution: 2→48.17 Å
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Refine LS restraints |
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