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- PDB-4bzx: Structure of the Mycobacterium tuberculosis APS kinase CysC in co... -
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Open data
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Basic information
Entry | Database: PDB / ID: 4bzx | ||||||
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Title | Structure of the Mycobacterium tuberculosis APS kinase CysC in complex with AMPPNP and APS | ||||||
![]() | BIFUNCTIONAL ENZYME CYSN/CYSC | ||||||
![]() | TRANSFERASE / SULFUR ASSIMILATION | ||||||
Function / homology | ![]() sulfate adenylyltransferase complex (ATP) / cellular response to sulfur starvation / Sulfate assimilation / sulfate assimilation via adenylyl sulfate reduction / adenylyl-sulfate kinase / sulfate adenylyltransferase / adenylylsulfate kinase activity / sulfate adenylyltransferase (ATP) activity / sulfate assimilation / hydrogen sulfide biosynthetic process ...sulfate adenylyltransferase complex (ATP) / cellular response to sulfur starvation / Sulfate assimilation / sulfate assimilation via adenylyl sulfate reduction / adenylyl-sulfate kinase / sulfate adenylyltransferase / adenylylsulfate kinase activity / sulfate adenylyltransferase (ATP) activity / sulfate assimilation / hydrogen sulfide biosynthetic process / translation elongation factor activity / cellular response to oxidative stress / phosphorylation / GTPase activity / GTP binding / ATP binding / plasma membrane / cytosol Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Poyraz, O. / Lohkamp, B. / Schnell, R. / Schneider, G. | ||||||
![]() | ![]() Title: Crystal Structures of the Kinase Domain of the Sulfate-Activating Complex in Mycobacterium Tuberculosis. Authors: Poyraz, O. / Brunner, K. / Lohkamp, B. / Axelsson, H. / Hammarstrom, L.G.J. / Schnell, R. / Schneider, G. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 162.9 KB | Display | ![]() |
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PDB format | ![]() | 128.2 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 1.7 MB | Display | ![]() |
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Full document | ![]() | 1.7 MB | Display | |
Data in XML | ![]() | 20.2 KB | Display | |
Data in CIF | ![]() | 27.1 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 4bzpSC ![]() 4bzqC ![]() 4rfvC S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS domain:
NCS domain segments: Component-ID: _ / Ens-ID: 1 / Beg auth comp-ID: PRO / Beg label comp-ID: PRO / End auth comp-ID: SER / End label comp-ID: SER / Refine code: _ / Auth seq-ID: 440 - 612 / Label seq-ID: 1 - 173
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Components
-Protein , 1 types, 2 molecules AB
#1: Protein | Mass: 19057.648 Da / Num. of mol.: 2 / Fragment: RESIDUES 440-612 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() References: UniProt: Q10600, UniProt: P9WNM5*PLUS, adenylyl-sulfate kinase |
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-Non-polymers , 7 types, 195 molecules ![](data/chem/img/ADX.gif)
![](data/chem/img/ANP.gif)
![](data/chem/img/EDO.gif)
![](data/chem/img/PEG.gif)
![](data/chem/img/CIT.gif)
![](data/chem/img/MG.gif)
![](data/chem/img/HOH.gif)
![](data/chem/img/ANP.gif)
![](data/chem/img/EDO.gif)
![](data/chem/img/PEG.gif)
![](data/chem/img/CIT.gif)
![](data/chem/img/MG.gif)
![](data/chem/img/HOH.gif)
#2: Chemical | #3: Chemical | #4: Chemical | ChemComp-EDO / #5: Chemical | ChemComp-PEG / | #6: Chemical | #7: Chemical | #8: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.06 Å3/Da / Density % sol: 40.23 % / Description: NONE |
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Crystal grow | Details: 0.1 M CITRATE, PH 5.3 20% PEG4, 10% 2- PROPANOL, 1.9 MM APS, 2.3 MM AMPPNP, 1.8 MM MGCL2 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Date: Apr 11, 2011 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9793 Å / Relative weight: 1 |
Reflection | Resolution: 1.7→49.74 Å / Num. obs: 38074 / % possible obs: 96.6 % / Observed criterion σ(I): 2 / Redundancy: 4.3 % / Rmerge(I) obs: 0.07 / Net I/σ(I): 11.3 |
Reflection shell | Resolution: 1.7→1.79 Å / Redundancy: 3.7 % / Rmerge(I) obs: 0.52 / Mean I/σ(I) obs: 2.3 / % possible all: 83.3 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 4BZP Resolution: 1.7→52.26 Å / Cor.coef. Fo:Fc: 0.967 / Cor.coef. Fo:Fc free: 0.95 / SU B: 4.685 / SU ML: 0.081 / Cross valid method: THROUGHOUT / ESU R: 0.112 / ESU R Free: 0.112 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.7→52.26 Å
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Refine LS restraints |
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