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- PDB-4bxu: Structure of Pex14 in complex with Pex5 LVxEF motif -

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Basic information

Entry
Database: PDB / ID: 4bxu
TitleStructure of Pex14 in complex with Pex5 LVxEF motif
Components
  • PEROXISOMAL MEMBRANE PROTEIN PEX14
  • PEROXISOMAL TARGETING SIGNAL 1 RECEPTOR
KeywordsPROTEIN TRANSPORT / TRANSLOCATION
Function / homology
Function and homology information


peroxisome transport along microtubule / protein import into peroxisome matrix, substrate release / protein import into peroxisome matrix, translocation / peroxisome membrane targeting sequence binding / protein import into peroxisome membrane / peroxisome targeting sequence binding / protein targeting to peroxisome / peroxisome matrix targeting signal-1 binding / peroxisomal importomer complex / microtubule anchoring ...peroxisome transport along microtubule / protein import into peroxisome matrix, substrate release / protein import into peroxisome matrix, translocation / peroxisome membrane targeting sequence binding / protein import into peroxisome membrane / peroxisome targeting sequence binding / protein targeting to peroxisome / peroxisome matrix targeting signal-1 binding / peroxisomal importomer complex / microtubule anchoring / protein import into peroxisome matrix, receptor recycling / protein import into peroxisome matrix / protein import into peroxisome matrix, docking / Class I peroxisomal membrane protein import / protein carrier chaperone / very long-chain fatty acid metabolic process / cerebral cortex neuron differentiation / peroxisome organization / cell development / pexophagy / positive regulation of multicellular organism growth / endoplasmic reticulum organization / mitochondrial membrane organization / peroxisomal membrane / neuromuscular process / beta-tubulin binding / fatty acid beta-oxidation / cerebral cortex cell migration / peroxisomal matrix / protein transmembrane transporter activity / negative regulation of protein-containing complex assembly / Pexophagy / negative regulation of protein binding / Peroxisomal protein import / protein tetramerization / neuron migration / negative regulation of DNA-binding transcription factor activity / fibrillar center / small GTPase binding / cellular response to reactive oxygen species / transcription corepressor activity / peroxisome / E3 ubiquitin ligases ubiquitinate target proteins / protein-macromolecule adaptor activity / microtubule binding / protein-containing complex assembly / signaling receptor binding / negative regulation of DNA-templated transcription / Golgi apparatus / enzyme binding / protein-containing complex / mitochondrion / identical protein binding / membrane / nucleus / cytoplasm / cytosol
Similarity search - Function
Peroxisome membrane anchor protein Pex14p, N-terminal / Peroxisomal membrane protein 14 / Pex14 N-terminal domain / PEX5/PEX5L / Tetratricopeptide repeat / Tetratricopeptide repeat / TPR repeat region circular profile. / TPR repeat profile. / Tetratricopeptide repeats / Tetratricopeptide repeat ...Peroxisome membrane anchor protein Pex14p, N-terminal / Peroxisomal membrane protein 14 / Pex14 N-terminal domain / PEX5/PEX5L / Tetratricopeptide repeat / Tetratricopeptide repeat / TPR repeat region circular profile. / TPR repeat profile. / Tetratricopeptide repeats / Tetratricopeptide repeat / Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain / Tetratricopeptide-like helical domain superfamily / Arc Repressor Mutant, subunit A / Winged helix-like DNA-binding domain superfamily / Orthogonal Bundle / Mainly Alpha
Similarity search - Domain/homology
Peroxisomal membrane protein PEX14 / Peroxisomal targeting signal 1 receptor
Similarity search - Component
Biological speciesHOMO SAPIENS (human)
MethodSOLUTION NMR
AuthorsKooshapur, H. / Meyer, H.N. / Madl, T. / Sattler, M.
CitationJournal: J.Biol.Chem. / Year: 2014
Title: A Novel Pex14 Interacting Site of Human Pex5 is Critical for Matrix Protein Import Into Peroxisomes.
Authors: Neuhaus, A. / Kooshapur, H. / Wolf, J. / Meyer, H.N. / Madl, T. / Saidowsky, J. / Hambruch, E. / Lassam, A. / Jung, M. / Sattler, M. / Schliebs, W. / Erdmann, R.
History
DepositionJul 15, 2013Deposition site: PDBE / Processing site: PDBE
Revision 1.0Nov 27, 2013Provider: repository / Type: Initial release
Revision 1.1Jan 15, 2014Group: Database references
Revision 2.0May 15, 2024Group: Advisory / Atomic model ...Advisory / Atomic model / Data collection / Database references / Other
Category: atom_site / chem_comp_atom ...atom_site / chem_comp_atom / chem_comp_bond / database_2 / pdbx_database_status / pdbx_nmr_spectrometer / pdbx_validate_close_contact
Item: _atom_site.B_iso_or_equiv / _atom_site.Cartn_x ..._atom_site.B_iso_or_equiv / _atom_site.Cartn_x / _atom_site.Cartn_y / _atom_site.Cartn_z / _atom_site.auth_atom_id / _atom_site.label_atom_id / _database_2.pdbx_DOI / _database_2.pdbx_database_accession / _pdbx_database_status.status_code_cs / _pdbx_database_status.status_code_mr / _pdbx_nmr_spectrometer.model / _pdbx_validate_close_contact.auth_atom_id_1

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Assembly

Deposited unit
A: PEROXISOMAL MEMBRANE PROTEIN PEX14
B: PEROXISOMAL TARGETING SIGNAL 1 RECEPTOR


Theoretical massNumber of molelcules
Total (without water)9,1612
Polymers9,1612
Non-polymers00
Water00
1


  • Idetical with deposited unit
  • defined by author&software
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
MethodPQS
NMR ensembles
DataCriteria
Number of conformers (submitted / calculated)10 / -
Representative

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Components

#1: Protein PEROXISOMAL MEMBRANE PROTEIN PEX14 / PTS1 RECEPTOR-DOCKING PROTEIN / PEROXIN-14 / PEROXISOMAL MEMBRANE ANCHOR PROTEIN PEX14


Mass: 7453.446 Da / Num. of mol.: 1 / Fragment: RESIDUES 16-80
Source method: isolated from a genetically manipulated source
Details: RESIDUES 12-15 (GAMA) OF PEX14 ORIGINATE FROM THE EXPRESSION VECTOR
Source: (gene. exp.) HOMO SAPIENS (human) / Production host: ESCHERICHIA COLI (E. coli) / Strain (production host): BL21(DE3) / References: UniProt: O75381
#2: Protein/peptide PEROXISOMAL TARGETING SIGNAL 1 RECEPTOR / PTS1 RECEPTOR / PTS1R / PTS1-BP / PEROXIN-5 / PEROXISOMAL C-TERMINAL TARGETING SIGNAL IMPORT ...PTS1 RECEPTOR / PTS1R / PTS1-BP / PEROXIN-5 / PEROXISOMAL C-TERMINAL TARGETING SIGNAL IMPORT RECEPTOR / PEROXISOME RECEPTOR 1 / PEX5


Mass: 1707.746 Da / Num. of mol.: 1 / Fragment: RESIDUES 57-71 / Source method: obtained synthetically / Source: (synth.) HOMO SAPIENS (human) / References: UniProt: P50542

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Experimental details

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Experiment

ExperimentMethod: SOLUTION NMR
NMR detailsText: NONE

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Sample preparation

Sample conditionspH: 6.5 / Temperature: 298.0 K

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NMR measurement

NMR spectrometer
TypeManufacturerModelField strength (MHz)Spectrometer-ID
Bruker AVANCEBrukerAVANCE5001
Bruker AVANCEBrukerAVANCE6002
Bruker AVANCEBrukerAVANCE9003

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Processing

NMR softwareName: ARIA1.2/CNS / Developer: JP LINGE, SI O DONOGHUE / Classification: refinement
NMR ensembleConformers submitted total number: 10

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