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Open data
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Basic information
Entry | Database: PDB / ID: 4bxn | ||||||
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Title | Eg5(WT) complex | ||||||
![]() | KINESIN-LIKE PROTEIN KIF11 | ||||||
![]() | MOTOR PROTEIN / MITOSIS | ||||||
Function / homology | ![]() spindle elongation / regulation of mitotic centrosome separation / Kinesins / plus-end-directed microtubule motor activity / mitotic centrosome separation / COPI-dependent Golgi-to-ER retrograde traffic / microtubule motor activity / kinesin complex / spindle organization / microtubule-based movement ...spindle elongation / regulation of mitotic centrosome separation / Kinesins / plus-end-directed microtubule motor activity / mitotic centrosome separation / COPI-dependent Golgi-to-ER retrograde traffic / microtubule motor activity / kinesin complex / spindle organization / microtubule-based movement / mitotic spindle assembly / MHC class II antigen presentation / mitotic spindle organization / mitotic spindle / spindle pole / spindle / mitotic cell cycle / microtubule binding / microtubule / cell division / protein kinase binding / protein-containing complex / ATP binding / membrane / nucleus / cytosol Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Talapatra, S.K. / Anthony, N.G. / Mackay, S.P. / Kozielski, F. | ||||||
![]() | ![]() Title: The Mitotic Kinesin Eg5 Overcomes Inhibition to the Phase I/II Clinical Candidate Sb743921 by an Allosteric Resistance Mechanism. Authors: Talapatra, S.K. / Anthony, N.G. / Mackay, S.P. / Kozielski, F. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 156.7 KB | Display | ![]() |
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PDB format | ![]() | 120.1 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 4a1zC ![]() 4a28C ![]() 4as7SC ![]() 4b7bC S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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2 | ![]()
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Unit cell |
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Components
-Protein , 1 types, 2 molecules AB
#1: Protein | Mass: 41055.582 Da / Num. of mol.: 2 / Fragment: MOTOR DOMAIN, RESIDUES 1-368 Source method: isolated from a genetically manipulated source Details: SB743921 AS A LIGAND / Source: (gene. exp.) ![]() ![]() ![]() |
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-Non-polymers , 5 types, 92 molecules ![](data/chem/img/ADP.gif)
![](data/chem/img/CD.gif)
![](data/chem/img/CL.gif)
![](data/chem/img/6LX.gif)
![](data/chem/img/HOH.gif)
![](data/chem/img/CD.gif)
![](data/chem/img/CL.gif)
![](data/chem/img/6LX.gif)
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#2: Chemical | #3: Chemical | ChemComp-CD / #4: Chemical | ChemComp-CL / #5: Chemical | #6: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.69 Å3/Da / Density % sol: 54.23 % / Description: NONE |
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Crystal grow | pH: 6.8 Details: 0.02 M CACL2, 0.02 M CADMIUM CHLORIDE, 0.02 M COBALT(II) CHLORIDE, 20% W/V PEG-3350, pH 6.8 |
-Data collection
Diffraction | Mean temperature: 277 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC CCD / Detector: CCD / Date: Apr 17, 2011 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.92 Å / Relative weight: 1 |
Reflection twin | Operator: -h,-k,l / Fraction: 0.5 |
Reflection | Resolution: 2.8→30 Å / Num. obs: 9633 / % possible obs: 99.4 % / Observed criterion σ(I): 2 / Redundancy: 6.5 % / Biso Wilson estimate: 83.8 Å2 / Rmerge(I) obs: 0.09 / Net I/σ(I): 17.6 |
Reflection shell | Resolution: 3→30 Å / Redundancy: 6 % / Rmerge(I) obs: 0.5 / Mean I/σ(I) obs: 2 / % possible all: 99.7 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 4AS7 Resolution: 2.793→27.935 Å / σ(F): 1.96 / Phase error: 34.63 / Stereochemistry target values: TWIN_LSQ_F Details: DISORDERED REGION EITHER DELETED OR MODELLED STEREOCHEMICALLY
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 71.9 Å2 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.793→27.935 Å
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Refine LS restraints |
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LS refinement shell |
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