+Open data
-Basic information
Entry | Database: PDB / ID: 4bxn | ||||||
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Title | Eg5(WT) complex | ||||||
Components | KINESIN-LIKE PROTEIN KIF11 | ||||||
Keywords | MOTOR PROTEIN / MITOSIS | ||||||
Function / homology | Function and homology information spindle elongation / Kinesins / plus-end-directed microtubule motor activity / regulation of mitotic centrosome separation / mitotic centrosome separation / COPI-dependent Golgi-to-ER retrograde traffic / kinesin complex / microtubule motor activity / spindle organization / microtubule-based movement ...spindle elongation / Kinesins / plus-end-directed microtubule motor activity / regulation of mitotic centrosome separation / mitotic centrosome separation / COPI-dependent Golgi-to-ER retrograde traffic / kinesin complex / microtubule motor activity / spindle organization / microtubule-based movement / mitotic spindle assembly / MHC class II antigen presentation / mitotic spindle organization / mitotic spindle / spindle / spindle pole / mitotic cell cycle / microtubule binding / microtubule / cell division / protein kinase binding / protein-containing complex / ATP binding / membrane / nucleus / cytosol Similarity search - Function | ||||||
Biological species | HOMO SAPIENS (human) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.793 Å | ||||||
Authors | Talapatra, S.K. / Anthony, N.G. / Mackay, S.P. / Kozielski, F. | ||||||
Citation | Journal: J.Med.Chem. / Year: 2013 Title: The Mitotic Kinesin Eg5 Overcomes Inhibition to the Phase I/II Clinical Candidate Sb743921 by an Allosteric Resistance Mechanism. Authors: Talapatra, S.K. / Anthony, N.G. / Mackay, S.P. / Kozielski, F. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 4bxn.cif.gz | 156.7 KB | Display | PDBx/mmCIF format |
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PDB format | pdb4bxn.ent.gz | 120.1 KB | Display | PDB format |
PDBx/mmJSON format | 4bxn.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/bx/4bxn ftp://data.pdbj.org/pub/pdb/validation_reports/bx/4bxn | HTTPS FTP |
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-Related structure data
Related structure data | 4a1zC 4a28C 4as7SC 4b7bC S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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2 |
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Unit cell |
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-Components
-Protein , 1 types, 2 molecules AB
#1: Protein | Mass: 41055.582 Da / Num. of mol.: 2 / Fragment: MOTOR DOMAIN, RESIDUES 1-368 Source method: isolated from a genetically manipulated source Details: SB743921 AS A LIGAND / Source: (gene. exp.) HOMO SAPIENS (human) / Plasmid: PPROEX / Production host: ESCHERICHIA COLI (E. coli) / Strain (production host): BL21(DE3) / Variant (production host): CODON PLUS / References: UniProt: P52732 |
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-Non-polymers , 5 types, 92 molecules
#2: Chemical | #3: Chemical | ChemComp-CD / #4: Chemical | ChemComp-CL / #5: Chemical | #6: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 20 |
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-Sample preparation
Crystal | Density Matthews: 2.69 Å3/Da / Density % sol: 54.23 % / Description: NONE |
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Crystal grow | pH: 6.8 Details: 0.02 M CACL2, 0.02 M CADMIUM CHLORIDE, 0.02 M COBALT(II) CHLORIDE, 20% W/V PEG-3350, pH 6.8 |
-Data collection
Diffraction | Mean temperature: 277 K |
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Diffraction source | Source: SYNCHROTRON / Site: Diamond / Beamline: I04-1 / Wavelength: 0.92 |
Detector | Type: ADSC CCD / Detector: CCD / Date: Apr 17, 2011 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.92 Å / Relative weight: 1 |
Reflection twin | Operator: -h,-k,l / Fraction: 0.5 |
Reflection | Resolution: 2.8→30 Å / Num. obs: 9633 / % possible obs: 99.4 % / Observed criterion σ(I): 2 / Redundancy: 6.5 % / Biso Wilson estimate: 83.8 Å2 / Rmerge(I) obs: 0.09 / Net I/σ(I): 17.6 |
Reflection shell | Resolution: 3→30 Å / Redundancy: 6 % / Rmerge(I) obs: 0.5 / Mean I/σ(I) obs: 2 / % possible all: 99.7 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PDB ENTRY 4AS7 Resolution: 2.793→27.935 Å / σ(F): 1.96 / Phase error: 34.63 / Stereochemistry target values: TWIN_LSQ_F Details: DISORDERED REGION EITHER DELETED OR MODELLED STEREOCHEMICALLY
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 71.9 Å2 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.793→27.935 Å
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Refine LS restraints |
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LS refinement shell |
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