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- PDB-4buj: Crystal structure of the S. cerevisiae Ski2-3-8 complex -

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Open data


ID or keywords:

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Basic information

Entry
Database: PDB / ID: 4buj
TitleCrystal structure of the S. cerevisiae Ski2-3-8 complex
Components
  • ANTIVIRAL HELICASE SKI2
  • ANTIVIRAL PROTEIN SKI8
  • SUPERKILLER PROTEIN 3
KeywordsHYDROLASE / DEXH BOX HELICASE / RNA DEGRADATION / TPR / PROTEIN COMPLEX
Function / homology
Function and homology information


mRNA decay by 3' to 5' exoribonuclease / protein-DNA complex assembly / Ski complex / nuclear-transcribed mRNA catabolic process, non-stop decay / nuclear-transcribed mRNA catabolic process, 3'-5' exonucleolytic nonsense-mediated decay / reciprocal meiotic recombination / nuclear chromosome / mRNA catabolic process / nuclear-transcribed mRNA catabolic process / regulation of translation ...mRNA decay by 3' to 5' exoribonuclease / protein-DNA complex assembly / Ski complex / nuclear-transcribed mRNA catabolic process, non-stop decay / nuclear-transcribed mRNA catabolic process, 3'-5' exonucleolytic nonsense-mediated decay / reciprocal meiotic recombination / nuclear chromosome / mRNA catabolic process / nuclear-transcribed mRNA catabolic process / regulation of translation / protein-containing complex assembly / defense response to virus / RNA helicase activity / RNA helicase / mRNA binding / ATP hydrolysis activity / DNA binding / ATP binding / nucleus / cytoplasm
Similarity search - Function
Tetratricopeptide-like repeat / Tetratricopeptide repeat / Sec63 N-terminal domain-like fold - #30 / : / Ski2, beta-barrel domain / Ski3/TTC37 / Sec63 N-terminal domain-like fold / Ski2, N-terminal domain / Ski2 N-terminal region / : ...Tetratricopeptide-like repeat / Tetratricopeptide repeat / Sec63 N-terminal domain-like fold - #30 / : / Ski2, beta-barrel domain / Ski3/TTC37 / Sec63 N-terminal domain-like fold / Ski2, N-terminal domain / Ski2 N-terminal region / : / ATP-dependent RNA helicase Ski2, C-terminal / ATP-dependent RNA helicase Ski2-like / : / DSHCT (NUC185) domain / Exosome RNA helicase MTR4-like, stalk / DSHCT / : / Tetratricopeptide repeat / TPR repeat region circular profile. / TPR repeat profile. / Tetratricopeptide repeats / DEAD/DEAH box helicase domain / DEAD/DEAH box helicase / YVTN repeat-like/Quinoprotein amine dehydrogenase / Tetratricopeptide repeat / 7 Propeller / Methylamine Dehydrogenase; Chain H / Helicase conserved C-terminal domain / Tetratricopeptide-like helical domain superfamily / helicase superfamily c-terminal domain / WD domain, G-beta repeat / Superfamilies 1 and 2 helicase C-terminal domain profile. / Superfamilies 1 and 2 helicase ATP-binding type-1 domain profile. / DEAD-like helicases superfamily / Helicase, C-terminal / Helicase superfamily 1/2, ATP-binding domain / P-loop containing nucleotide triphosphate hydrolases / WD40 repeat, conserved site / Trp-Asp (WD) repeats signature. / Trp-Asp (WD) repeats profile. / Trp-Asp (WD) repeats circular profile. / WD40 repeats / WD40 repeat / WD40-repeat-containing domain superfamily / WD40/YVTN repeat-like-containing domain superfamily / Rossmann fold / P-loop containing nucleoside triphosphate hydrolase / Orthogonal Bundle / 3-Layer(aba) Sandwich / Mainly Beta / Mainly Alpha / Alpha Beta
Similarity search - Domain/homology
Superkiller protein 3 / Antiviral helicase SKI2 / Antiviral protein SKI8
Similarity search - Component
Biological speciesSACCHAROMYCES CEREVISIAE (brewer's yeast)
MethodX-RAY DIFFRACTION / SYNCHROTRON / SAD / Resolution: 3.7 Å
AuthorsHalbach, F. / Reichelt, P. / Rode, M. / Conti, E.
CitationJournal: Cell(Cambridge,Mass.) / Year: 2013
Title: The Yeast Ski Complex: Crystal Structure and RNA Channeling to the Exosome Complex.
Authors: Halbach, F. / Reichelt, P. / Rode, M. / Conti, E.
History
DepositionJun 20, 2013Deposition site: PDBE / Processing site: PDBE
Revision 1.0Aug 28, 2013Provider: repository / Type: Initial release
Revision 1.1Nov 6, 2019Group: Data collection / Other / Refinement description / Category: pdbx_database_status / refine
Item: _pdbx_database_status.status_code_sf / _refine.pdbx_method_to_determine_struct
Revision 1.2Dec 20, 2023Group: Data collection / Database references ...Data collection / Database references / Derived calculations / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model / struct_site
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession ..._database_2.pdbx_DOI / _database_2.pdbx_database_accession / _struct_site.pdbx_auth_asym_id / _struct_site.pdbx_auth_comp_id / _struct_site.pdbx_auth_seq_id
Remark 650 HELIX DETERMINATION METHOD: AUTHOR PROVIDED.

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: ANTIVIRAL HELICASE SKI2
B: SUPERKILLER PROTEIN 3
C: ANTIVIRAL PROTEIN SKI8
D: ANTIVIRAL PROTEIN SKI8
E: ANTIVIRAL HELICASE SKI2
F: SUPERKILLER PROTEIN 3
G: ANTIVIRAL PROTEIN SKI8
H: ANTIVIRAL PROTEIN SKI8
hetero molecules


Theoretical massNumber of molelcules
Total (without water)738,24728
Polymers736,3268
Non-polymers1,92120
Water00
1
A: ANTIVIRAL HELICASE SKI2
B: SUPERKILLER PROTEIN 3
C: ANTIVIRAL PROTEIN SKI8
D: ANTIVIRAL PROTEIN SKI8
hetero molecules


Theoretical massNumber of molelcules
Total (without water)369,22015
Polymers368,1634
Non-polymers1,05711
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
MethodPISA
2
E: ANTIVIRAL HELICASE SKI2
F: SUPERKILLER PROTEIN 3
G: ANTIVIRAL PROTEIN SKI8
H: ANTIVIRAL PROTEIN SKI8
hetero molecules


Theoretical massNumber of molelcules
Total (without water)369,02813
Polymers368,1634
Non-polymers8659
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
MethodPISA
Unit cell
Length a, b, c (Å)183.375, 200.415, 340.179
Angle α, β, γ (deg.)90.00, 90.00, 90.00
Int Tables number19
Space group name H-MP212121
Noncrystallographic symmetry (NCS)NCS oper:
IDCodeMatrixVector
1given(-0.006525, -0.9902, -0.1394), (-0.9893, -0.01391, 0.1451), (-0.1456, 0.1389, -0.9795)-46.94, -60.11, 85.54
2given(-0.01979, -0.9962, -0.08534), (-0.9868, 0.005729, 0.1619), (-0.1608, 0.08742, -0.9831)-50.47, -59.98, 83.21
3given(-0.01483, -0.9908, -0.1345), (-0.9901, -0.004221, 0.1403), (-0.1396, 0.1352, -0.9809)-47.86, -59.61, 86.02
4given(-0.02875, -0.9901, -0.1372), (-0.9875, 0.006868, 0.1573), (-0.1548, 0.1401, -0.978)-48.96, -60.48, 84.5

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Components

#1: Protein ANTIVIRAL HELICASE SKI2 / SUPERKILLER PROTEIN 2 / SKI2


Mass: 115317.445 Da / Num. of mol.: 2 / Fragment: RESIDUES 1-834,1086-1287
Source method: isolated from a genetically manipulated source
Details: RESIDUES 835-1085 REPLACED BY A GLY-SER-ARG-GLY LINKER
Source: (gene. exp.) SACCHAROMYCES CEREVISIAE (brewer's yeast)
Strain: S288C / Plasmid: PFASTBAC 1 / Production host: TRICHOPLUSIA NI (cabbage looper) / References: UniProt: P35207, RNA helicase
#2: Protein SUPERKILLER PROTEIN 3 / SKI3


Mass: 164278.484 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) SACCHAROMYCES CEREVISIAE (brewer's yeast)
Strain: S288C / Plasmid: PFL / Production host: TRICHOPLUSIA NI (cabbage looper) / References: UniProt: P17883
#3: Protein
ANTIVIRAL PROTEIN SKI8 / SUPERKILLER PROTEIN 8 / SKI8


Mass: 44283.527 Da / Num. of mol.: 4
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) SACCHAROMYCES CEREVISIAE (brewer's yeast)
Strain: S288C / Plasmid: PFL / Production host: TRICHOPLUSIA NI (cabbage looper) / References: UniProt: Q02793
#4: Chemical
ChemComp-SO4 / SULFATE ION


Mass: 96.063 Da / Num. of mol.: 20 / Source method: obtained synthetically / Formula: SO4
Sequence detailsCHAINS A,E: RESIDUES 835-1085 OF SKI2 (UNP P35207) REPLACED BY A GLY-SER-ARG-GLY LINKER DUE TO WEAK ...CHAINS A,E: RESIDUES 835-1085 OF SKI2 (UNP P35207) REPLACED BY A GLY-SER-ARG-GLY LINKER DUE TO WEAK DENSITY, RESIDUES 218 - 281 (CHAIN A) AND 219 - 281 (CHAIN E) WERE MODELED AS UNKNOWN RESIDUES (UNK)

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 4.25 Å3/Da / Density % sol: 71.1 % / Description: NONE
Crystal growpH: 6 / Details: 1.88 M AMMONIUM SULFATE, 100 MM BISTRIS PH 6.0

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Data collection

DiffractionMean temperature: 100 K
Diffraction sourceSource: SYNCHROTRON / Site: SLS / Beamline: X10SA / Wavelength: 0.9788
DetectorType: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Sep 23, 2010
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.9788 Å / Relative weight: 1
ReflectionResolution: 3.7→96 Å / Num. obs: 132568 / % possible obs: 99.1 % / Observed criterion σ(I): 1 / Redundancy: 3.3 % / Rmerge(I) obs: 0.14 / Net I/σ(I): 5.8
Reflection shellResolution: 3.7→3.9 Å / Redundancy: 3.3 % / Rmerge(I) obs: 0.91 / Mean I/σ(I) obs: 1.4 / % possible all: 99.2

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Processing

Software
NameVersionClassification
PHENIX(PHENIX.REFINE)refinement
XDSdata reduction
SCALAdata scaling
PHASERphasing
RefinementMethod to determine structure: SAD
Starting model: PDB ENTRIES 1S4U, 4A4Z

1s4u

4a4z


Resolution: 3.7→96.124 Å / SU ML: 0.54 / σ(F): 0 / Phase error: 25.82 / Stereochemistry target values: ML
RfactorNum. reflection% reflection
Rfree0.2649 6666 5 %
Rwork0.2308 --
obs0.2325 132408 98.82 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso mean: 139.86 Å2
Refinement stepCycle: LAST / Resolution: 3.7→96.124 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms42919 0 100 0 43019
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.00543880
X-RAY DIFFRACTIONf_angle_d0.80559970
X-RAY DIFFRACTIONf_dihedral_angle_d12.81214160
X-RAY DIFFRACTIONf_chiral_restr0.0457039
X-RAY DIFFRACTIONf_plane_restr0.0037763
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
3.7-3.83230.34436650.331812554X-RAY DIFFRACTION99
3.8323-3.98570.3236340.293112440X-RAY DIFFRACTION99
3.9857-4.16710.29916690.26812469X-RAY DIFFRACTION99
4.1671-4.38680.27297110.241312529X-RAY DIFFRACTION100
4.3868-4.66160.25386610.223512552X-RAY DIFFRACTION99
4.6616-5.02150.25546530.215612521X-RAY DIFFRACTION99
5.0215-5.52680.28826400.23712608X-RAY DIFFRACTION99
5.5268-6.32640.29056850.255912613X-RAY DIFFRACTION99
6.3264-7.97010.25036710.221912695X-RAY DIFFRACTION99
7.9701-96.15620.23126770.194612761X-RAY DIFFRACTION97
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
10.57860.83621.25873.4445-0.7647.7561-0.36990.00690.887-0.0737-0.3706-0.46550.2270.36630.34360.5966-0.0794-0.06810.7506-0.09911.0894-61.95198.194868.1701
22.5202-2.97530.725.0998-0.65920.93330.52570.63620.2995-1.0003-0.6264-0.13860.0888-0.02820.19860.8341-0.04430.09090.72610.22630.6663-70.6297-31.762840.5996
32.37180.25572.3850.10630.47444.2337-0.4370.1575-0.7415-0.288-0.09730.53810.0386-0.00510.2141.0776-0.09490.02080.5911-0.1911.0897-75.6619-60.482645.6582
41.5193-0.93450.10840.3748-0.16961.34880.01870.1443-0.2758-0.109-0.06470.22270.68371.1014-0.3990.95620.27490.1760.5289-0.47981.4664-45.291-81.328942.9357
52.6969-0.32340.60214.6874-0.24012.8998-0.1366-0.4842-0.20450.35810.08590.02430.11080.14840.08850.6581-0.07430.1140.61620.15450.6498-60.4752-60.942778.2393
61.0725-0.38970.8443.8048-0.99932.29620.2055-0.3903-0.13090.6943-0.23770.3030.29110.06930.06021.087-0.0220.28480.98610.07930.6107-71.6505-49.9845104.3884
73.0856-0.82740.25881.95640.6761.2145-0.1325-0.1347-0.58010.0235-0.03540.41250.2515-0.17090.16150.7083-0.15810.22020.55450.02950.8312-88.2884-47.491471.6209
81.0218-0.4334-1.56661.8661.07273.1748-0.4008-0.18940.12270.4088-0.01590.47220.7254-0.62840.33831.2228-0.3085-0.14671.95840.09930.7384-67.7658-112.0827156.4374
92.78680.043-2.1560.15140.14233.5659-0.2933-0.80280.31350.32340.41450.10750.52760.1704-0.16961.1192-0.02140.00811.43590.03540.7324-41.8785-109.6485114.428
102.0982-0.7883-1.47412.0473-1.37363.82410.38860.31990.1746-0.1883-0.2818-0.19750.26660.1508-0.1230.62780.0948-0.16880.97450.06120.8984-23.8783-94.488786.7439
111.6782-2.82180.37584.1743-0.55883.16630.26130.24210.3023-0.1565-0.3517-0.51640.43170.55130.05580.72010.10080.22050.94020.1740.716-36.8112-68.247457.6608
122.42451.3333-0.90411.3171-0.13061.7097-0.18060.38890.0975-0.81380.00340.20750.2175-0.20120.18391.04480.1128-0.05750.7058-0.03840.4988-65.7808-48.723931.9439
132.5305-0.71290.4233.0634-1.86592.23230.29040.455-0.1531-0.22340.03430.21470.0635-0.0771-0.3340.6730.0276-0.13020.9021-0.00850.9359-81.3163-18.561633.9289
142.1113-0.54180.59691.63650.37832.38830.0630.11340.5352-0.3958-0.05940.2788-0.7336-0.49090.00460.87960.1033-0.0240.60790.04411.0482-89.83513.805454.649
152.3766-0.2285-1.13661.8898-0.25773.60180.0044-0.56730.12670.2322-0.2006-0.3458-0.10240.67120.11390.5816-0.2045-0.06450.59890.08770.7603-49.1323-10.75667.5534
163.93411.5201-0.91495.1556-0.85315.7721-0.2350.39120.4206-0.55990.46490.86240.1378-0.3936-0.05360.5672-0.06270.05060.47240.13440.6835-66.147-20.087455.0158
173.04570.7099-1.48742.94080.17682.9414-0.1669-0.46680.04240.4864-0.1799-0.05990.4711-0.05660.31430.5745-0.0803-0.01980.58410.050.5645-64.9803-22.32474.8789
182.8948-0.05990.6262.471-0.00723.9993-0.2443-0.66090.50950.5567-0.08740.0676-0.2771-0.14840.33940.75410.07490.10990.6756-0.28081.0171-101.12431.194187.5589
195.1525-1.168-0.24774.6042-3.32078.9895-0.6886-0.1936-0.8047-0.0987-0.1021-0.31471.4998-0.83180.83030.79720.0110.1680.6032-0.11551.2416-100.8117-17.945678.5481
202.2692-0.878-0.40523.3759-0.27442.0814-0.01540.04620.035-0.4623-0.31260.5972-0.5258-0.7180.27170.70370.03950.08210.7674-0.3621.2493-116.0965-7.749772.3834
213.5140.41970.38799.67951.33682.4580.19620.36890.3708-0.8069-0.24771.4259-0.1304-0.89440.29430.6221-0.0605-0.2341.29180.43181.3844-64.160110.742428.549
225.2758-1.6742-0.55292.63221.14040.7126-0.395-0.27731.55610.50870.2334-0.35470.23990.08660.09281.0459-0.04150.1610.92930.25040.522-21.695914.798250.2786
232.7690.90220.58455.26964.37753.65620.8768-0.05040.94030.161-1.2402-1.4257-0.65310.26750.54690.8066-0.3754-0.33771.72010.63931.71954.874117.017536.174
240.6739-0.16760.91861.28851.00572.50880.50840.24060.285-0.5896-0.4864-0.5380.90870.7648-0.6398-0.2512-0.66630.10431.21480.45391.023118.28955.626434.0935
251.62310.0024-0.45642.4372-0.69611.85830.29791.53191.0253-0.2243-0.5293-0.98070.06360.46280.33441.1168-0.18570.02131.87260.73761.24431.66313.095310.8376
260.0048-0.1288-0.02390.8398-0.5361.3240.17491.19350.2712-0.4943-0.1219-0.43080.15750.31330.50581.3815-0.17730.19243.15441.41251.483-10.776226.4444-12.0364
270.8649-0.27150.451.44350.46450.4353-0.21180.71081.2462-0.19130.0699-0.5427-0.24320.31850.06831.2721-0.5487-0.16821.62830.78782.1087-10.145338.453221.8496
282.2133-0.1488-0.70421.0411-1.75053.47980.2724-0.22860.48680.5807-0.41760.2324-1.4294-0.16530.15681.9697-0.0818-0.11091.1699-0.30220.825557.00327.2837-70.9043
291.27051.0854-0.61091.0828-0.0791.29150.320.21920.1926-0.2095-0.09440.1620.3675-0.1267-0.16751.53310.0387-0.00611.36880.03440.727843.2847-8.2875-21.8011
302.5007-1.0122-1.55582.02890.57232.8001-0.3489-0.0054-0.1443-0.00690.5854-0.36880.91910.0676-0.16511.14030.1611-0.02941.2121-0.14290.664520.9306-20.261318.9349
311.64840.6362-1.123.11870.86832.9497-0.096-0.2390.1583-0.20.12610.32790.39020.2382-0.11130.57530.0667-0.09521.13830.1620.5852-7.6336-2.012154.9349
322.674-0.7465-0.19241.7444-0.90772.38950.0651-0.57590.61620.1399-0.12530.2236-0.1350.3485-0.0260.5631-0.1505-0.08241.41830.08261.163-0.690521.703256.1575
333.1548-1.9278-0.49941.87960.67733.3972-0.3202-0.04610.91610.08540.295-0.01930.0249-0.5107-0.04240.7308-0.0741-0.07380.8398-0.16431.1955-38.052926.418764.0385
342.9088-0.7353-0.47390.85180.02942.6725-0.0075-0.34431.2939-0.07850.01840.0561-0.8183-0.39650.0430.98050.1602-0.20970.96660.05511.8033-58.380936.463241.3983
353.6975-0.41570.12373.08780.27523.0466-0.10330.77210.152-0.6440.13950.03280.4686-0.1185-0.07760.5793-0.15340.00130.71250.1820.5651-45.6648-1.171624.9281
362.40411.7855-0.30544.8388-1.74253.13380.0152-0.18420.45211.003-0.77790.1661-0.20390.75570.55540.5765-0.14420.03810.79010.43951.1151-37.26128.170741.8366
372.9791.0884-1.53732.4973-1.32663.2429-0.0820.66120.87-0.2307-0.2577-0.1648-0.34430.28070.18940.7556-0.1873-0.17430.99950.43671.2234-33.835415.715123.9229
382.8173-0.4438-0.89411.7555-0.33971.8739-0.33371.02211.6877-0.5529-0.06960.8982-0.9642-0.13520.32291.69210.2864-0.50021.43281.09533.127-59.560153.39714.4949
391.39430.4799-0.2930.3906-0.42810.79310.31840.12042.0940.2056-0.3404-0.787-1.05840.2819-0.60421.7804-0.2593-0.18060.92240.87893.3517-41.64159.019525.8357
404.4438-0.0679-0.95947.24131.36790.49390.2005-0.3770.3465-0.4975-0.751-0.2051-0.29630.04840.57491.62180.1512-0.0981.30780.61942.9522-58.193668.617124.5659
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection details
1X-RAY DIFFRACTION1(CHAIN A AND RESID 8:39)
2X-RAY DIFFRACTION2(CHAIN A AND RESID 45:121)
3X-RAY DIFFRACTION3(CHAIN A AND RESID 122:222)
4X-RAY DIFFRACTION4(CHAIN A AND RESID 223:302)
5X-RAY DIFFRACTION5(CHAIN A AND RESID 303:505)
6X-RAY DIFFRACTION6(CHAIN A AND RESID 506:797)
7X-RAY DIFFRACTION7(CHAIN A AND RESID 798:1287)
8X-RAY DIFFRACTION8(CHAIN B AND RESID -1:168)
9X-RAY DIFFRACTION9(CHAIN B AND RESID 169:401)
10X-RAY DIFFRACTION10(CHAIN B AND RESID 402:613)
11X-RAY DIFFRACTION11(CHAIN B AND RESID 614:802)
12X-RAY DIFFRACTION12(CHAIN B AND RESID 803:1067)
13X-RAY DIFFRACTION13(CHAIN B AND RESID 1068:1238)
14X-RAY DIFFRACTION14(CHAIN B AND RESID 1239:1432)
15X-RAY DIFFRACTION15(CHAIN C AND RESID 2:212)
16X-RAY DIFFRACTION16(CHAIN C AND RESID 213:301)
17X-RAY DIFFRACTION17(CHAIN C AND RESID 302:397)
18X-RAY DIFFRACTION18(CHAIN D AND RESID 3:194)
19X-RAY DIFFRACTION19(CHAIN D AND RESID 195:246)
20X-RAY DIFFRACTION20(CHAIN D AND RESID 247:397)
21X-RAY DIFFRACTION21(CHAIN E AND RESID 9:44)
22X-RAY DIFFRACTION22(CHAIN E AND RESID 45:121)
23X-RAY DIFFRACTION23(CHAIN E AND RESID 122:186)
24X-RAY DIFFRACTION24(CHAIN E AND RESID 187:326)
25X-RAY DIFFRACTION25(CHAIN E AND RESID 327:497)
26X-RAY DIFFRACTION26(CHAIN E AND RESID 498:801)
27X-RAY DIFFRACTION27(CHAIN E AND RESID 802:1287)
28X-RAY DIFFRACTION28(CHAIN F AND RESID -1:168)
29X-RAY DIFFRACTION29(CHAIN F AND RESID 169:540)
30X-RAY DIFFRACTION30(CHAIN F AND RESID 541:779)
31X-RAY DIFFRACTION31(CHAIN F AND RESID 780:934)
32X-RAY DIFFRACTION32(CHAIN F AND RESID 935:1089)
33X-RAY DIFFRACTION33(CHAIN F AND RESID 1090:1269)
34X-RAY DIFFRACTION34(CHAIN F AND RESID 1270:1432)
35X-RAY DIFFRACTION35(CHAIN G AND RESID 2:208)
36X-RAY DIFFRACTION36(CHAIN G AND RESID 209:255)
37X-RAY DIFFRACTION37(CHAIN G AND RESID 256:397)
38X-RAY DIFFRACTION38(CHAIN H AND RESID 3:188)
39X-RAY DIFFRACTION39(CHAIN H AND RESID 189:331)
40X-RAY DIFFRACTION40(CHAIN H AND RESID 332:394)

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