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Yorodumi- PDB-4bkk: The Respiratory Syncytial Virus nucleoprotein-RNA complex forms a... -
+Open data
-Basic information
Entry | Database: PDB / ID: 4bkk | ||||||
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Title | The Respiratory Syncytial Virus nucleoprotein-RNA complex forms a left-handed helical nucleocapsid. | ||||||
Components |
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Keywords | VIRAL PROTEIN/RNA / VIRAL PROTEIN-RNA COMPLEX | ||||||
Function / homology | Function and homology information Respiratory syncytial virus genome transcription / Translation of respiratory syncytial virus mRNAs / symbiont-mediated suppression of host PKR/eIFalpha signaling / protein serine/threonine kinase inhibitor activity / Respiratory syncytial virus genome replication / helical viral capsid / RSV-host interactions / Assembly and release of respiratory syncytial virus (RSV) virions / Maturation of hRSV A proteins / Respiratory syncytial virus (RSV) attachment and entry ...Respiratory syncytial virus genome transcription / Translation of respiratory syncytial virus mRNAs / symbiont-mediated suppression of host PKR/eIFalpha signaling / protein serine/threonine kinase inhibitor activity / Respiratory syncytial virus genome replication / helical viral capsid / RSV-host interactions / Assembly and release of respiratory syncytial virus (RSV) virions / Maturation of hRSV A proteins / Respiratory syncytial virus (RSV) attachment and entry / symbiont-mediated suppression of host cytoplasmic pattern recognition receptor signaling pathway via inhibition of MDA-5 activity / symbiont-mediated suppression of host cytoplasmic pattern recognition receptor signaling pathway via inhibition of MAVS activity / PKR-mediated signaling / Evasion by RSV of host interferon responses / viral capsid / symbiont-mediated suppression of host NF-kappaB cascade / viral nucleocapsid / host cell cytoplasm / symbiont-mediated suppression of host type I interferon-mediated signaling pathway / ribonucleoprotein complex / virus-mediated perturbation of host defense response / RNA binding Similarity search - Function | ||||||
Biological species | HUMAN RESPIRATORY SYNCYTIAL VIRUS A STRAIN LONG | ||||||
Method | ELECTRON MICROSCOPY / electron tomography / negative staining | ||||||
Authors | Bakker, S.E. / Duquerroy, S. / Galloux, M. / Loney, C. / Conner, E. / Eleouet, J.F. / Rey, F.A. / Bhella, D. | ||||||
Citation | Journal: J Gen Virol / Year: 2013 Title: The respiratory syncytial virus nucleoprotein-RNA complex forms a left-handed helical nucleocapsid. Authors: Saskia E Bakker / Stéphane Duquerroy / Marie Galloux / Colin Loney / Edward Conner / Jean-François Eléouët / Félix A Rey / David Bhella / Abstract: Respiratory syncytial virus (RSV) is an important human pathogen. Its nucleocapsid (NC), which comprises the negative sense RNA viral genome coated by the viral nucleoprotein N, is a critical ...Respiratory syncytial virus (RSV) is an important human pathogen. Its nucleocapsid (NC), which comprises the negative sense RNA viral genome coated by the viral nucleoprotein N, is a critical assembly that serves as template for both mRNA synthesis and genome replication. We have previously described the X-ray structure of an NC-like structure: a decameric ring formed of N-RNA that mimics one turn of the helical NC. In the absence of experimental data we had hypothesized that the NC helix would be right-handed, as the N-N contacts in the ring appeared to more easily adapt to that conformation. We now unambiguously show that the RSV NC is a left-handed helix. We further show that the contacts in the ring can be distorted to maintain key N-N-protein interactions in a left-handed helix, and discuss the implications of the resulting atomic model of the helical NC for viral replication and transcription. | ||||||
History |
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-Structure visualization
Movie |
Movie viewer |
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Structure viewer | Molecule: MolmilJmol/JSmol |
-Downloads & links
-Download
PDBx/mmCIF format | 4bkk.cif.gz | 1.6 MB | Display | PDBx/mmCIF format |
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PDB format | pdb4bkk.ent.gz | 1.3 MB | Display | PDB format |
PDBx/mmJSON format | 4bkk.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 4bkk_validation.pdf.gz | 451.8 KB | Display | wwPDB validaton report |
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Full document | 4bkk_full_validation.pdf.gz | 512 KB | Display | |
Data in XML | 4bkk_validation.xml.gz | 167.8 KB | Display | |
Data in CIF | 4bkk_validation.cif.gz | 281.7 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/bk/4bkk ftp://data.pdbj.org/pub/pdb/validation_reports/bk/4bkk | HTTPS FTP |
-Related structure data
Related structure data | 2369MC 2370C M: map data used to model this data C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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-Components
#1: RNA chain | Mass: 49089.227 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) HUMAN RESPIRATORY SYNCYTIAL VIRUS A STRAIN LONG Cell line (production host): Sf21 / Production host: SPODOPTERA FRUGIPERDA (fall armyworm) |
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#2: Protein | Mass: 43507.848 Da / Num. of mol.: 23 Source method: isolated from a genetically manipulated source Source: (gene. exp.) HUMAN RESPIRATORY SYNCYTIAL VIRUS A STRAIN LONG Cell line (production host): Sf21 / Production host: SPODOPTERA FRUGIPERDA (fall armyworm) / References: UniProt: P03418 |
-Experimental details
-Experiment
Experiment | Method: ELECTRON MICROSCOPY |
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EM experiment | Aggregation state: FILAMENT / 3D reconstruction method: electron tomography |
-Sample preparation
Component | Name: PURIFIED RECOMBINANT RSV NUCLEOCAPSIDS. / Type: VIRUS |
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Buffer solution | Name: 50 MM TRIS-HCL, PH 7.4, 150 MM NACL, 10 MM EDTA / pH: 7.4 / Details: 50 MM TRIS-HCL, PH 7.4, 150 MM NACL, 10 MM EDTA |
Specimen | Embedding applied: NO / Shadowing applied: NO / Staining applied: YES / Vitrification applied: NO |
EM staining | Type: NEGATIVE / Material: Ammonium Molybdate |
Specimen support | Details: HOLEY CARBON |
-Electron microscopy imaging
Microscopy | Model: JEOL 2200FSC / Date: Jul 15, 2010 Details: ROOM TEMPERATURE STAINED GRIDS IMAGED AT LN2 TEMPERATURES. |
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Electron gun | Electron source: FIELD EMISSION GUN / Accelerating voltage: 200 kV / Illumination mode: FLOOD BEAM |
Electron lens | Mode: BRIGHT FIELD / Nominal magnification: 40000 X / Nominal defocus max: 4000 nm / Nominal defocus min: 2000 nm / Cs: 2 mm |
Specimen holder | Tilt angle max: 60 ° / Tilt angle min: -60 ° |
Image recording | Electron dose: 131 e/Å2 / Film or detector model: GATAN ULTRASCAN 4000 (4k x 4k) |
Image scans | Num. digital images: 9 |
Radiation wavelength | Relative weight: 1 |
-Processing
EM software |
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3D reconstruction | Method: SUBTOMOGRAM AVERAGING / Num. of particles: 911 / Nominal pixel size: 6.5 Å Details: SUBTOMOGRAM AVERAGING. NOTE THE COORDINATES ARE BASED ON MODELLING. SUBMISSION BASED ON EXPERIMENTAL DATA FROM EMDB EMD-2369. (DEPOSITION ID: 11640). Symmetry type: HELICAL | ||||||||||||
Atomic model building | Protocol: OTHER / Details: METHOD--MODELLING REFINEMENT PROTOCOL--MODEL | ||||||||||||
Atomic model building | PDB-ID: 2WJ8 Accession code: 2WJ8 / Source name: PDB / Type: experimental model | ||||||||||||
Refinement step | Cycle: LAST
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