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- PDB-4bk7: Crystal Structure of a variant of the Major Birch Pollen Allergen... -
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Open data
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Basic information
Entry | Database: PDB / ID: 4bk7 | ||||||
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Title | Crystal Structure of a variant of the Major Birch Pollen Allergen Bet v 1 | ||||||
![]() | MAJOR POLLEN ALLERGEN BET V 1-A | ||||||
![]() | ALLERGEN / PR-10 | ||||||
Function / homology | ![]() response to biotic stimulus / abscisic acid binding / abscisic acid-activated signaling pathway / protein phosphatase inhibitor activity / defense response / signaling receptor activity / nucleus / cytoplasm Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Kofler, S. / Brandstetter, H. | ||||||
![]() | ![]() Title: Stabilization of the Dimeric Birch Pollen Allergen Bet V 1 Impacts its Immunological Properties Authors: Kofler, S.G. / Ackaert, C. / Samonig, M. / Asam, C. / Briza, P. / Horeis-Hoeck, J. / Cabrele, C. / Ferreira, F. / Duschl, A. / Huber, C. / Brandstetter, H. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 91.7 KB | Display | ![]() |
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PDB format | ![]() | 69.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 450.1 KB | Display | ![]() |
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Full document | ![]() | 451.1 KB | Display | |
Data in XML | ![]() | 10.5 KB | Display | |
Data in CIF | ![]() | 15 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 4bk6C ![]() 4bkcC ![]() 4bkdC ![]() 4a88S C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 17445.594 Da / Num. of mol.: 1 / Mutation: YES Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() | ||||||||
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#2: Chemical | ChemComp-SO4 / #3: Chemical | #4: Chemical | ChemComp-NA / | #5: Water | ChemComp-HOH / | Sequence details | THE UNIPROT SEQUENCE IS FURTHER PROCESSED INTO A MATURE FORM. STARTING METHIONINE OF MATURE PROTEIN ...THE UNIPROT SEQUENCE IS FURTHER PROCESSED INTO A MATURE FORM. STARTING METHIONINE | |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 1.99 Å3/Da / Density % sol: 38.08 % / Description: NONE |
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Crystal grow | pH: 4.5 / Details: 2M AMMONIUM SULFATE, 1.5 % MPD, pH 4.5 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Sep 10, 2011 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.8865 Å / Relative weight: 1 |
Reflection | Resolution: 1.14→55.81 Å / Num. obs: 47976 / % possible obs: 96.3 % / Observed criterion σ(I): 0 / Redundancy: 3.2 % / Rmerge(I) obs: 0.04 / Net I/σ(I): 17.1 |
Reflection shell | Resolution: 1.14→1.2 Å / Redundancy: 2.4 % / Rmerge(I) obs: 0.27 / Mean I/σ(I) obs: 3.5 / % possible all: 80.7 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 4A88 Resolution: 1.14→38.04 Å / Cor.coef. Fo:Fc: 0.977 / Cor.coef. Fo:Fc free: 0.964 / SU B: 1.05 / SU ML: 0.023 / Cross valid method: THROUGHOUT / ESU R: 0.036 / ESU R Free: 0.039 / Stereochemistry target values: MAXIMUM LIKELIHOOD Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS. U VALUES REFINED INDIVIDUALLY
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 11.367 Å2
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Refinement step | Cycle: LAST / Resolution: 1.14→38.04 Å
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Refine LS restraints |
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