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- PDB-4big: Crystal structure of the conserved staphylococcal antigen 1B, Csa1B -
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Open data
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Basic information
Entry | Database: PDB / ID: 4big | ||||||
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Title | Crystal structure of the conserved staphylococcal antigen 1B, Csa1B | ||||||
![]() | UNCHARACTERIZED LIPOPROTEIN SAOUHSC_00053 | ||||||
![]() | IMMUNE SYSTEM / DOMAIN OF UNKNOWN FUNCTION 576 (DUF576) / TANDEM LIPOPROTEIN / PROTECTIVE IMMUNITY | ||||||
Function / homology | Csa family / Csa superfamily / Csa1 family / Prokaryotic membrane lipoprotein lipid attachment site profile. / plasma membrane / Uncharacterized lipoprotein SAOUHSC_00053![]() | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Malito, E. / Bottomley, M.J. / Schluepen, C. / Liberatori, S. | ||||||
![]() | ![]() Title: Mining the Bacterial Unknown Proteome: Identification and Characterization of a Novel Family of Highly Conserved Protective Antigens in Staphylococcus Aureus Authors: Schluepen, C. / Malito, E. / Marongiu, A. / Schirle, M. / Mcwhinnie, E. / Lo Surdo, P. / Biancucci, M. / Falugi, F. / Nardi-Dei, V. / Marchi, S. / Fontana, M.R. / Lombardi, B. / De Falco, M. ...Authors: Schluepen, C. / Malito, E. / Marongiu, A. / Schirle, M. / Mcwhinnie, E. / Lo Surdo, P. / Biancucci, M. / Falugi, F. / Nardi-Dei, V. / Marchi, S. / Fontana, M.R. / Lombardi, B. / De Falco, M.G. / Rinaudo, C.D. / Spraggon, G. / Nissum, M. / Bagnoli, F. / Grandi, G. / Bottomley, M.J. / Liberatori, S. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 96.2 KB | Display | ![]() |
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PDB format | ![]() | 78.1 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 427.4 KB | Display | ![]() |
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Full document | ![]() | 430.2 KB | Display | |
Data in XML | ![]() | 9.8 KB | Display | |
Data in CIF | ![]() | 12.7 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Components
#1: Protein | Mass: 29462.051 Da / Num. of mol.: 1 / Fragment: RESIDUES 26-256 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
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#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.19 Å3/Da / Density % sol: 38.8 % / Description: NONE |
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Crystal grow | pH: 8.5 Details: 37% MPD_P1K_P3350, 0.1M MORPHEUS BUFFER 3, 10% MORPHEUS ALCOHOLS (CONDITION D12 OF MORPHEUS SCREEN FROM MOLECULAR DIMENSIONS), pH 8.5 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC CCD / Detector: CCD / Date: Jun 30, 2011 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9798 Å / Relative weight: 1 |
Reflection | Resolution: 2.27→50.34 Å / Num. obs: 11438 / % possible obs: 99.8 % / Observed criterion σ(I): 2 / Redundancy: 9.7 % / Biso Wilson estimate: 43.72 Å2 / Rmerge(I) obs: 0.09 / Net I/σ(I): 2 |
Reflection shell | Resolution: 2.27→2.4 Å / Redundancy: 9.7 % / Rmerge(I) obs: 0.36 / Mean I/σ(I) obs: 5.3 / % possible all: 99.6 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: NONE Resolution: 2.274→34.224 Å / SU ML: 0.29 / σ(F): 1.35 / Phase error: 29.19 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 52.64 Å2 | ||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.274→34.224 Å
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Refine LS restraints |
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LS refinement shell |
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Refinement TLS params. | Method: refined / Origin x: 12.4259 Å / Origin y: 25.2891 Å / Origin z: 5.3582 Å
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Refinement TLS group | Selection details: ALL |