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Yorodumi- PDB-4ay9: Structure of follicle-stimulating hormone in complex with the ent... -
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-Basic information
Entry | Database: PDB / ID: 4ay9 | ||||||
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Title | Structure of follicle-stimulating hormone in complex with the entire ectodomain of its receptor | ||||||
Components |
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Keywords | HORMONE/RECEPTOR / HORMONE-RECEPTOR COMPLEX / LEUCINE-RICH REPEATS / LRR / GPCR | ||||||
Function / homology | Function and homology information follicle-stimulating hormone receptor activity / progesterone biosynthetic process / follicle-stimulating hormone activity / follicle-stimulating hormone complex / pituitary gonadotropin complex / luteinizing hormone secretion / follicle-stimulating hormone secretion / positive regulation of steroid biosynthetic process / Thyroxine biosynthesis / Mineralocorticoid biosynthesis ...follicle-stimulating hormone receptor activity / progesterone biosynthetic process / follicle-stimulating hormone activity / follicle-stimulating hormone complex / pituitary gonadotropin complex / luteinizing hormone secretion / follicle-stimulating hormone secretion / positive regulation of steroid biosynthetic process / Thyroxine biosynthesis / Mineralocorticoid biosynthesis / Hormone ligand-binding receptors / Glycoprotein hormones / Reactions specific to the complex N-glycan synthesis pathway / Androgen biosynthesis / follicle-stimulating hormone signaling pathway / female gamete generation / Sertoli cell proliferation / gonad development / negative regulation of organ growth / cellular response to follicle-stimulating hormone stimulus / regulation of osteoclast differentiation / regulation of protein kinase A signaling / thyroid hormone generation / G protein-coupled peptide receptor activity / regulation of signaling receptor activity / organ growth / female gonad development / thyroid gland development / positive regulation of bone resorption / TFAP2 (AP-2) family regulates transcription of growth factors and their receptors / hormone-mediated signaling pathway / transforming growth factor beta receptor signaling pathway / female pregnancy / adenylate cyclase-activating G protein-coupled receptor signaling pathway / hormone activity / Golgi lumen / male gonad development / G alpha (s) signalling events / spermatogenesis / positive regulation of ERK1 and ERK2 cascade / positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction / receptor complex / positive regulation of cell migration / G protein-coupled receptor signaling pathway / positive regulation of cell population proliferation / positive regulation of gene expression / positive regulation of transcription by RNA polymerase II / extracellular space / extracellular region / membrane / plasma membrane / cytoplasm Similarity search - Function | ||||||
Biological species | HOMO SAPIENS (human) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.5 Å | ||||||
Authors | Jiang, X. / Liu, H. / Chen, X. / He, X. | ||||||
Citation | Journal: Proc.Natl.Acad.Sci.USA / Year: 2012 Title: Structure of Follicle-Stimulating Hormone in Complex with the Entire Ectodomain of its Receptor. Authors: Jiang, X. / Liu, H. / Chen, X. / Chen, P. / Fischer, D. / Sriraman, V. / Yu, H.N. / Arkinstall, S. / He, X. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 4ay9.cif.gz | 314.6 KB | Display | PDBx/mmCIF format |
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PDB format | pdb4ay9.ent.gz | 256.7 KB | Display | PDB format |
PDBx/mmJSON format | 4ay9.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ay/4ay9 ftp://data.pdbj.org/pub/pdb/validation_reports/ay/4ay9 | HTTPS FTP |
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-Related structure data
Related structure data | 1xwdS S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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2 |
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3 |
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Unit cell |
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-Components
#1: Protein | Mass: 10183.753 Da / Num. of mol.: 3 / Fragment: RESIDUES 25-116 Source method: isolated from a genetically manipulated source Source: (gene. exp.) HOMO SAPIENS (human) / Description: BACMAN SYSTEM / Plasmid: PVLAD6 / Cell line (production host): HEK293 / Production host: HOMO SAPIENS (human) / References: UniProt: Q96QJ4, UniProt: P01215*PLUS #2: Protein | Mass: 12500.208 Da / Num. of mol.: 3 Source method: isolated from a genetically manipulated source Source: (gene. exp.) HOMO SAPIENS (human) / Description: BACMAN SYSTEM / Plasmid: PVLAD6 / Cell line (production host): HEK293 / Production host: HOMO SAPIENS (human) / References: UniProt: P01225 #3: Protein | Mass: 40065.406 Da / Num. of mol.: 3 / Fragment: RESIDUES 17-366 Source method: isolated from a genetically manipulated source Source: (gene. exp.) HOMO SAPIENS (human) / Description: BACMAN SYSTEM / Plasmid: PVLAD6 / Cell line (production host): HEK293 / Production host: HOMO SAPIENS (human) / References: UniProt: P23945 #4: Sugar | ChemComp-NAG / #5: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.88 Å3/Da / Density % sol: 57.33 % / Description: NONE |
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Crystal grow | pH: 7.5 Details: 0.1M HEPES PH 7.5, 10% (V/V) ISOPROPANOL AND 20% (W/V) POLYETHYLENE GLYCOL 4000 |
-Data collection
Diffraction | Mean temperature: 110 K |
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Diffraction source | Source: SYNCHROTRON / Site: APS / Beamline: 21-ID-D / Wavelength: 0.979 |
Detector | Type: MARRESEARCH MX-300 / Detector: CCD |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.979 Å / Relative weight: 1 |
Reflection | Resolution: 2.5→50 Å / Num. obs: 70869 / % possible obs: 97.4 % / Observed criterion σ(I): 0 / Redundancy: 6.4 % / Rmerge(I) obs: 0.07 / Net I/σ(I): 15.8 |
Reflection shell | Resolution: 2.5→2.6 Å / Redundancy: 5.1 % / Rmerge(I) obs: 0.4 / Mean I/σ(I) obs: 3.3 / % possible all: 95.8 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PDB ENTRY 1XWD Resolution: 2.5→47.9 Å / Cor.coef. Fo:Fc: 0.925 / Cor.coef. Fo:Fc free: 0.9 / SU B: 31.462 / SU ML: 0.297 / Cross valid method: THROUGHOUT / ESU R: 0.53 / ESU R Free: 0.302 / Stereochemistry target values: MAXIMUM LIKELIHOOD Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS. U VALUES RESIDUAL ONLY
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.4 Å / Solvent model: BABINET MODEL WITH MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 61.853 Å2
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Refinement step | Cycle: LAST / Resolution: 2.5→47.9 Å
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Refine LS restraints |
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