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- PDB-4aso: TubR bound to 24 bp of tubC from Bacillus thuringiensis serovar i... -
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Basic information
Entry | Database: PDB / ID: 4aso | ||||||
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Title | TubR bound to 24 bp of tubC from Bacillus thuringiensis serovar israelensis pBtoxis | ||||||
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![]() | STRUCTURAL PROTEIN/DNA / STRUCTURAL PROTEIN-DNA COMPLEX / PARTITION / SEGREGATION | ||||||
Function / homology | plasmid partitioning / Winged helix DNA-binding domain superfamily / Winged helix-like DNA-binding domain superfamily / DNA binding / identical protein binding / DNA / DNA (> 10) / DNA-binding transcriptional repressor TubR![]() | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Aylett, C.H.S. / Lowe, J. | ||||||
![]() | ![]() Title: Superstructure of the Centromeric Complex of Tubzrc Plasmid Partitioning Systems. Authors: Aylett, C.H.S. / Lowe, J. | ||||||
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 384.7 KB | Display | ![]() |
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PDB format | ![]() | 307.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
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-Validation report
Summary document | ![]() | 514.5 KB | Display | ![]() |
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Full document | ![]() | 587 KB | Display | |
Data in XML | ![]() | 33.2 KB | Display | |
Data in CIF | ![]() | 53.1 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 4asnC ![]() 4assC ![]() 3m9aS C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
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Unit cell |
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Components
#1: Protein | Mass: 12119.912 Da / Num. of mol.: 16 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #2: DNA chain | Mass: 7323.761 Da / Num. of mol.: 4 / Fragment: SENSE STRAND / Source method: obtained synthetically / Source: (synth.) ![]() ![]() #3: DNA chain | Mass: 7408.855 Da / Num. of mol.: 4 / Fragment: ANTISENSE STRAND / Source method: obtained synthetically / Source: (synth.) ![]() ![]() Has protein modification | Y | Sequence details | BP 126549-126514 DNA IS CONTINUOUS IN THE CRYSTALS, BUT HAS BEEN CUT FOR REFINEMENT TO CENTRE THE ...BP 126549-126514 DNA IS CONTINUOUS | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 4.81 Å3/Da / Density % sol: 74.54 % Description: RIGID BODY ON VERY LOW RESOLUTION DATA. STRUCTURE CONFIRMED BY MSE SAD ANOMALOUS DIFFERENCE MAP. REGISTER OF DNA WAS INFERRED BY MICROARRAY EXPERIMENT. |
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Crystal grow | pH: 7.5 Details: BT TUBR-TUBC CRYSTALS WERE PRODUCED IN 500 NL TO 500 NL PROTEIN TO PRECIPITANT DROPS: 10 MG/ML BT TUBR, 500 UM TUBC-24, 100 MM NA-HEPES PH 7.5, 5 MM MGCL2, 25 % (W/V) POLYACRYLIC ACID 5100 SODIUM SALT. |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: May 29, 2011 |
Radiation | Monochromator: GRAPHITE CRYSTAL / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9794 Å / Relative weight: 1 |
Reflection | Resolution: 7→257.87 Å / Num. obs: 8779 / % possible obs: 98.1 % / Observed criterion σ(I): 1.3 / Redundancy: 5.9 % / Rmerge(I) obs: 0.09 / Net I/σ(I): 10.9 |
Reflection shell | Resolution: 7→7.38 Å / Redundancy: 5.9 % / Rmerge(I) obs: 1.642 / Mean I/σ(I) obs: 1.3 / % possible all: 98.5 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 3M9A Resolution: 7→64.469 Å / SU ML: 1.48 / σ(F): 1.34 / Phase error: 42.71 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 7→64.469 Å
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Refine LS restraints |
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LS refinement shell |
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