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- PDB-4ano: Crystal Structure Geobacillus thermodenitrificans EssB cytoplasmi... -
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Open data
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Basic information
Entry | Database: PDB / ID: 4ano | ||||||
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Title | Crystal Structure Geobacillus thermodenitrificans EssB cytoplasmic fragment | ||||||
![]() | ESSB | ||||||
![]() | MEMBRANE PROTEIN / MEMBRANE SECRETION / ESS TYPE V SECRETION SYSTEM | ||||||
Function / homology | ![]() | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Zoltner, M. / Fyfe, P. / Hunter, W.N. | ||||||
![]() | ![]() Title: The Architecture of Essb, an Integral Membrane Component of the Type Vii Secretion System. Authors: Zoltner, M. / Norman, D.G. / Fyfe, P.K. / El Mkami, H. / Palmer, T. / Hunter, W.N. | ||||||
History |
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Remark 650 | HELIX DETERMINATION METHOD: AUTHOR PROVIDED. | ||||||
Remark 700 | SHEET DETERMINATION METHOD: AUTHOR PROVIDED. |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 123.9 KB | Display | ![]() |
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PDB format | ![]() | 103.4 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 427.7 KB | Display | ![]() |
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Full document | ![]() | 434.2 KB | Display | |
Data in XML | ![]() | 14.1 KB | Display | |
Data in CIF | ![]() | 20.9 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Components
#1: Protein | Mass: 25661.123 Da / Num. of mol.: 1 / Fragment: CYTOPLASMIC FRAGMENT, RESIDUES 2-216 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Production host: ![]() ![]() |
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#2: Chemical | ChemComp-CL / |
#3: Chemical | ChemComp-NA / |
#4: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 1.59 Å3/Da / Density % sol: 14 % / Description: NONE |
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Crystal grow | pH: 5.5 Details: 0.1 M TRI-SODIUM CITRATE PH5.5, 20% PEG3000, 0.25MM TRIS(2-CARBOXYETHYL)PHOSPHINE HYDROCHLORIDE |
-Data collection
Diffraction | Mean temperature: 293 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 315r / Detector: CCD / Date: Feb 13, 2010 / Details: MIRRORS |
Radiation | Monochromator: SILICON / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9801 Å / Relative weight: 1 |
Reflection | Resolution: 1.7→44.61 Å / Num. obs: 26751 / % possible obs: 100 % / Observed criterion σ(I): 0 / Redundancy: 9.6 % / Rmerge(I) obs: 0.05 / Net I/σ(I): 24.4 |
Reflection shell | Resolution: 1.7→1.79 Å / Redundancy: 7.1 % / Rmerge(I) obs: 0.46 / Mean I/σ(I) obs: 4.3 / % possible all: 100 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: NONE Resolution: 1.7→44.61 Å / Cor.coef. Fo:Fc: 0.955 / Cor.coef. Fo:Fc free: 0.94 / SU B: 6.471 / SU ML: 0.098 / Cross valid method: THROUGHOUT / ESU R: 0.137 / ESU R Free: 0.134 / Stereochemistry target values: MAXIMUM LIKELIHOOD Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS. U VALUES WITH TLS ADDED
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.4 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 34.43 Å2
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Refinement step | Cycle: LAST / Resolution: 1.7→44.61 Å
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Refine LS restraints |
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