+Open data
-Basic information
Entry | Database: PDB / ID: 4ado | ||||||
---|---|---|---|---|---|---|---|
Title | Fusidic acid resistance protein FusB | ||||||
Components | FAR1 | ||||||
Keywords | ANTIBIOTIC RESISTANCE / PROTEIN BINDING PROTEIN | ||||||
Function / homology | Function and homology information | ||||||
Biological species | STAPHYLOCOCCUS AUREUS (bacteria) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.3 Å | ||||||
Authors | Guo, X. / Peisker, K. / Backbro, K. / Chen, Y. / Kiran, R.K. / Sanyal, S. / Selmer, M. | ||||||
Citation | Journal: Open Biol. / Year: 2012 Title: Structure and Function of Fusb: An Elongation Factor G-Binding Fusidic Acid Resistance Protein Active in Ribosomal Translocation and Recycling Authors: Guo, X. / Peisker, K. / Backbro, K. / Chen, Y. / Kiran, R.K. / Mandava, C.S. / Sanyal, S. / Selmer, M. | ||||||
History |
|
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
---|
-Downloads & links
-Download
PDBx/mmCIF format | 4ado.cif.gz | 180.9 KB | Display | PDBx/mmCIF format |
---|---|---|---|---|
PDB format | pdb4ado.ent.gz | 151.7 KB | Display | PDB format |
PDBx/mmJSON format | 4ado.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ad/4ado ftp://data.pdbj.org/pub/pdb/validation_reports/ad/4ado | HTTPS FTP |
---|
-Related structure data
-Links
-Assembly
Deposited unit |
| ||||||||
---|---|---|---|---|---|---|---|---|---|
1 |
| ||||||||
2 |
| ||||||||
Unit cell |
|
-Components
#1: Protein | Mass: 26313.334 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) STAPHYLOCOCCUS AUREUS (bacteria) / Production host: ESCHERICHIA COLI (E. coli) / Strain (production host): BL21 / References: UniProt: Q8GNY5 #2: Chemical | #3: Water | ChemComp-HOH / | |
---|
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
---|
-Sample preparation
Crystal | Density Matthews: 2.12 Å3/Da / Density % sol: 41.93 % / Description: NONE |
---|
-Data collection
Diffraction | Mean temperature: 100 K |
---|---|
Diffraction source | Source: SYNCHROTRON / Site: ESRF / Beamline: ID23-1 / Wavelength: 0.95372 |
Detector | Type: ADSC QUANTUM 315r / Detector: CCD |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.95372 Å / Relative weight: 1 |
Reflection | Resolution: 2.3→48 Å / Num. obs: 18111 / % possible obs: 94.5 % / Observed criterion σ(I): 3.67 / Redundancy: 3.86 % / Biso Wilson estimate: 33.48 Å2 / Rmerge(I) obs: 0.07 / Net I/σ(I): 14.38 |
Reflection shell | Resolution: 2.3→2.35 Å / Redundancy: 3.95 % / Mean I/σ(I) obs: 3.67 / % possible all: 95.9 |
-Processing
Software |
| |||||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method to determine structure: MOLECULAR REPLACEMENT / Resolution: 2.3→27.872 Å / SU ML: 0.34 / σ(F): 1.99 / Phase error: 26.76 / Stereochemistry target values: ML
| |||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | Shrinkage radii: 0.83 Å / VDW probe radii: 1.1 Å / Solvent model: FLAT BULK SOLVENT MODEL / Bsol: 43.944 Å2 / ksol: 0.343 e/Å3 | |||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters |
| |||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.3→27.872 Å
| |||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints |
| |||||||||||||||||||||||||||||||||||||||||||||||||
LS refinement shell |
|