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- PDB-4a2e: Crystal Structure of a Coriolopsis gallica Laccase at 1.7 A Resol... -
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Open data
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Basic information
Entry | Database: PDB / ID: 4a2e | ||||||||||||
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Title | Crystal Structure of a Coriolopsis gallica Laccase at 1.7 A Resolution pH 5.5 | ||||||||||||
![]() | LACCASE | ||||||||||||
![]() | OXIDOREDUCTASE / METAL-BINDING / BLUE MULTICOPPER OXIDASE | ||||||||||||
Function / homology | ![]() hydroquinone:oxygen oxidoreductase activity / laccase / copper ion binding / extracellular region Similarity search - Function | ||||||||||||
Biological species | ![]() | ||||||||||||
Method | ![]() ![]() | ||||||||||||
![]() | De La Mora, E. / Valderrama, B. / Horjales, E. / Rudino-Pinera, E. | ||||||||||||
![]() | ![]() Title: Structural Changes Caused by Radiation-Induced Reduction and Radiolysis: The Effect of X-Ray Absorbed Dose in a Fungal Multicopper Oxidase Authors: De La Mora, E. / Lovett, J.E. / Blanford, C.F. / Garman, E.F. / Valderrama, B. / Rudino-Pinera, E. | ||||||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 126.5 KB | Display | ![]() |
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PDB format | ![]() | 93.9 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 1.1 MB | Display | ![]() |
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Full document | ![]() | 1.1 MB | Display | |
Data in XML | ![]() | 26.6 KB | Display | |
Data in CIF | ![]() | 41.6 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 4a2dC ![]() 4a2fC ![]() 4a2gC ![]() 4a2hC ![]() 1v10S C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 52802.406 Da / Num. of mol.: 1 / Fragment: RESIDUES 22-517 / Source method: isolated from a natural source / Source: (natural) ![]() | ||||
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#2: Polysaccharide | Source method: isolated from a genetically manipulated source #3: Chemical | ChemComp-CU / #4: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.56 Å3/Da / Density % sol: 65.4 % / Description: NONE |
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Crystal grow | pH: 5.5 / Details: PEG 1000, PEG8000., pH 5.5 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() |
Detector | Type: RIGAKU-MSC / Detector: IMAGE PLATE / Date: Oct 17, 2006 / Details: YALE MIRRORS |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 1.8→25.63 Å / Num. obs: 63965 / % possible obs: 96.61 % / Observed criterion σ(I): 2.2 / Redundancy: 3.2 % / Biso Wilson estimate: 17.71 Å2 / Rmerge(I) obs: 0.05 / Net I/σ(I): 9.5 |
Reflection shell | Resolution: 1.8→1.847 Å / Redundancy: 2.4 % / Rmerge(I) obs: 0.3 / Mean I/σ(I) obs: 2.2 / % possible all: 90.34 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 1V10 Resolution: 1.8→25.63 Å / Cor.coef. Fo:Fc: 0.962 / Cor.coef. Fo:Fc free: 0.944 / SU B: 1.643 / SU ML: 0.052 / Cross valid method: THROUGHOUT / ESU R: 0.089 / ESU R Free: 0.092 / Stereochemistry target values: MAXIMUM LIKELIHOOD Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS. U VALUES REFINED INDIVIDUALLY.
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.4 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 15.711 Å2
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Refinement step | Cycle: LAST / Resolution: 1.8→25.63 Å
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Refine LS restraints |
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