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- PDB-485d: A SULFATE POCKET FORMED BY THREE GOU PAIRS IN THE STRUCTURE OF A ... -
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Open data
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Basic information
Entry | Database: PDB / ID: 485d | ||||||||||||||||||
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Title | A SULFATE POCKET FORMED BY THREE GOU PAIRS IN THE STRUCTURE OF A NONAMERIC RNA | ||||||||||||||||||
![]() | RNA/DNA(5'-R(*![]() DNA-RNA HYBRID / RNA / DNA/RNA HYBRID / SULFATE ION / DNA-RNA HYBRID complex | Function / homology | DNA/RNA hybrid | ![]() Method | ![]() ![]() ![]() ![]() Masquida, B. / Sauter, C. / Westhof, E. | ![]() ![]() Title: A sulfate pocket formed by three GoU pairs in the 0.97 A resolution X-ray structure of a nonameric RNA. Authors: Masquida, B. / Sauter, C. / Westhof, E. History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 32.2 KB | Display | ![]() |
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PDB format | ![]() | 22 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 396.7 KB | Display | ![]() |
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Full document | ![]() | 401.4 KB | Display | |
Data in XML | ![]() | 4.6 KB | Display | |
Data in CIF | ![]() | 5.9 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components on special symmetry positions |
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Components
#1: DNA/RNA hybrid | Mass: 2815.743 Da / Num. of mol.: 2 / Source method: obtained synthetically #2: Chemical | ChemComp-SO4 / | #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 1.84 Å3/Da / Density % sol: 33.15 % | ||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | Temperature: 310 K / Method: vapor diffusion, hanging drop / pH: 6 Details: 2.2-2.6 M AMMONIUM SULFATE, 5-50 MM MAGNESIUM SULFATE, 50 MM SODIUM CACODYLATE, 1MM SPERMINE, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 310K | ||||||||||||||||||||||||||||||||||||||||||
Components of the solutions |
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Crystal grow | *PLUS | ||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 110 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Detector: AREA DETECTOR |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9343 Å / Relative weight: 1 |
Reflection | Resolution: 0.97→20 Å / Num. all: 23838 / Num. obs: 23838 / % possible obs: 99.8 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 5.91 % / Rmerge(I) obs: 0.041 |
Reflection shell | Resolution: 0.97→0.99 Å / Redundancy: 2.63 % / Rmerge(I) obs: 0.446 / % possible all: 99.9 |
Reflection | *PLUS Num. measured all: 140902 |
Reflection shell | *PLUS % possible obs: 99.9 % |
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Processing
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Refinement | Method to determine structure: ![]()
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Refinement step | Cycle: LAST / Resolution: 0.97→20 Å
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Refine LS restraints |
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