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- PDB-3w24: The high-resolution crystal structure of TsXylA, intracellular xy... -
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Open data
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Basic information
Entry | Database: PDB / ID: 3w24 | ||||||
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Title | The high-resolution crystal structure of TsXylA, intracellular xylanase from Thermoanaerobacterium saccharolyticum JW/SL-YS485 | ||||||
![]() | Glycoside hydrolase family 10 | ||||||
![]() | HYDROLASE / glycoside hydrolase / xylanase / thermophilic | ||||||
Function / homology | ![]() endo-1,4-beta-xylanase activity / endo-1,4-beta-xylanase / polysaccharide catabolic process / carbohydrate binding Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Han, X. / Gao, J. / Shang, N. / Huang, C.-H. / Ko, T.-P. / Zhu, Z. / Wiegel, J. / Shao, W. / Guo, R.-T. | ||||||
![]() | ![]() Title: Structural and functional analyses of catalytic domain of GH10 xylanase from Thermoanaerobacterium saccharolyticum JW/SL-YS485 Authors: Han, X. / Gao, J. / Shang, N. / Huang, C.-H. / Ko, T.-P. / Chen, C.C. / Chan, H.C. / Cheng, Y.S. / Zhu, Z. / Wiegel, J. / Luo, W. / Guo, R.-T. / Ma, Y. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 92.4 KB | Display | ![]() |
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PDB format | ![]() | 68.2 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 427.2 KB | Display | ![]() |
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Full document | ![]() | 428.7 KB | Display | |
Data in XML | ![]() | 20 KB | Display | |
Data in CIF | ![]() | 32.7 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 3w25C ![]() 3w26C ![]() 3w27C ![]() 3w28C ![]() 3w29C ![]() 2q8xS ![]() 3w2b C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components on special symmetry positions |
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Components
#1: Protein | Mass: 38063.055 Da / Num. of mol.: 1 / Fragment: UNP residues 349-684 / Mutation: P326L Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Strain: JW/SL-YS485 / Gene: Tsac_1459 / Plasmid: pET46a / Production host: ![]() ![]() |
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#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.67 Å3/Da / Density % sol: 54 % |
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Crystal grow | Temperature: 295 K / Method: vapor diffusion, sitting drop / pH: 8 Details: 0.1M Tris, 25% PEG 6000, 0.8M LiCl, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 315 / Detector: CCD / Date: Jun 6, 2011 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Relative weight: 1 |
Reflection | Resolution: 1.35→25 Å / Num. obs: 90031 / % possible obs: 97.6 % / Redundancy: 3.6 % / Rmerge(I) obs: 0.062 / Net I/σ(I): 21.8 |
Reflection shell | Resolution: 1.35→1.4 Å / Redundancy: 3.6 % / Rmerge(I) obs: 0.481 / Mean I/σ(I) obs: 3.8 / % possible all: 100 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 2Q8X Resolution: 1.35→23.63 Å / Stereochemistry target values: Engh & Huber
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Solvent computation | Bsol: 53.6594 Å2 | ||||||||||||||||||||
Displacement parameters | Biso mean: 15.9421 Å2
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Refinement step | Cycle: LAST / Resolution: 1.35→23.63 Å
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Refine LS restraints |
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