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Open data
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Basic information
Entry | Database: PDB / ID: 3vqk | ||||||
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Title | Small heat shock protein hsp14.0 of wild type | ||||||
![]() | Small heat shock protein StHsp14.0 | ||||||
![]() | CHAPERONE / alpha-crystallin domain | ||||||
Function / homology | ![]() response to salt stress / response to hydrogen peroxide / : / unfolded protein binding / protein folding / protein complex oligomerization / response to heat Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Hanazono, Y. / Takeda, K. / Miki, K. | ||||||
![]() | ![]() Title: Structural studies on the oligomeric transition of a small heat shock protein, StHsp14.0 Authors: Hanazono, Y. / Takeda, K. / Yohda, M. / Miki, K. | ||||||
History |
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Remark 650 | HELIX DETERMINATION METHOD: AUTHOR DETERMINED | ||||||
Remark 700 | SHEET DETERMINATION METHOD: AUTHOR DETERMINED |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 139.3 KB | Display | ![]() |
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PDB format | ![]() | 110.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 469.4 KB | Display | ![]() |
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Full document | ![]() | 573.8 KB | Display | |
Data in XML | ![]() | 39.5 KB | Display | |
Data in CIF | ![]() | 51.9 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 3vqlC ![]() 3vqmC ![]() 3aabS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 14064.229 Da / Num. of mol.: 6 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.36 Å3/Da / Density % sol: 47.98 % |
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Crystal grow | Temperature: 277 K / Method: vapor diffusion, sitting drop / pH: 8 Details: 10% PEG2000MME, 4% glycerol, 4% sucrose, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: RAYONIX MX-225 / Detector: CCD / Date: Jul 16, 2010 / Details: mirrors |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 4.5→50 Å / Num. obs: 4122 / % possible obs: 87.2 % / Observed criterion σ(I): 0 / Redundancy: 3.9 % / Rsym value: 0.037 / Net I/σ(I): 42.6 |
Reflection shell | Resolution: 4.5→4.66 Å / Redundancy: 2 % / Mean I/σ(I) obs: 2.7 / Num. unique all: 350 / Rsym value: 0.45 / % possible all: 76.1 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 3AAB Resolution: 4.5→37.13 Å / Rfactor Rfree error: 0.027 / Data cutoff high absF: 4571134.27 / Data cutoff low absF: 0 / Isotropic thermal model: RESTRAINED / Cross valid method: THROUGHOUT / Stereochemistry target values: ML / Details: BULK SOLVENT MODEL USED
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Solvent computation | Solvent model: FLAT MODEL / Bsol: 189.635 Å2 / ksol: 0.25 e/Å3 | |||||||||||||||||||||||||||
Displacement parameters | Biso mean: 278.2 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 4.5→37.13 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 4.5→4.78 Å / Rfactor Rfree error: 0.102 / Total num. of bins used: 6
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Xplor file |
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