- PDB-3v61: Structure of S. cerevisiae PCNA conjugated to SUMO on lysine 164 -
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基本情報
登録情報
データベース: PDB / ID: 3v61
タイトル
Structure of S. cerevisiae PCNA conjugated to SUMO on lysine 164
要素
Proliferating cell nuclear antigen
Ubiquitin-like protein SMT3
キーワード
PROTEIN BINDING/DNA BINDING PROTEIN / UBIQUITIN-LIKE PROTEIN PCNA / POST-TRANSLATIONAL MODIFICATION / DNA REPLICATION / DNA DAMAGE RESPONSE / SRS2 / NEM MODIFICATION ON PCNA CYS22 AND CYS81 / NUCLEAR / PROTEIN BINDING-DNA BINDING PROTEIN complex
機能・相同性
機能・相同性情報
SUMO is conjugated to E1 (UBA2:SAE1) / SUMOylation of nuclear envelope proteins / SUMO is transferred from E1 to E2 (UBE2I, UBC9) / SUMO is proteolytically processed / SUMOylation of transcription factors / Mismatch repair (MMR) directed by MSH2:MSH6 (MutSalpha) / Postmitotic nuclear pore complex (NPC) reformation / SUMOylation of transcription cofactors / meiotic mismatch repair / Processive synthesis on the lagging strand ...SUMO is conjugated to E1 (UBA2:SAE1) / SUMOylation of nuclear envelope proteins / SUMO is transferred from E1 to E2 (UBE2I, UBC9) / SUMO is proteolytically processed / SUMOylation of transcription factors / Mismatch repair (MMR) directed by MSH2:MSH6 (MutSalpha) / Postmitotic nuclear pore complex (NPC) reformation / SUMOylation of transcription cofactors / meiotic mismatch repair / Processive synthesis on the lagging strand / septin ring / Removal of the Flap Intermediate / SUMOylation of DNA damage response and repair proteins / E3 ubiquitin ligases ubiquitinate target proteins / Polymerase switching / SUMOylation of DNA replication proteins / positive regulation of DNA metabolic process / maintenance of DNA trinucleotide repeats / Translesion Synthesis by POLH / Translesion synthesis by REV1 / Translesion synthesis by POLK / Translesion synthesis by POLI / establishment of mitotic sister chromatid cohesion / PCNA complex / SUMOylation of SUMOylation proteins / Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks / Termination of translesion DNA synthesis / lagging strand elongation / SUMOylation of RNA binding proteins / postreplication repair / SUMOylation of chromatin organization proteins / silent mating-type cassette heterochromatin formation / mitotic sister chromatid cohesion / error-free translesion synthesis / DNA polymerase processivity factor activity / leading strand elongation / ubiquitin-like protein ligase binding / protein sumoylation / Dual incision in TC-NER / subtelomeric heterochromatin formation / mismatch repair / translesion synthesis / positive regulation of DNA repair / condensed nuclear chromosome / positive regulation of DNA replication / replication fork / nucleotide-excision repair / protein tag activity / mitotic cell cycle / chromosome, telomeric region / DNA binding / identical protein binding / nucleus 類似検索 - 分子機能
PCNA IS NORMALLY A TRIMER BUT NEM MODIFICATION DISRUPTS THE TRIMER AND CAUSES PCNA TO RUN AS A MONOMER ON GEL FILTRATION THIS SUMO-PCNA MONOMER CRYSTALLIZES BY REFORMING THE PCNA:PCNA PROTOMER BUT WITH A RIGHT HANDED HELICAL SCREW COINCIDENT WITH THE I41 SCREW AXIS THE UNIT CELL CONTAINS ONE TURN OF THIS HELICAL SCREW
解像度: 2.8→50 Å / Cor.coef. Fo:Fc: 0.93 / Cor.coef. Fo:Fc free: 0.918 / Occupancy max: 1 / Occupancy min: 0.5 / SU B: 9.592 / SU ML: 0.187 / 交差検証法: THROUGHOUT / σ(F): 0 / ESU R: 0.997 / ESU R Free: 0.347 / 立体化学のターゲット値: MAXIMUM LIKELIHOOD 詳細: NEM molecule has occupancy of 1 as mass spec suggested that this ligand is fully modified in the studied samples.
Rfactor
反射数
%反射
Selection details
Rfree
0.2541
617
4.9 %
RANDOM
Rwork
0.2142
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-
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obs
0.2163
12550
98.94 %
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溶媒の処理
イオンプローブ半径: 0.8 Å / 減衰半径: 0.8 Å / VDWプローブ半径: 1 Å / 溶媒モデル: MASK