+Open data
-Basic information
Entry | Database: PDB / ID: 3utx | ||||||
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Title | Crystal structure of bacteriorhodopsin mutant T46A | ||||||
Components | Bacteriorhodopsin | ||||||
Keywords | PROTON TRANSPORT / MEMBRANE PROTEIN / PHOTORECEPTOR PROTEIN / RETINAL PROTEIN / ION TRANSPORT / SENSORY TRANSDUCTION | ||||||
Function / homology | Function and homology information light-driven active monoatomic ion transmembrane transporter activity / photoreceptor activity / phototransduction / proton transmembrane transport / monoatomic ion channel activity / plasma membrane Similarity search - Function | ||||||
Biological species | Halobacterium sp. (Halophile) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.47 Å | ||||||
Authors | Cao, Z. / Bowie, J.U. | ||||||
Citation | Journal: Proc.Natl.Acad.Sci.USA / Year: 2012 Title: Shifting hydrogen bonds may produce flexible transmembrane helices. Authors: Cao, Z. / Bowie, J.U. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 3utx.cif.gz | 93.4 KB | Display | PDBx/mmCIF format |
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PDB format | pdb3utx.ent.gz | 76.5 KB | Display | PDB format |
PDBx/mmJSON format | 3utx.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 3utx_validation.pdf.gz | 752.5 KB | Display | wwPDB validaton report |
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Full document | 3utx_full_validation.pdf.gz | 766.7 KB | Display | |
Data in XML | 3utx_validation.xml.gz | 19.9 KB | Display | |
Data in CIF | 3utx_validation.cif.gz | 26.2 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ut/3utx ftp://data.pdbj.org/pub/pdb/validation_reports/ut/3utx | HTTPS FTP |
-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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2 |
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3 |
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Unit cell |
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-Components
#1: Protein | Mass: 26899.473 Da / Num. of mol.: 2 / Mutation: T46A Source method: isolated from a genetically manipulated source Source: (gene. exp.) Halobacterium sp. (Halophile) / Strain: ATCC 700922 / JCM 11081 / NRC-1 / Gene: bop, VNG_1467G / Production host: Halobacterium salinarum (Halophile) / Strain (production host): L33 / References: UniProt: P02945 #2: Chemical | #3: Chemical | ChemComp-D12 / | #4: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.36 Å3/Da / Density % sol: 47.85 % |
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Crystal grow | Temperature: 310 K / Method: bicelles, vapor diffusion, hanging drop / pH: 4 Details: 0.65M sodium phosphate, 0.95% triethylene glycerol, 0.008M 1,6-hexanediol, 4.3% DMPC, 1.5% CHAPSO, BICELLES, VAPOR DIFFUSION, HANGING DROP, temperature 310K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: APS / Beamline: 24-ID-C / Wavelength: 0.9792 Å |
Detector | Type: ADSC QUANTUM 315 / Detector: CCD / Date: Feb 22, 2010 |
Radiation | Monochromator: Si(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9792 Å / Relative weight: 1 |
Reflection | Resolution: 2.47→90 Å / Num. all: 18034 / Num. obs: 18034 / % possible obs: 100 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 |
Reflection shell | Resolution: 2.47→2.56 Å / % possible all: 99.8 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT / Resolution: 2.47→51.5 Å / σ(F): 0 / Stereochemistry target values: MAXIMUM LIKELIHOOD
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Refinement step | Cycle: LAST / Resolution: 2.47→51.5 Å
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Refine LS restraints |
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