+
Open data
-
Basic information
Entry | Database: PDB / ID: 3utx | ||||||
---|---|---|---|---|---|---|---|
Title | Crystal structure of bacteriorhodopsin mutant T46A | ||||||
![]() | Bacteriorhodopsin | ||||||
![]() | PROTON TRANSPORT / MEMBRANE PROTEIN / PHOTORECEPTOR PROTEIN / RETINAL PROTEIN / ION TRANSPORT / SENSORY TRANSDUCTION | ||||||
Function / homology | ![]() photoreceptor activity / phototransduction / proton transmembrane transport / monoatomic ion channel activity / plasma membrane Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Cao, Z. / Bowie, J.U. | ||||||
![]() | ![]() Title: Shifting hydrogen bonds may produce flexible transmembrane helices. Authors: Cao, Z. / Bowie, J.U. | ||||||
History |
|
-
Structure visualization
Structure viewer | Molecule: ![]() ![]() |
---|
-
Downloads & links
-
Download
PDBx/mmCIF format | ![]() | 93.4 KB | Display | ![]() |
---|---|---|---|---|
PDB format | ![]() | 76.5 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 752.5 KB | Display | ![]() |
---|---|---|---|---|
Full document | ![]() | 766.7 KB | Display | |
Data in XML | ![]() | 19.9 KB | Display | |
Data in CIF | ![]() | 26.2 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
-
Links
-
Assembly
Deposited unit | ![]()
| ||||||||
---|---|---|---|---|---|---|---|---|---|
1 | ![]()
| ||||||||
2 | ![]()
| ||||||||
3 |
| ||||||||
Unit cell |
|
-
Components
#1: Protein | Mass: 26899.473 Da / Num. of mol.: 2 / Mutation: T46A Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() #2: Chemical | #3: Chemical | ChemComp-D12 / | #4: Water | ChemComp-HOH / | |
---|
-Experimental details
-Experiment
Experiment | Method: ![]() |
---|
-
Sample preparation
Crystal | Density Matthews: 2.36 Å3/Da / Density % sol: 47.85 % |
---|---|
Crystal grow | Temperature: 310 K / Method: bicelles, vapor diffusion, hanging drop / pH: 4 Details: 0.65M sodium phosphate, 0.95% triethylene glycerol, 0.008M 1,6-hexanediol, 4.3% DMPC, 1.5% CHAPSO, BICELLES, VAPOR DIFFUSION, HANGING DROP, temperature 310K |
-Data collection
Diffraction | Mean temperature: 100 K |
---|---|
Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 315 / Detector: CCD / Date: Feb 22, 2010 |
Radiation | Monochromator: Si(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9792 Å / Relative weight: 1 |
Reflection | Resolution: 2.47→90 Å / Num. all: 18034 / Num. obs: 18034 / % possible obs: 100 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 |
Reflection shell | Resolution: 2.47→2.56 Å / % possible all: 99.8 |
-
Processing
Software |
| ||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method to determine structure: ![]()
| ||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.47→51.5 Å
| ||||||||||||||||||||
Refine LS restraints |
|