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- PDB-3uso: Crystal structure of LeuT bound to L-selenomethionine in space gr... -
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Open data
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Basic information
Entry | Database: PDB / ID: 3uso | ||||||
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Title | Crystal structure of LeuT bound to L-selenomethionine in space group P21212 from lipid bicelles | ||||||
![]() | Transporter | ||||||
![]() | TRANSPORT PROTEIN / Leucine transporter | ||||||
Function / homology | Sodium:neurotransmitter symporter / Sodium:neurotransmitter symporter superfamily / Sodium:neurotransmitter symporter family / Sodium:neurotransmitter symporter family profile. / sodium ion transmembrane transport / plasma membrane / SELENOMETHIONINE / Na(+):neurotransmitter symporter (Snf family)![]() | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Wang, H. / Elferich, J. / Gouaux, E. | ||||||
![]() | ![]() Title: Structures of LeuT in bicelles define conformation and substrate binding in a membrane-like context. Authors: Wang, H. / Elferich, J. / Gouaux, E. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 201.2 KB | Display | ![]() |
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PDB format | ![]() | 162.1 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 3usgC ![]() 3usiC ![]() 3usjC ![]() 3uskC ![]() 3uslC ![]() 3usmC ![]() 3uspC ![]() 2a65S C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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3 |
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS domain:
NCS domain segments:
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Components
#1: Protein | Mass: 58077.438 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #2: Chemical | ChemComp-NA / #3: Chemical | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.76 Å3/Da / Density % sol: 67.27 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 5.1 Details: 100 mM NaAc, 32% MPD, 8.5% PEG400, 50 mM MgCl2, 100 mM Urea, pH 5.1, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 315r / Detector: CCD / Date: Jun 4, 2011 |
Radiation | Monochromator: Double crystal, Si(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97 Å / Relative weight: 1 |
Reflection | Resolution: 4.5→40 Å / Num. all: 94996 / Num. obs: 10449 / % possible obs: 94.6 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 9.1 % / Rmerge(I) obs: 0.09 / Net I/σ(I): 18.4 |
Reflection shell | Resolution: 4.5→4.66 Å / Redundancy: 7.3 % / Rmerge(I) obs: 0.26 / Mean I/σ(I) obs: 6.2 / % possible all: 83.9 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: pdb entry 2A65 Resolution: 4.5→34.529 Å / SU ML: 0.51 / σ(F): 0 / Phase error: 34.19 / Stereochemistry target values: ML Details: DUE TO LOW RESOLUTION THE REFINEMENT HAS BEEN PERFORMED HAS RIGID BODY ONLY
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Solvent computation | Shrinkage radii: 0.95 Å / VDW probe radii: 1.2 Å / Solvent model: FLAT BULK SOLVENT MODEL / Bsol: 80 Å2 / ksol: 0.272 e/Å3 | ||||||||||||||||||||||||
Displacement parameters |
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Refinement step | Cycle: LAST / Resolution: 4.5→34.529 Å
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Refine LS restraints |
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Refine LS restraints NCS |
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