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Open data
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Basic information
Entry | Database: PDB / ID: 3u3i | ||||||
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Title | A RNA binding protein from Crimean-Congo hemorrhagic fever virus | ||||||
![]() | Nucleocapsid protein | ||||||
![]() | RNA BINDING PROTEIN / nucleocapsid protein / endonuclease / RNA binding / CCHFV | ||||||
Function / homology | Methane Monooxygenase Hydroxylase; Chain G, domain 1 - #1110 / Nucleocapsid N protein / Nucleocapsid N protein / helical viral capsid / Methane Monooxygenase Hydroxylase; Chain G, domain 1 / viral nucleocapsid / Up-down Bundle / Mainly Alpha / Nucleocapsid protein![]() | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Guo, Y. / Wang, W.M. / Ji, W. / Deng, M. / Sun, Y.N. / Lou, Z.Y. / Rao, Z.H. | ||||||
![]() | ![]() Title: Crimean-Congo hemorrhagic fever virus nucleoprotein reveals endonuclease activity in bunyaviruses Authors: Guo, Y. / Wang, W.M. / Ji, W. / Deng, M. / Sun, Y.N. / Zhou, H. / Yang, C. / Deng, F. / Wang, H. / Hu, Z. / Lou, Z.Y. / Rao, Z.H. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 206.1 KB | Display | ![]() |
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PDB format | ![]() | 166.1 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 430.1 KB | Display | ![]() |
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Full document | ![]() | 470.9 KB | Display | |
Data in XML | ![]() | 27.8 KB | Display | |
Data in CIF | ![]() | 40.2 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 53984.297 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
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#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.41 Å3/Da / Density % sol: 49.02 % |
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Crystal grow | Temperature: 291 K / Method: vapor diffusion, hanging drop / pH: 6.8 Details: KCl, 20%(w/v) PEG3350, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 315 / Detector: CCD / Date: Jul 10, 2010 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9798 Å / Relative weight: 1 |
Reflection | Resolution: 2.3→50 Å / Num. obs: 22556 / % possible obs: 99.5 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Biso Wilson estimate: 37.46 Å2 |
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Processing
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Refinement | Method to determine structure: ![]()
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL / Bsol: 61.356 Å2 / ksol: 0.275 e/Å3 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 209.3 Å2 / Biso mean: 49.847 Å2 / Biso min: 11.6 Å2
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Refinement step | Cycle: LAST / Resolution: 2.304→43.639 Å
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Refine LS restraints |
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LS refinement shell | Refine-ID: X-RAY DIFFRACTION / Total num. of bins used: 8
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Refinement TLS params. | Method: refined / Refine-ID: X-RAY DIFFRACTION
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Refinement TLS group |
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