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- PDB-3tf3: Crystal structure of metal-free Human Arginase I -

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Basic information

Entry
Database: PDB / ID: 3tf3
TitleCrystal structure of metal-free Human Arginase I
ComponentsArginase-1
KeywordsHYDROLASE / ARGINASE FOLD
Function / homology
Function and homology information


positive regulation of neutrophil mediated killing of fungus / negative regulation of T-helper 2 cell cytokine production / Urea cycle / arginase / arginase activity / : / urea cycle / response to nematode / negative regulation of type II interferon-mediated signaling pathway / defense response to protozoan ...positive regulation of neutrophil mediated killing of fungus / negative regulation of T-helper 2 cell cytokine production / Urea cycle / arginase / arginase activity / : / urea cycle / response to nematode / negative regulation of type II interferon-mediated signaling pathway / defense response to protozoan / negative regulation of activated T cell proliferation / L-arginine catabolic process / negative regulation of T cell proliferation / specific granule lumen / azurophil granule lumen / manganese ion binding / adaptive immune response / innate immune response / Neutrophil degranulation / extracellular space / extracellular region / nucleus / cytosol / cytoplasm
Similarity search - Function
Arginase / Ureohydrolase domain / Ureohydrolase, manganese-binding site / Arginase family signature. / Ureohydrolase / Arginase family / Arginase family profile. / Arginase; Chain A / Ureohydrolase domain superfamily / 3-Layer(aba) Sandwich / Alpha Beta
Similarity search - Domain/homology
Biological speciesHomo sapiens (human)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.64 Å
AuthorsD'Antonio, E.L. / Christianson, D.W.
CitationJournal: Biochemistry / Year: 2011
Title: Crystal structures of complexes with cobalt-reconstituted human arginase I.
Authors: D'Antonio, E.L. / Christianson, D.W.
History
DepositionAug 15, 2011Deposition site: RCSB / Processing site: RCSB
Revision 1.0Aug 31, 2011Provider: repository / Type: Initial release
Revision 1.1Sep 14, 2011Group: Database references
Revision 1.2Sep 28, 2011Group: Database references
Revision 1.3Sep 13, 2023Group: Data collection / Database references / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Assembly

Deposited unit
A: Arginase-1
B: Arginase-1


Theoretical massNumber of molelcules
Total (without water)69,5602
Polymers69,5602
Non-polymers00
Water3,729207
1
A: Arginase-1

A: Arginase-1

A: Arginase-1


Theoretical massNumber of molelcules
Total (without water)104,3403
Polymers104,3403
Non-polymers00
Water543
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
crystal symmetry operation2_545-y,x-y-1,z1
crystal symmetry operation3_655-x+y+1,-x,z1
Buried area5580 Å2
ΔGint-17 kcal/mol
Surface area32880 Å2
MethodPISA
2
B: Arginase-1

B: Arginase-1

B: Arginase-1


Theoretical massNumber of molelcules
Total (without water)104,3403
Polymers104,3403
Non-polymers00
Water543
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
crystal symmetry operation2_655-y+1,x-y,z1
crystal symmetry operation3_665-x+y+1,-x+1,z1
Buried area5610 Å2
ΔGint-15 kcal/mol
Surface area32220 Å2
MethodPISA
Unit cell
Length a, b, c (Å)90.860, 90.860, 69.879
Angle α, β, γ (deg.)90.00, 90.00, 120.00
Int Tables number143
Space group name H-MP3

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Components

#1: Protein Arginase-1 / Liver-type arginase / Type I arginase


Mass: 34779.879 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: ARG1 / Plasmid: pBS(KS) / Production host: Escherichia coli (E. coli) / Strain (production host): BL21(DE3) / References: UniProt: P05089, arginase
#2: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 207 / Source method: isolated from a natural source / Formula: H2O

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.39 Å3/Da / Density % sol: 48.62 %
Crystal growTemperature: 298 K / Method: vapor diffusion, hanging drop / pH: 7
Details: 0.1 M HEPES, 30% (v/v) Jeffamine ED-2001, 0.014 M thymine, 0.0001 M manganese chloride. following xtal growth, metal-free HAI crystals are prepared by soaking a single crystal in 0.015 M ...Details: 0.1 M HEPES, 30% (v/v) Jeffamine ED-2001, 0.014 M thymine, 0.0001 M manganese chloride. following xtal growth, metal-free HAI crystals are prepared by soaking a single crystal in 0.015 M dipicolinic acid, 0.015 M EDTA, 30% (v/v) Jeffamine ED-2001 for 1 week, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K

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Data collection

DiffractionMean temperature: 100 K
Diffraction sourceSource: SYNCHROTRON / Site: NSLS / Beamline: X29A / Wavelength: 1.075 Å
DetectorType: ADSC QUANTUM 315 / Detector: CCD / Date: Sep 22, 2010
Details: Cryogenically cooled double crystal monochrometer with horizontal focusing sagittal bend second mono crystal with 4:1 magnification ratio and vertically focusing mirror
RadiationMonochromator: SAGITALLY FOCUSED Si(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 1.075 Å / Relative weight: 1
ReflectionResolution: 1.64→50 Å / Num. obs: 77810 / % possible obs: 99.3 % / Observed criterion σ(F): 0 / Observed criterion σ(I): -3 / Redundancy: 9.8 % / Rmerge(I) obs: 0.08 / Rsym value: 0.08 / Net I/σ(I): 35.185
Reflection shellResolution: 1.64→1.7 Å / Redundancy: 9.8 % / Rmerge(I) obs: 0.43 / Mean I/σ(I) obs: 5.459 / Rsym value: 0.43 / % possible all: 95.4

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Processing

Software
NameClassification
CBASSdata collection
CNSrefinement
HKL-2000data reduction
HKL-2000data scaling
CNSphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: PDB ENTRY 2AEB

2aeb


Resolution: 1.64→50 Å / Isotropic thermal model: isotropic / Cross valid method: THROUGHOUT / σ(F): 0 / Stereochemistry target values: Engh & Huber
RfactorNum. reflectionSelection details
Rfree0.3099 6447 random
Rwork0.2879 --
all0.2879 79124 -
obs0.2879 77810 -
Displacement parametersBiso mean: 35 Å2
Refine analyze
FreeObs
Luzzati coordinate error0.32 Å0.3 Å
Luzzati d res low-5 Å
Luzzati sigma a0.18 Å0.21 Å
Refinement stepCycle: LAST / Resolution: 1.64→50 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms4778 0 0 207 4985
Refine LS restraints
Refine-IDTypeDev ideal
X-RAY DIFFRACTIONc_angle_deg1.5
X-RAY DIFFRACTIONc_bond_d0.008
X-RAY DIFFRACTIONc_dihedral_angle_d22.8
X-RAY DIFFRACTIONc_improper_angle_d0.88
LS refinement shellResolution: 1.64→1.7 Å
RfactorNum. reflection% reflection
Rfree0 0 -
Rwork0.355 --
obs-7136 90.4 %

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