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Open data
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Basic information
Entry | Database: PDB / ID: 3tat | ||||||
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Title | TYROSINE AMINOTRANSFERASE FROM E. COLI | ||||||
![]() | TYROSINE AMINOTRANSFERASE | ||||||
![]() | AMINOTRANSFERASE / AROMATIC SUBSTRATES / PLP ENZYME | ||||||
Function / homology | ![]() beta-methylphenylalanine transaminase / L-tyrosine biosynthetic process from chorismate via 4-hydroxyphenylpyruvate / aromatic-amino-acid transaminase activity / aromatic-amino-acid transaminase / L-phenylalanine-2-oxoglutarate transaminase activity / aspartate biosynthetic process / L-leucine:2-oxoglutarate aminotransferase activity / L-phenylalanine biosynthetic process from chorismate via phenylpyruvate / L-tyrosine-2-oxoglutarate transaminase activity / L-leucine biosynthetic process ...beta-methylphenylalanine transaminase / L-tyrosine biosynthetic process from chorismate via 4-hydroxyphenylpyruvate / aromatic-amino-acid transaminase activity / aromatic-amino-acid transaminase / L-phenylalanine-2-oxoglutarate transaminase activity / aspartate biosynthetic process / L-leucine:2-oxoglutarate aminotransferase activity / L-phenylalanine biosynthetic process from chorismate via phenylpyruvate / L-tyrosine-2-oxoglutarate transaminase activity / L-leucine biosynthetic process / pyridoxal phosphate binding / protein homodimerization activity / identical protein binding / cytoplasm / cytosol Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Ko, T.P. / Yang, W.Z. / Wu, S.P. / Tsai, H. / Yuan, H.S. | ||||||
![]() | ![]() Title: Crystallization and preliminary crystallographic analysis of the Escherichia coli tyrosine aminotransferase. Authors: Ko, T.P. / Wu, S.P. / Yang, W.Z. / Tsai, H. / Yuan, H.S. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 436.2 KB | Display | ![]() |
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PDB format | ![]() | 361.2 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 448.9 KB | Display | ![]() |
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Full document | ![]() | 687.6 KB | Display | |
Data in XML | ![]() | 77.8 KB | Display | |
Data in CIF | ![]() | 108.4 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 7aatS S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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3 | ![]()
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS domain:
NCS oper:
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Components
#1: Protein | Mass: 43579.652 Da / Num. of mol.: 6 Source method: isolated from a genetically manipulated source Details: CONTAINS A PYRIDOXAL 5'-PHOSPHATE (PLP) COVALENTLY BOUND TO LYS 258 Source: (gene. exp.) ![]() ![]() ![]() ![]() References: UniProt: P04693, aromatic-amino-acid transaminase #2: Chemical | ChemComp-PLP / |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.7 Å3/Da / Density % sol: 48 % Description: THE CRYSTAL DIFFRACTED ANISOTROPICALLY TO 3.5 A ALONG C-AXIS BUT ONLY TO 5.5 A ALONG A-B PLANE | ||||||||||||||||||||||||||||||||||||||||
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Crystal grow | pH: 6.8 / Details: pH 6.8 | ||||||||||||||||||||||||||||||||||||||||
Crystal | *PLUS | ||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Method: vapor diffusion, hanging drop | ||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() |
Detector | Type: RIGAKU RAXIS II / Detector: IMAGE PLATE / Date: Jan 19, 1998 / Details: MIRRORS |
Radiation | Monochromator: NI FILTER / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 3.5→30 Å / Num. obs: 13117 / % possible obs: 88.5 % / Redundancy: 5 % / Biso Wilson estimate: 60 Å2 / Rmerge(I) obs: 0.093 / Rsym value: 0.093 / Net I/σ(I): 14 |
Reflection shell | Resolution: 3.5→3.8 Å / Redundancy: 1 % / Rmerge(I) obs: 0.118 / Mean I/σ(I) obs: 5 / Rsym value: 0.118 / % possible all: 1.5 |
Reflection | *PLUS Num. measured all: 65956 |
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Processing
Software |
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 7AAT Resolution: 3.5→30 Å / Rfactor Rfree error: 0.014 / Data cutoff high absF: 1000000 / Data cutoff low absF: 0.001 / Isotropic thermal model: RESTRAINED / Cross valid method: THROUGHOUT / σ(F): 2 / Details: STRICT NCS CONSTRAINT
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Displacement parameters | Biso mean: 56.2 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 3.5→30 Å
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Refine LS restraints |
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Refine LS restraints NCS | Refine-ID: X-RAY DIFFRACTION / Rms dev Biso : _ / Rms dev position: _ / Weight Biso : _ / Weight position: _
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LS refinement shell | Resolution: 3.5→3.62 Å / Rfactor Rfree error: 0 / Total num. of bins used: 10
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Xplor file |
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Software | *PLUS Name: ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS Rfactor Rfree: 0.26 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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LS refinement shell | *PLUS Rfactor Rfree: 0 |