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- PDB-3t7s: Crystal structure of complex of SAM and BVU_3255, a methyltransfe... -
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Open data
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Basic information
Entry | Database: PDB / ID: 3t7s | ||||||
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Title | Crystal structure of complex of SAM and BVU_3255, a methyltransferase from Bacteroides vulgatus ATCC 8482 | ||||||
![]() | Putative methyltransferase | ||||||
![]() | TRANSFERASE / Small molecule methyltransferase / BVU_3255 / ROSSMANN FOLD / METHYLTRASNFERASE / SAM / Methylation | ||||||
Function / homology | ![]() | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Kumar, V. / Sivaraman, J. | ||||||
![]() | ![]() Title: Structural characterization of BVU_3255, a methyltransferase from human intestine antibiotic resistant pathogen Bacteroides vulgatus Authors: Kumar, V. / Sivaraman, J. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 211 KB | Display | ![]() |
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PDB format | ![]() | 167.1 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 1.4 MB | Display | ![]() |
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Full document | ![]() | 1.4 MB | Display | |
Data in XML | ![]() | 43 KB | Display | |
Data in CIF | ![]() | 59.3 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 3t7rC ![]() 3t7tC ![]() 3e7pS C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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2 | ![]()
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3 | ![]()
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4 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 30664.715 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Details: METHYLTRANSFERASE / Source: (gene. exp.) ![]() ![]() ![]() #2: Chemical | ChemComp-SAM / #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.12 Å3/Da / Density % sol: 42.05 % |
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, hanging drop Details: 150mM NH4Cl, 17% w/v PEG 3350, 3% v/v 2-Propanol, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 315 / Detector: CCD / Date: Dec 11, 2010 |
Radiation | Monochromator: Graphite / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 2.2→50 Å / Num. all: 53257 / Num. obs: 53257 / % possible obs: 100 % / Observed criterion σ(F): 1 / Observed criterion σ(I): 1 / Rsym value: 0.049 / Net I/σ(I): 21.05 |
Reflection shell | Resolution: 2.2→2.28 Å / Mean I/σ(I) obs: 4.5 / Rsym value: 0.374 / % possible all: 100 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 3E7P Resolution: 2.2→30 Å / Occupancy max: 1 / Occupancy min: 1 / Isotropic thermal model: Isotropic / Cross valid method: THROUGHOUT / σ(F): 3 / Stereochemistry target values: Engh & Huber
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Solvent computation | Bsol: 39.7729 Å2 | ||||||||||||||||||||||||||||
Displacement parameters | Biso max: 77.2 Å2 / Biso mean: 41.552 Å2 / Biso min: 15.78 Å2
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Refinement step | Cycle: LAST / Resolution: 2.2→30 Å
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Refine LS restraints |
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Xplor file |
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