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Open data
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Basic information
Entry | Database: PDB / ID: 3soz | ||||||
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Title | Cytoplasmic Protein STM1381 from Salmonella typhimurium LT2 | ||||||
![]() | Cytoplasmic Protein STM1381 | ||||||
![]() | Structural Genomics / Unknown Function / PSI-Biology / Midwest Center for Structural Genomics / MCSG / Structure and Function of Pathogen Secreted Proteins / Program for the Characterization of Secreted Effector Proteins / PCSEP | ||||||
Function / homology | STM3548-like / Putative glutamine amidotransferase / Putative glutamine amidotransferase / Class I glutamine amidotransferase (GATase) domain / Class I glutamine amidotransferase-like / Rossmann fold / 3-Layer(aba) Sandwich / Alpha Beta / Cytoplasmic protein![]() | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Joachimiak, A. / Duke, N.E.C. / Jedrzejczak, R. / Li, H. / Adkins, J. / Brown, R. / Midwest Center for Structural Genomics (MCSG) / Program for the Characterization of Secreted Effector Proteins (PCSEP) | ||||||
![]() | ![]() Title: Cytoplasmic Protein STM1381 from Salmonella typhimurium LT2 Authors: Joachimiak, A. / Duke, N.E.C. / Jedrzejczak, R. / Li, H. / Adkins, J. / Brown, R. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 152.6 KB | Display | ![]() |
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PDB format | ![]() | 127.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 459.1 KB | Display | ![]() |
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Full document | ![]() | 479.1 KB | Display | |
Data in XML | ![]() | 29.9 KB | Display | |
Data in CIF | ![]() | 40.8 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data | |
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Other databases |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Details | trimer in asymmetric unit |
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Components
#1: Protein | Mass: 28055.889 Da / Num. of mol.: 3 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Strain: LT2 / Gene: ORF 245, orf245, STM1381 / Plasmid: pMCSG7 / Production host: ![]() ![]() #2: Chemical | #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.05 Å3/Da / Density % sol: 59.72 % |
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Crystal grow | Temperature: 289 K / Method: vapor diffusion, sitting drop / pH: 4.5 Details: 0.1 M Acetate pH 4.5 0.8 M NaH2PO4/1.2 M K2HPO4 (Wizard 2, screen 35), VAPOR DIFFUSION, SITTING DROP, temperature 289.0K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 315r / Detector: CCD / Date: Apr 3, 2011 Details: Si (111) double crystal monochromator sagitally focussing mirror |
Radiation | Monochromator: Si (111) double crystal monochromator / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9791827 Å / Relative weight: 1 |
Reflection | Resolution: 2.6→100 Å / Num. all: 33198 / Num. obs: 33198 / Observed criterion σ(I): 3 / Redundancy: 7.6 % |
Reflection shell | Resolution: 2.6→2.62 Å / Num. unique all: 1569 |
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Processing
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Refinement | Method to determine structure: ![]()
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.4 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 54.948 Å2
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Refinement step | Cycle: LAST / Resolution: 2.6→86.26 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.599→2.666 Å / Total num. of bins used: 20
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