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- PDB-3rag: Crystal Structure of Uncharacterized protein Aaci_0196 from Alicy... -
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Open data
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Basic information
Entry | Database: PDB / ID: 3rag | ||||||
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Title | Crystal Structure of Uncharacterized protein Aaci_0196 from Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446 | ||||||
![]() | uncharacterized protein | ||||||
![]() | Structural Genomics / Unknown function / PSI-Biology / Midwest Center for Structural Genomics / MCSG / TRUCTURAL GENOMICS / PSI-BIO / PROTEIN STRUCTURE INITIATIVE / CENTER FOR STRUCTURAL GENOMICS / LPHA-BETA-ALPHA fold / CYTOSOL | ||||||
Function / homology | von Willebrand factor, type A domain / von Willebrand factor type A domain / VWFA domain profile. / von Willebrand factor, type A / von Willebrand factor A-like domain superfamily / Rossmann fold / 3-Layer(aba) Sandwich / Alpha Beta / VWFA domain-containing protein![]() | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Wu, R. / Bedearn, J. / Joachimiak, A. / Midwest Center for Structural Genomics (MCSG) | ||||||
![]() | ![]() Title: Crystal Structure of Uncharacterized protein Aaci_0196 from Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446 Authors: Wu, R. / Bedearn, J. / Joachimiak, A. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 185.1 KB | Display | ![]() |
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PDB format | ![]() | 155.2 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 446.5 KB | Display | ![]() |
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Full document | ![]() | 455.5 KB | Display | |
Data in XML | ![]() | 22.6 KB | Display | |
Data in CIF | ![]() | 31.8 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data | |
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Other databases |
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Links
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Assembly
Deposited unit | ![]()
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2 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 26218.412 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Strain: DSM 446 / Gene: Aaci_0196 / Plasmid: PMCSG7 / Production host: ![]() ![]() #2: Chemical | #3: Chemical | #4: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.54 Å3/Da / Density % sol: 51.62 % |
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Crystal grow | Temperature: 298 K / pH: 8.5 Details: 1.5M Na formate, 0.1M tris.Hcl, pH 8.5 , 15% PEG3350, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: SBC-3 / Detector: CCD / Date: Oct 12, 2010 / Details: MIRRORS |
Radiation | Monochromator: DOUBLE CRYSTAL MONOCHROMATOR / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97943 Å / Relative weight: 1 |
Reflection | Resolution: 1.8→50 Å / Num. obs: 90228 / % possible obs: 98.5 % / Redundancy: 9.3 % / Biso Wilson estimate: 32.97 Å2 / Rsym value: 0.09 / Net I/σ(I): 33.6 |
Reflection shell | Resolution: 1.8→1.83 Å / Redundancy: 9.6 % / Mean I/σ(I) obs: 3.5 / Rsym value: 0.782 / % possible all: 100 |
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Processing
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Refinement | Method to determine structure: ![]()
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Solvent computation | Shrinkage radii: 0.95 Å / VDW probe radii: 1.2 Å / Solvent model: FLAT BULK SOLVENT MODEL / Bsol: 59.97 Å2 / ksol: 0.4 e/Å3 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters |
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Refinement step | Cycle: LAST / Resolution: 1.8→42.95 Å
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Refine LS restraints |
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LS refinement shell |
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Refinement TLS params. | Method: refined / Refine-ID: X-RAY DIFFRACTION
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Refinement TLS group |
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