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Yorodumi- PDB-3p64: Time-dependent and Protein-directed In Situ Growth of Gold Nanopa... -
+Open data
-Basic information
Entry | Database: PDB / ID: 3p64 | ||||||
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Title | Time-dependent and Protein-directed In Situ Growth of Gold Nanoparticles in a Single Crystal of Lysozyme | ||||||
Components | Lysozyme C | ||||||
Keywords | HYDROLASE / Bio-Nano Hybrids / Gold Nanoparticles / Porous Materials | ||||||
Function / homology | Function and homology information Antimicrobial peptides / Neutrophil degranulation / beta-N-acetylglucosaminidase activity / cell wall macromolecule catabolic process / lysozyme / lysozyme activity / killing of cells of another organism / defense response to Gram-negative bacterium / defense response to Gram-positive bacterium / defense response to bacterium ...Antimicrobial peptides / Neutrophil degranulation / beta-N-acetylglucosaminidase activity / cell wall macromolecule catabolic process / lysozyme / lysozyme activity / killing of cells of another organism / defense response to Gram-negative bacterium / defense response to Gram-positive bacterium / defense response to bacterium / endoplasmic reticulum / extracellular space / identical protein binding / cytoplasm Similarity search - Function | ||||||
Biological species | Gallus gallus (chicken) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.3 Å | ||||||
Authors | Wei, H. / Wang, Z. / Zhang, J. / House, S. / Gao, Y.-G. / Yang, L. / Robinson, H. / Tan, L.H. / Xing, H. / Hou, C. ...Wei, H. / Wang, Z. / Zhang, J. / House, S. / Gao, Y.-G. / Yang, L. / Robinson, H. / Tan, L.H. / Xing, H. / Hou, C. / Robertson, I.M. / Zuo, J.-M. / Lu, Y. | ||||||
Citation | Journal: Nat Nanotechnol / Year: 2011 Title: Time-dependent, protein-directed growth of gold nanoparticles within a single crystal of lysozyme. Authors: Wei, H. / Wang, Z. / Zhang, J. / House, S. / Gao, Y.G. / Yang, L. / Robinson, H. / Tan, L.H. / Xing, H. / Hou, C. / Robertson, I.M. / Zuo, J.M. / Lu, Y. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 3p64.cif.gz | 39 KB | Display | PDBx/mmCIF format |
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PDB format | pdb3p64.ent.gz | 29.3 KB | Display | PDB format |
PDBx/mmJSON format | 3p64.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/p6/3p64 ftp://data.pdbj.org/pub/pdb/validation_reports/p6/3p64 | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Components on special symmetry positions |
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-Components
#1: Protein | Mass: 14331.160 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Gallus gallus (chicken) / References: UniProt: P00698, lysozyme | ||||||
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#2: Chemical | #3: Chemical | ChemComp-AU / | #4: Chemical | ChemComp-CL / | #5: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 1.99 Å3/Da / Density % sol: 38.17 % |
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Crystal grow | Temperature: 298 K / Method: evaporation / pH: 4.5 Details: 6.5% NaCl (w/v) in 0.1 M sodium acetate, pH 4.5, EVAPORATION, temperature 298K |
-Data collection
Diffraction | Mean temperature: 123.2 K |
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Diffraction source | Source: SYNCHROTRON / Site: NSLS / Beamline: X12C / Wavelength: 1.0988 Å |
Detector | Type: MAR CCD 165 mm / Detector: CCD / Date: Nov 11, 2009 |
Radiation | Monochromator: Si 111 CHANNEL / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.0988 Å / Relative weight: 1 |
Reflection | Resolution: 1.3→50 Å / Num. obs: 28400 / % possible obs: 99.8 % / Observed criterion σ(F): 1 / Observed criterion σ(I): 1 / Redundancy: 27.4 % / Rmerge(I) obs: 0.079 / Rsym value: 0.079 / Net I/σ(I): 48.8 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT / Resolution: 1.3→10 Å / Num. parameters: 4843 / Num. restraintsaints: 4122 / Cross valid method: FREE R / σ(F): 0 / σ(I): 2 / Stereochemistry target values: Engh & Huber Details: ANISOTROPIC SCALING APPLIED BY THE METHOD OF PARKIN, MOEZZI & HOPE, J.APPL.CRYST.28(1995)53-56
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Refine analyze | Num. disordered residues: 0 / Occupancy sum hydrogen: 0 / Occupancy sum non hydrogen: 1206 | |||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.3→10 Å
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Refine LS restraints |
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