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Open data
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Basic information
Entry | Database: PDB / ID: 3nvj | ||||||
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Title | Crystal structure of the C143A/C166A mutant of Ero1p | ||||||
![]() | Endoplasmic oxidoreductin-1 | ||||||
![]() | OXIDOREDUCTASE / flavoenzyme / FAD / disulfide bonds / ER | ||||||
Function / homology | ![]() Oxidoreductases; Acting on a sulfur group of donors; With a disulfide as acceptor / thiol oxidase activity / protein folding in endoplasmic reticulum / protein-disulfide reductase activity / FAD binding / endoplasmic reticulum membrane / endoplasmic reticulum Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Fass, D. / Vonshak, O. | ||||||
![]() | ![]() Title: Steps in reductive activation of the disulfide-generating enzyme Ero1p Authors: Heldman, N. / Vonshak, O. / Sevier, C.S. / Vitu, E. / Mehlman, T. / Fass, D. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 87.7 KB | Display | ![]() |
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PDB format | ![]() | 64.5 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 898.2 KB | Display | ![]() |
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Full document | ![]() | 917.8 KB | Display | |
Data in XML | ![]() | 18 KB | Display | |
Data in CIF | ![]() | 23.3 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 3m31C ![]() 1rq1S S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 45294.898 Da / Num. of mol.: 1 / Fragment: residues in UNP 56-424 / Mutation: C143A,C166A Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Gene: ERO1, YML130C, YM4987.05C / Plasmid: pGEX-4T1 / Production host: ![]() ![]() References: UniProt: Q03103, Oxidoreductases; Acting on a sulfur group of donors; With a disulfide as acceptor |
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#2: Chemical | ChemComp-NEN / |
#3: Chemical | ChemComp-FAD / |
#4: Chemical | ChemComp-CD / |
#5: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 4.64 Å3/Da / Density % sol: 73.51 % |
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-Data collection
Diffraction source | Source: ![]() |
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Detector | Type: RIGAKU RAXIS IV++ / Detector: IMAGE PLATE / Date: May 16, 2010 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.514 Å / Relative weight: 1 |
Reflection | Resolution: 3.2→50 Å / Num. all: 13439 / Num. obs: 13131 / % possible obs: 97.7 % / Observed criterion σ(F): 2 / Observed criterion σ(I): 1 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 1RQ1 Resolution: 3.2→50 Å / σ(F): 2
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Refinement step | Cycle: LAST / Resolution: 3.2→50 Å
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