- PDB-3nj2: Crystal structure of cce_0566 from the cyanobacterium Cyanothece ... -
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基本情報
登録情報
データベース: PDB / ID: 3nj2
タイトル
Crystal structure of cce_0566 from the cyanobacterium Cyanothece 51142, a protein associated with nitrogen fixation from the DUF269 family
要素
DUF269-containing protein
キーワード
UNKNOWN FUNCTION / cyanobacteria / circadium rhythms / nitrogen fixation
機能・相同性
Protein of unknown function DUF269 / Uncharacterised protein family UPF0460 / Protein of unknown function, DUF269 / Putative cytoplasmic protein / Orthogonal Bundle / Mainly Alpha / DUF269-containing protein
温度: 293 K / 手法: 蒸気拡散法, ハンギングドロップ法 / pH: 7.1 詳細: 0.2 M Ammonium phosphate monobasic, 0.1 M Tris pH 8.5, 50% v/v (+/-)-2-Methyl-2,4-pentanediol, protein in 100 mM NaCl, 20 mM Tris, 1 mM DTT, pH 7.1. Protein 2 mg/mL, Mix 1.5 uL of protein and ...詳細: 0.2 M Ammonium phosphate monobasic, 0.1 M Tris pH 8.5, 50% v/v (+/-)-2-Methyl-2,4-pentanediol, protein in 100 mM NaCl, 20 mM Tris, 1 mM DTT, pH 7.1. Protein 2 mg/mL, Mix 1.5 uL of protein and precipatant, VAPOR DIFFUSION, HANGING DROP, temperature 293K
解像度: 1.59→33 Å / Cor.coef. Fo:Fc: 0.954 / Cor.coef. Fo:Fc free: 0.928 / SU B: 1.926 / SU ML: 0.07 / 交差検証法: THROUGHOUT / σ(F): 0 / ESU R Free: 0.113 / 立体化学のターゲット値: MAXIMUM LIKELIHOOD / 詳細: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
Rfactor
反射数
%反射
Selection details
Rfree
0.25452
2057
5.1 %
RANDOM
Rwork
0.21
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obs
0.2123
38536
91.69 %
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all
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38536
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溶媒の処理
イオンプローブ半径: 0.8 Å / 減衰半径: 0.8 Å / VDWプローブ半径: 1.4 Å / 溶媒モデル: MASK