A: Endoglucanase D E: Endoglucanase D B: Endoglucanase D F: Endoglucanase D C: Endoglucanase D G: Endoglucanase D D: Endoglucanase D H: Endoglucanase D ヘテロ分子
AUTHORS CRYSTALLIZED THE SEQUENCE CORRESPONDING TO UNP P28623 RESIDUES 32-515. HOWEVER, THIS ENTRY ...AUTHORS CRYSTALLIZED THE SEQUENCE CORRESPONDING TO UNP P28623 RESIDUES 32-515. HOWEVER, THIS ENTRY WAS SPILT INTO 2 MOLECULES, (CHAINS A,B,C,D) AND (CHAINS E,F,G,H), SINCE THE FLEXIBLE LINKER (RESIDUES 377-408), JOINING THE N-TERMINAL DOMAIN TO THE C-TERMINAL DOMAIN WAS NOT OBSERVED IN THE ELECTRON DENSITY MAP. THUS, IT IS IMPOSSIBLE TO KNOW FOR SURE WHICH N-TERMINAL DOMAIN (A,B,C,D) IS ASSOCIATED WITH WHICH C-TERMINAL DOMAIN (E,F,G,H) IN THIS CASE.
配列の詳細
RESIDUES 'TTTPTTPTTPTTPTTPTTPTTPTTPTTPTTPQ' CONSTITUTE THE FLEXIBLE LINKER THAT WAS NOT OBSERVED IN ...RESIDUES 'TTTPTTPTTPTTPTTPTTPTTPTTPTTPTTPQ' CONSTITUTE THE FLEXIBLE LINKER THAT WAS NOT OBSERVED IN THE ELECTRON DENSITY MAP
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実験情報
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実験
実験
手法: X線回折 / 使用した結晶の数: 1
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試料調製
結晶
マシュー密度: 2.54 Å3/Da / 溶媒含有率: 51.59 %
結晶化
温度: 277 K / 手法: 蒸気拡散法, ハンギングドロップ法 / pH: 4.5 詳細: 27% Polyethylene glycol 3350, 0.1 M BisTris pH 5.5. Cryoprotected with 29% Polyethylene glycol 3350, 5% ethylene glycol, 0.1 M BisTris pH 5.5, vapor diffusion, hanging drop, temperature 277K, ...詳細: 27% Polyethylene glycol 3350, 0.1 M BisTris pH 5.5. Cryoprotected with 29% Polyethylene glycol 3350, 5% ethylene glycol, 0.1 M BisTris pH 5.5, vapor diffusion, hanging drop, temperature 277K, VAPOR DIFFUSION, HANGING DROP