- PDB-3n0u: Crystal structure of Tm1821, the 8-oxoguanine DNA glycosylase of ... -
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Open data
ID or keywords:
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Basic information
Entry
Database: PDB / ID: 3n0u
Title
Crystal structure of Tm1821, the 8-oxoguanine DNA glycosylase of Thermotoga maritima
Components
Probable N-glycosylase/DNA lyase
Keywords
hydrolase / lyase / structural genomics / ISFI / DNA REPAIR / 8-OXOGUANINE / base excision repair / PSI-2 / Protein Structure Initiative / Integrated Center for Structure and Function Innovation
Function / homology
Function and homology information
hydrolase activity, hydrolyzing N-glycosyl compounds / Hydrolases; Glycosylases; Hydrolysing N-glycosyl compounds / class I DNA-(apurinic or apyrimidinic site) endonuclease activity / DNA-(apurinic or apyrimidinic site) lyase / base-excision repair Similarity search - Function
8-oxoguanine DNA glycosylase/AP lyase Ogg / Helix-hairpin-Helix base-excision DNA repair enzymes (C-terminal) / Endonuclease Iii, domain 2 / Hypothetical protein; domain 2 / Helix-hairpin-helix, base-excision DNA repair, C-terminal / HhH-GPD domain / endonuclease III / DNA glycosylase / Endonuclease III; domain 1 / Orthogonal Bundle / Mainly Alpha Similarity search - Domain/homology
Mass: 18.015 Da / Num. of mol.: 677 / Source method: isolated from a natural source / Formula: H2O
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 2.26 Å3/Da / Density % sol: 45.64 %
Crystal grow
Temperature: 298 K / Method: vapor diffusion, hanging drop / pH: 4 Details: Crystallization drop was 1:1 mixture of protein and 1M lithium chloride, 0.1M tri-sodium citrate, and 20% (w/v) PEG 6000. The crystallization reservoir was 1.5 M NaCl. The crystal was cryo- ...Details: Crystallization drop was 1:1 mixture of protein and 1M lithium chloride, 0.1M tri-sodium citrate, and 20% (w/v) PEG 6000. The crystallization reservoir was 1.5 M NaCl. The crystal was cryo-protected with LV Cryo Oil., pH 4, VAPOR DIFFUSION, HANGING DROP, temperature 298K
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